Complete chloroplast genome of a medicinal species Polygonatum kingianum in China (Asparagaceae, Asparagales)

Abstract

2020) Complete chloroplast genome of a medicinal species Polygonatum kingianum in China (Asparagaceae, Asparagales), Mitochondrial DNA Part B, 5:1, 959-960, ABSTRACTPolygonatum kingianum is a medicinal and food plant distributed in most of countries throughout the temperate Northern Hemisphere. Here we report on the complete chloroplast (cp) genome sequence of P. kingianum. The cp genome is 155,399 bp in size and includes two inverted repeat regions of 52,7411 bp, which is separated by a large single-copy region of 84,234 bp and a small single copy region of 18,424 bp. A total of 130 genes were predicted, including 38 tRNA, 8 rRNA, and 84 proteincoding genes. Phylogenetic analysis placed P. kingianum under the subfamily Nolinoideae of the family Asparagaceae. ARTICLE HISTORY

The Complete Chloroplast Genome Sequence of the Speirantha gardenii: Comparative and Adaptive Evolutionary Analysis

“…So, in the present study, we constructed a maximum-likelihood phylogenetic tree based on concatenated 75 protein-coding genes of 19 Nolinoideae species and revealed that all the species formed a monophyletic group and S. gardenii formed a cluster with R. carnea, R. chinensis and C. keiskei. The previous studies also support this phylogenetic tree where similar results were obtained that showed weakly supported bootstrap value in some nodes of the Nolinoideae phylogeny [9][10][11][12][13][14][15]48]. The phylogenetic result also showed that Speirantha, Rohdea, Reineckea and Convallaria genus are constantly clustered in the same clade with a high-resolution value, even though high sequence divergence is noted in few protein-coding genes of these chloroplast genomes.…”

“…Until recently, only a few complete chloroplast genome sequences for this Nolinoideae subfamily have been deposited in GenBank, with the very first being that of Polygonatum cyrtonema in 2015. Owing to the development of high-throughput sequencing technologies, additional Nolinoideae chloroplast genomes were sequenced [9][10][11][12][13][14][15], but the genus Speirantha has to date remained unexplored. Therefore, in the present study, we sequenced the S. gardenii chloroplast genome and compared it with its closed related other Nolinoideae members.…”

“…The reason for this is that the Nolinoideae subfamily consists of three major tribe clades, namely, Polygonateae, Aspidistreae and Ophiopogoneae, and is weakly supported in the phylogenetic tree [47]. Moreover, in the Polygonateae clade, several Polygonatum species were characterized and their phylogenetic relationships have been resolved recently [9,11,14,15]. In contrast, only a few studies have been carried out in the Aspidistreae tribe clade [10,12,13].…”

“…Among these, Nolinoideae is a subfamily, with more than 100 species, of the 2 of 16 Asparagaceae family belonging to the monocot flowering plants. In the past decade, a few species of Nolinoideae have been characterized at the molecular level and phylogenetic implications with other species were identified [9][10][11][12][13][14][15]. The genus Speirantha belongs to the subfamily Nolinoideae and consists of only one species, S. gardenii and its common name is "False Lily of the Valley".…”

The complete chloroplast genome of Polygonatum cirrhifolium (Wall.) Royle, a medicine herb

“…Initial gene annotation was done by Geneious R11 v11.0.5 (Biomatters Ltd, Auckland, New Zealand) based on the chloroplast genome of P. kingianum (MN934979; Jin et al. 2020 ). Genes that could not detected were further identified by Blastn ( https://blast.ncbi.nlm.nih.gov/Blast.cgi ).…”