Complete Coding Sequence of Western Equine Encephalitis Virus Strain Fleming, Isolated from a Human Case

Burke, Crystal W.; Wiley, Michael R.; Beitzel, Brett; Gardner, Christina L.; Huang, Yan; Piper, Ashley E.; Vanlandingham, Dana L.; Higgs, Stephen; Palacios, Gustavo; Glass, Pamela J.

doi:10.1128/mra.01223-19

Cited by 6 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, a deletion in the nsP 4 region of WEEV Fleming was re-created using consensus amino acid sequence derived using the McMillan codon preference. However, subsequent to creation of the Fleming cDNA clone, a revised sequence of Fleming was published [ 42 ]. The sequence in the area of the deletion was identical to our inserted sequence.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Phenotypes of Venezuelan, Eastern and Western Equine Encephalitis Viruses Derived from cDNA Clones of Human Isolates

Gardner

Sun

Dunn

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

The Department of Defense recently began an effort to improve and standardize virus challenge materials and efficacy determination strategies for testing therapeutics and vaccines. This includes stabilization of virus genome sequences in cDNA form where appropriate, use of human-derived virus isolates, and noninvasive strategies for determination of challenge virus replication. Eventually, it is desired that these approaches will satisfy the FDA “Animal Rule” for licensure, which substitutes animal efficacy data when human data are unlikely to be available. To this end, we created and examined the virulence phenotype of cDNA clones of prototypic human infection-derived strains of the alphaviruses, Venezuelan (VEEV INH9813), eastern (EEEV V105) and western (WEEV Fleming) equine encephalitis viruses, and created fluorescent and luminescent reporter expression vectors for evaluation of replication characteristics in vitro and in vivo. Sequences of minimally passaged isolates of each virus were used to synthesize full-length cDNA clones along with a T7 transcription promoter-based bacterial propagation vector. Viruses generated from the cDNA clones were compared with other “wild type” strains derived from cDNA clones and GenBank sequences to identify and eliminate putative tissue culture artifacts accumulated in the cell passaged biological stocks. This was followed by examination of aerosol and subcutaneous infection and disease in mouse models. A mutation that increased heparan sulfate binding was identified in the VEEV INH9813 biological isolate sequence and eliminated from the cDNA clone. Viruses derived from the new human isolate cDNA clones showed similar mouse virulence to existing clone-derived viruses after aerosol or subcutaneous inoculation.

show abstract

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Phenotypes of Venezuelan, Eastern and Western Equine Encephalitis Viruses Derived from cDNA Clones of Human Isolates

Gardner

Sun

Dunn

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…Western equine encephalitis virus (WEEV) is a very important member of the alphavirus family. [1][2][3] WEEV causes severe encephalitis in humans and equines, and the case mortality rate is 1%-5%. [4] High mortality was also reported to be caused by aerosol transmission of WEEV in laboratory accidents.…”

Section: Introductionmentioning

confidence: 99%

Western equine encephalitis virus virus‐like particles from an insect cell‐baculovirus system elicit the strong immune responses in mice

Wang

Zheng

et al. 2021

Biotechnology Journal

View full text Add to dashboard Cite

Western equine encephalitis virus (WEEV) causes lethal encephalitis in humans and equines, and it poses a serious public health threat in many countries. Therefore, the development of an efficient vaccine remains an important challenge for the prevention of WEEV infection. This study presents the first description of WEEV virus‐like particles (VLPs) generated from insect cells using recombinant baculoviruses. WEEV VLPs with 206 adjuvant could trigger a strong cellular immune response; increase the levels of IL‐2, IL‐4 and IFN‐γ; and induce a high level of neutralizing antibodies against WEEV in mice. These data showed that the insect cell‐baculovirus system is suitable for the production of WEEV VLPs and that these VLPs could elicit the strong immunogenicity in mice. These results suggest a new, nonreplicating, and effective vaccine candidate against WEEV infection.

show abstract

Animal models of alphavirus infection and human disease

Lucas

Morrison

2022

Advances in Virus Research

View full text Add to dashboard Cite

Complete Coding Sequence of Western Equine Encephalitis Virus Strain Fleming, Isolated from a Human Case

Abstract: We sequenced the complete coding genome of the western equine encephalitis virus (WEEV) strain Fleming. This strain was originally isolated in 1938 from a human WEEV case.

Cited by 6 publications

References 14 publications

In Vitro and In Vivo Phenotypes of Venezuelan, Eastern and Western Equine Encephalitis Viruses Derived from cDNA Clones of Human Isolates

In Vitro and In Vivo Phenotypes of Venezuelan, Eastern and Western Equine Encephalitis Viruses Derived from cDNA Clones of Human Isolates

Western equine encephalitis virus virus‐like particles from an insect cell‐baculovirus system elicit the strong immune responses in mice

Animal models of alphavirus infection and human disease

Contact Info

Product

Resources

About