Complete degradation of dimethyl phthalate by biochemical cooperation of the Bacillus thuringiensis strain isolated from cotton field soil

Surhio, Muhammad Ali; Talpur, Farah Naz; Nizamani, Sarwat; Amin, Farah; Bong, Chui Wei; Lee, Choon Weng; Ashraf, Muhammad Aqeel; Shah, Muhammad Raza

doi:10.1039/c4ra09465d

Cited by 63 publications

(39 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, the bacterial consortium is the primary unit that per-forms biological functions, and most bacteria that survive in bacterial consortia depend on communication or substance exchange with other bacteria. Therefore, many degradation-associated microorganisms are not always found in monoculture (2-4) but rather occur in close association with other bacteria, forming microbial consortia that exhibit better biodegradation performance than single species (5,6). As reported previously, a microbial consortium composed of Gordonia sp.…”

mentioning

confidence: 87%

Metabolite Cross-Feeding between Rhodococcus ruber YYL and Bacillus cereus MLY1 in the Biodegradation of Tetrahydrofuran under pH Stress

Liu

Huang

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

Bacterial consortia are among the most basic units in the biodegradation of environmental pollutants. Pollutant-degrading strains frequently encounter different types of environmental stresses and must be able to survive with other bacteria present in the polluted environments. In this study, we proposed a noncontact interaction mode between a tetrahydrofuran (THF)-degrading strain, Rhodococcus ruber YYL, and a non-THF-degrading strain, Bacillus cereus MLY1. The metabolic interaction mechanism between strains YYL and MLY1 was explored through physiological and molecular studies and was further supported by the metabolic response profile of strain YYL, both monocultured and cocultured with strain MLY1 at the optimal pH (pH 8.3) and under pH stress (pH 7.0), through a liquid chromatography-mass spectrometry-based metabolomic analysis. The results suggested that the coculture system resists pH stress in three ways: (i) strain MLY1 utilized acid metabolites and impacted the proportion of glutamine, resulting in an elevated intracellular pH of the system; (ii) strain MLY1 had the ability to degrade intermediates, thus alleviating the product inhibition of strain YYL; and (iii) strain MLY1 produced some essential micronutrients for strain YYL to aid the growth of this strain under pH stress, while strain YYL produced THF degradation intermediates for strain MLY1 as major nutrients. In addition, a metabolite cross-feeding interaction with respect to pollutant biodegradation is discussed. IMPORTANCE Rhodococcus species have been discovered in diverse environmental niches and can degrade numerous recalcitrant toxic pollutants. However, the pollutant degradation efficiency of these strains is severely reduced due to the complexity of environmental conditions and limitations in the growth of the pollutant-degrading microorganism. In our study, Bacillus cereus strain MLY1 exhibited strong stress resistance to adapt to various environments and improved the THF degradation efficiency of Rhodococcus ruber YYL by a metabolic cross-feeding interaction style to relieve the pH stress. These findings suggest that metabolite cross-feeding occurred in a complementary manner, allowing a pollutant-degrading strain to collaborate with a nondegrading strain in the biodegradation of various recalcitrant compounds. The study of metabolic exchanges is crucial to elucidate mechanisms by which degrading and symbiotic bacteria interact to survive environmental stress.

show abstract

mentioning

confidence: 87%

Metabolite Cross-Feeding between Rhodococcus ruber YYL and Bacillus cereus MLY1 in the Biodegradation of Tetrahydrofuran under pH Stress

Liu

Huang

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The monomethyl phthalate (MMP) and PA found in the BER, using liquid chromatography-MS analysis (as shown in Fig. 8), proved that the conventional biodegradation path of DMP is DMP-MMP-PA. 33 The degradation of MMP and PA were rate-limiting steps in DMP degradation because they were difficult to turn into H 2 O and CO 2 . 34 Thus, a large amount of MMP and PA produced greater COD in Ana, which explained why the removal efficiency of COD in the Ana was lower than in the BER (Fig.…”

Section: The Probable Degradation Pathway Of Dmp In the Bermentioning

confidence: 99%

The efficient biodegradation of dimethyl phthalate using an anaerobic bioelectrochemical system

Zhou

Shi

Fan

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND Dimethyl phthalate (DMP) is refractory, persistent and easy to enrich in anaerobic environments. The urgent need to degrade this refractory organic matter with high efficiency can be met by bioelectrochemical technology. In this paper, the degradation characteristics of DMP under different reaction conditions in bioelectrical reactors (BERs) were studied. RESULTS With operation conditions of 400 mg L−1 DMP, 1.3 V applied voltage and 50 h HRT, the highest removal efficiency of DMP was obtained in a BER (84.0%), which was higher than a conventional anaerobic system (Ana) (68.0%). Phthalic acid, heptanedioic acid and other low molecular weight products of DMP were detected in the BER, which shows that the applied voltage can stimulate the intermediate products of DMP into further degradation. Simultaneously, the abundance of microorganisms in the BER was richer than in the Ana. CONCLUSION The higher removal efficiency of DMP, the further degradation of DMP and the richer abundance of bacteria in the BER indicate that electrochemical methods can promote the intensive removal of DMP. © 2019 Society of Chemical Industry

show abstract

“…25 Heptadecane was used as a model hydrocarbon to monitor biodegradation behavior accurately. Aeration rate, pulp density and inoculum content and their interactions were selected to determine process efficiency and optimal conditions to evaluate the ability of the isolated bacterium to degrade aliphatic hydrocarbons.…”

Section: Experimental Designmentioning

confidence: 99%

Biodegradation of heptadecane in hydrocarbon polluted dune sands using a newly-isolated thermophilic bacterium, Brevibacillus borstelensis TMU30: statistical evaluation and process optimization

et al. 2015

View full text Add to dashboard Cite

An enrichment culture was established to isolate a thermophilic hydrocarbon-degrading bacterium from contaminated soil samples from the Tehran Petroleum Refinery. The bacterium was characterized based on 16S rRNA and identified as Brevibacillus borstelensis TMU30. It is registered at NCBI under accession number KF181624.1. The capability of the bacterium for degradation of heptadecane as a representative contaminant in polluted dune sand was evaluated in a slurry bubble column bioreactor. The aeration rate, inoculum content and pulp density were optimized to maximize the degradation of heptadecane using the central composite design of response surface methodology. The results showed that maximum heptadecane reduction reached 48% at an aeration rate of 62 ml min À1 , inoculum content of 9.3% (v/v) and pulp density of 63 g l À1 only after 4 days. This study highlights an important potential use of thermophilic degradative bacteria to eliminate contamination in a slurry bioreactor while shortening dramatically the treatment time.

show abstract

Complete degradation of dimethyl phthalate by biochemical cooperation of the Bacillus thuringiensis strain isolated from cotton field soil

Cited by 63 publications

References 40 publications

Metabolite Cross-Feeding between Rhodococcus ruber YYL and Bacillus cereus MLY1 in the Biodegradation of Tetrahydrofuran under pH Stress

Metabolite Cross-Feeding between Rhodococcus ruber YYL and Bacillus cereus MLY1 in the Biodegradation of Tetrahydrofuran under pH Stress

The efficient biodegradation of dimethyl phthalate using an anaerobic bioelectrochemical system

Biodegradation of heptadecane in hydrocarbon polluted dune sands using a newly-isolated thermophilic bacterium, Brevibacillus borstelensis TMU30: statistical evaluation and process optimization

Contact Info

Product

Resources

About