Complete genome analysis of bacteriophage EFC1 infecting Enterococcus faecalis from chicken

Wang, Qi; Liu, Na

doi:10.1007/s00203-022-02838-5

Cited by 1 publication

(1 citation statement)

References 30 publications

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“…Interestingly, in contrast to the abolished Siphoviridae family, described as having genomes composed of linear, double stranded DNA (dsDNA), the genomes extracted from virions of the induced sakei strains were mostly marked as circular. A similar phage (with the siphovirus morphotype and a circular dsDNA genome) was found in the Enterococcus faecalis phage EFC1 which belongs to the new genus Saphexavirus [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Distribution, inducibility, and characterisation of prophages in Latilactobacillus sakei

Ambros

Ehrmann

2022

BMC Microbiol

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Background Lactic acid bacteria (LAB) are used as starters in a wide variety of food fermentations. While the number of reports of phages infecting other LAB steadily increased over the years, information about phage associated with Latilactobacillus sakei, a frequently used meat starter, remains scarce. Results In this study, a predictive genomic analysis of 43 Latilactobacillus sakei genomes revealed the presence of 26 intact, eleven questionable and 52 incomplete prophage sequences across all analysed genomes with a range of one to five predicted prophage sequences per strain. Screening 24 sakei strains for inducible prophages by utilising UV light or mitomycin C, we identified seven lysogenic strains showing lysis after induction during subsequent growth monitoring. Electron microscopic analysis revealed fully assembled virions in the purified lysates of four samples, thus confirming successful prophage induction. All virions featured icosahedral, isomeric heads and long, most likely non-contractile tails indicating siphoviruses. By performing phylogenetic analyses with various marker genes as well as full prophage sequences, we displayed a remarkably high diversity of prophages, that share a similar gene module organisation and six different chromosomal integration sites were identified. By sequencing viral DNA purified from lysates of Latilactobacillus sakei TMW 1.46, we demonstrate that simultaneous induction of multiple prophages is possible. Conclusions With this work, we not only provide data about the incidence of prophages harboured by the meat starter Latilactobacillus sakei, we also demonstrated their potential to impact growth of their host after induction, as well as forming seemingly fully assembled virions.

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Section: Discussionmentioning

confidence: 99%