Complete Genome Analysis of Rhodococcus opacus S8 Capable of Degrading Alkanes and Producing Biosurfactant Reveals Its Genetic Adaptation for Crude Oil Decomposition

Delegan, Yanina; Petrikov, Kirill; Frantsuzova, Ekaterina; Rudenko, Natalia N.; Solomentsev, Viktor I.; Сузина, Н. Е.; Travkin, V. M.; Solyanikova, Inna P.

doi:10.3390/microorganisms10061172

Cited by 8 publications

(7 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transmission electron microscopy observation of ultrathin sections of X5 cells grown on solid hexadecane showed that vesicles were connected to cells by means of electron-dense filaments-fibers (F) (Figure 5). Delegan et al [43] studied the ultrastructure of rhodococci cells grown on dodecane at 28 • C. They described surface fibrillar structures, which are most commonly used by bacterial cells to attach to the surface. In contrast, we showed that fibers were pili-like structures that were hollow and possibly served to transport hexadecane and its intermediates.…”

Section: Discussionmentioning

confidence: 99%

Hydrocarbons Biodegradation by Rhodococcus: Assimilation of Hexadecane in Different Aggregate States

et al. 2022

View full text Add to dashboard Cite

The aim of our study was to reveal the peculiarities of the adaptation of rhodococci to hydrophobic hydrocarbon degradation at low temperatures when the substrate was in solid states. The ability of actinobacteria Rhodococcus erythropolis (strains X5 and S67) to degrade hexadecane at 10 °C (solid hydrophobic substrate) and 26 °C (liquid hydrophobic substrate) is described. Despite the solid state of the hydrophobic substrate at 10 °C, bacteria demonstrate a high level of its degradation (30–40%) within 18 days. For the first time, we show that specialized cellular structures are formed during the degradation of solid hexadecane by Rhodococcus at low temperatures: intracellular multimembrane structures and surface vesicles connected to the cell by fibers. The formation of specialized cellular structures when Rhodococcus bacteria are grown on solid hexadecane is an important adaptive trait, thereby contributing to the enlargement of a contact area between membrane-bound enzymes and a hydrophobic substrate.

show abstract

Section: Discussionmentioning

confidence: 99%

Hydrocarbons Biodegradation by Rhodococcus: Assimilation of Hexadecane in Different Aggregate States

et al. 2022

View full text Add to dashboard Cite

show abstract

“…With regard to the surface tension, the value decreased by up to 46 and 45 mN/m with the B. amyloliquefaciens C11 and S. lavendulae C27 strains, respectively. The values, although high, are in the range of the values of biosurfactants produced by other microorganisms under non-optimized conditions [ 8 , 10 , 13 , 21 ]. The ability to lower the surface tension of aqueous solutions is an important property that characterizes a potent surface-active agent [ 65 ], and according to the literature, a drop in surface tension to under 35 mN/m indicates that the microorganism is an efficient biosurfactant producer [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…These biosurfactants can be applied in a wide variety of productive sectors, such as in the formulation of detergents, cosmetics, body hygiene products, paints and varnishes, agricultural applications, and pharmaceuticals, among others [ 6 ]. An example of an environmental application of biosurfactants is their use in the bioremediation of heavy metals [ 7 ], oil spills [ 8 ], and pesticides [ 9 , 10 ] due to their ability to solubilize hydrophobic compounds through the formation of emulsions that are formed by reducing the surface tension and interfacial tensions between liquids, solids, and gases [ 11 ]. In this process, molecular aggregates (micelles) are formed, which promote mass transfer to the microorganism.…”

Section: Introductionmentioning

confidence: 99%

“…strain isolated from lichens in the Amazon region [ 19 ], Pseudomonas isolated from CHL-enriched soil [ 23 ], Burkholderia sp. isolated from oil-contaminated soil [ 24 ], Bacillus amyloliquefaciens and Bacillus subtilis isolated from contaminated soil [ 20 ], Pseudomonas and Rhodococcus isolated from a pesticide treatment system [ 25 ] and oil spills [ 8 ], Bacillus and Pseudomonas isolated from weathered oil-contaminated soil containing heavy metals [ 26 ], B. subtilis isolated from marine soil sediment [ 27 ], and halotolerant bacteria isolated from karst sinkholes [ 28 ], among others.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biosurfactant Production by Bacillus amyloliquefaciens C11 and Streptomyces lavendulae C27 Isolated from a Biopurification System for Environmental Applications

et al. 2022

View full text Add to dashboard Cite

Biosurfactant-producing bacteria can be found in contaminated environments such as biopurification systems (BPS) for pesticide treatments. A total of 18 isolates were screened to determine their ability to produce extracellular biosurfactants, using olive oil as the main carbon source. Out of the eighteen isolates, two strains (C11 and C27) were selected for biosurfactant production. The emulsification activities of the C11 and C27 strains using sunflower oil was 58.4 and 53.7%, respectively, and 46.6 and 48.0% using olive oil. Using molecular techniques and MALDI-TOF, the strains were identified as Bacillus amyloliquefaciens (C11) and Streptomyces lavendulae (C27). The submerged cultivation of the two selected strains was carried out in a 1 L stirred-tank bioreactor. The maximum biosurfactant production, indicated by the lowest surface tension measurement, was similar (46 and 45 mN/m) for both strains, independent of the fact that the biomass of the B. amyloliquefaciens C11 strain was 50% lower than the biomass of the S. lavendulae C27 strain. The partially purified biosurfactants produced by B. amyloliquefaciens C11 and S. lavendulae C27 were characterized as a lipopeptide and a glycolipid, respectively. These outcomes highlight the potential of the selected biosurfactant-producing microorganisms for improving pesticides’ bioavailability and therefore the degradational efficacy of BPS.

show abstract

“…Strains used in the study were isolated from different sources and characterized as previously described ( Rhodococcus erythropolis X5 25 , Rhodococcus opacus S8 26 , Rhodococcus qingshengii 7B 27 , Rhodococcus qingshengii VT6 28 , Priestia aryabhattai 25 29 , Gordonia amicalis 6-1 30 , Gordonia alkanivorans 135 31,32 , Gordonia polyisoprenivorans 135 33 , Rhodococcus opacus 1CP 34 , Rhodococcus opacus 3D 35 ).…”

Section: Methodsmentioning

confidence: 99%

Ultrafast metaproteomics for quantitative assessment of strain isolates and microbiomes

Kazakova,

Ivanov,

Kusainova

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Microbial proteomics and metaproteomic studies rely heavily on mass spectrometry. Standard liquid chromatography/tandem mass spectrometry-based methods for protein identification and quantitation exhibit lengthy analysis time. Here, we report on the implementation of the previously developed DirectMS1 approach for metaproteomics studies that provides a unique combination of accurate protein quantitation and rapid analysis time. We validated our method using a series of proteome-wide analyses of bacterial samples including strain isolates, mock microbiomes composed of bacteria spiked at known concentrations and human fecal microbiomes. We demonstrated that the developed two-stage search algorithm identifies the bacterial isolate species with an accuracy of 95%, when no prior information on the sample is available. Microbiome composition was resolved at the genus level with the mean difference between the actual and identified microbiome components of 12%. Pearson coefficient of up to 0.97 was achieved in estimates of strain biomass change in mock microbiome samples. By the example of Rhodococcus biodegradation of n-alkanes, phenols and its derivatives, we showed that our approach effectively characterizes changes in the activity of metabolic pathways of strain isolates. For the benchmarking, the changes in biodegradation activity were also assessed using the standard label-free and TMT DDA approaches.

show abstract

Complete Genome Analysis of Rhodococcus opacus S8 Capable of Degrading Alkanes and Producing Biosurfactant Reveals Its Genetic Adaptation for Crude Oil Decomposition

Cited by 8 publications

References 41 publications

Hydrocarbons Biodegradation by Rhodococcus: Assimilation of Hexadecane in Different Aggregate States

Hydrocarbons Biodegradation by Rhodococcus: Assimilation of Hexadecane in Different Aggregate States

Biosurfactant Production by Bacillus amyloliquefaciens C11 and Streptomyces lavendulae C27 Isolated from a Biopurification System for Environmental Applications

Ultrafast metaproteomics for quantitative assessment of strain isolates and microbiomes

Contact Info

Product

Resources

About