Complete genome analysis of Serratia marcescens RSC-14: A plant growth-promoting bacterium that alleviates cadmium stress in host plants

Abstract

Serratia marcescens RSC-14 is a Gram-negative bacterium that was previously isolated from the surface-sterilized roots of the Cd-hyperaccumulator Solanum nigrum. The strain stimulates plant growth and alleviates Cd stress in host plants. To investigate the genetic basis for these traits, the complete genome of RSC-14 was obtained by single-molecule real-time sequencing. The genome of S. marcescens RSC-14 comprised a 5.12-Mbp-long circular chromosome containing 4,593 predicted protein-coding genes, 22 rRNA genes, 88 tRNA genes, and 41 pseudogenes. It contained genes with potential functions in plant growth promotion, including genes involved in indole-3-acetic acid (IAA) biosynthesis, acetoin synthesis, and phosphate solubilization. Moreover, annotation using NCBI and Rapid Annotation using Subsystem Technology identified several genes that encode antioxidant enzymes as well as genes involved in antioxidant production, supporting the observed resistance towards heavy metals, such as Cd. The presence of IAA pathway-related genes and oxidative stress-responsive enzyme genes may explain the plant growth-promoting potential and Cd tolerance, respectively. This is the first report of a complete genome sequence of Cd-tolerant S. marcescens and its plant growth promotion pathway. The whole-genome analysis of this strain clarified the genetic basis underlying its phenotypic and biochemical characteristics, underpinning the beneficial interactions between RSC-14 and plants.

Serratiasp.CP‐13 augments the growth of cadmium (Cd)‐stressedLinum usitatissimumL. by limited Cd uptake, enhanced nutrient acquisition and antioxidative potential

“…CP-13rif, such as phosphate-solubilization, IAA production and ACC-deaminase activity. Our conclusion is supported by a study where S. marcescens RSC-14 was reported to possess the genes involved in biosynthesis of IAA and phosphate solubilization (Khan et al 2017). Our Table 3 Effect of Serratia sp.…”

“…Our conclusion is supported by a study where S. marcescens RSC‐14 was reported to possess the genes involved in biosynthesis of IAA and phosphate solubilization (Khan et al . ). Our findings clearly indicate the efficient role of Serratia sp.…”

“…In the genome of S. marcescens RSC‐14, three genes encoding homologues of the Co/Zn/Cd resistance protein CzcD, as well as the Pb/Cd/Zn/Hg transporting ATPase genes were recently identified (Khan et al . ) and are likely involved in Cd homeostasis and transportation.…”

“…L30 and Klebsiella mobilis CIAM had the ability to accumulate and immobilize soluble Cd 2+ ions in the bacterial mass by binding Cd 2+ into complex forms. In the genome of S. marcescens RSC-14, three genes encoding homologues of the Co/Zn/Cd resistance protein CzcD, as well as the Pb/Cd/Zn/Hg transporting ATPase genes were recently identified (Khan et al 2017) and are likely involved in Cd homeostasis and transportation.…”

Occurrence and molecular identification of the zoysiagrass mite Aceria zoysiae (Acari: Eriophyidae) in turfgrasses in Korea

“…Furthermore, it is difficult to control mite infestation due to the lack of useful management techniques (Lindquist & Amrine 1996). Recent developments in molecular techniques have allowed the precise identification of very small organisms, including eriophyid mites among others (Khan et al 2017;Matsuda et al 2014;Navajas & Navia 2010;Ros & Breeuwer 2007;Shim et al 2016). However, molecular information on eriophyid mites infesting turfgrass is limited.…”