Complete Genome Analysis of Three Live Attenuated Rinderpest Virus Vaccine Strains Derived through Serial Passages in Different Culture Systems

Jeoung, Hye-Young; Lee, Myoung-Heon; Yeh, Jung‐Yong; Lim, Ji-Ae; Lim, Suk-Kyung; Oem, Jae‐Ku; Song, Jae‐Young; Lee, Won‐Ha; Park, Jong‐Hwan; An, Dong-Jun

doi:10.1128/jvi.02362-12

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…All the genomes were 15882 bases long, as previously reported 8,9,[24][25][26][27] , except for 5 samples of goat-adapted vaccine virus (GtVacc), each of which had an extra 6 bases in the long GC-rich 5′ UTR of the F gene ( Fig. 2).…”

Section: Sequence Features In the Completed Genomesmentioning

confidence: 52%

“…For the phylogenetic analysis, duplicate sequences were removed and independent samples of the same isolate replaced with a consensus sequence; 8 Asian RPV genome sequences that have been previously published 24,26,27 or simply deposited in the public sequence databases were included for comparison. The final alignment included 70 genomes, which were analysed by both maximum likelihood (ML) ( Fig.…”

Section: Copy-back Rnas In Virus Samplesmentioning

confidence: 99%

“…Sequences of the same isolate after sequential passage were also not included. In addition to 62 sequences from this study, 8 published full length RPV genomes were included: cattle-passaged RPV/Korea/Fusan-B (AB547189) and the lapinised derivative RPV/Lap/NakamuraIII (AB547190) 24 ; three further lapinised/avianised derivatives of Nakamura III namely RPV/Lap/L72 (JN234008), RPV/Lap/LA77 (JN234009) and RPV/Lap/LA96 (JN234010) 26 ; an additional lapinised/avianised strain, RPV/ Lap/LATC06 (GU168576) 25 ; the current lapinised/avianised RPV vaccine kept in Japan, RPV/Lap/LA-AKO (LC057619) 27 ; an unpublished sequence of a lapinised strain from the University of Tokyo, RPV/Lap/Lv (LC168749). We did not include our previously published sequences for the RBOK vaccine strain and the Kabete 'O' wildtype virus 8,9 as these viruses were included in the set of genome sequences determined in this study and those sequences would be more reliable than those originally determined from cDNA libraries.…”

Section: Rapid Amplification Of Sequence Ends (Race)mentioning

confidence: 99%

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Full genome sequencing of archived wild type and vaccine rinderpest virus isolates prior to their destruction

King

Rajko-Nenow

Ropiak

et al. 2020

Sci Rep

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When rinderpest virus (RPV) was declared eradicated in 2011, the only remaining samples of this once much-feared livestock virus were those held in various laboratories. in order to allow the destruction of our institute's stocks of RpV while maintaining the ability to recover the various viruses if ever required, we have determined the full genome sequence of all our distinct samples of RPV, including 51 wild type viruses and examples of three different types of vaccine strain. Examination of the sequences of these virus isolates has shown that the African isolates form a single disparate clade, rather than two separate clades, which is more in accord with the known history of the virus in Africa. We have also identified two groups of goat-passaged viruses which have acquired an extra 6 bases in the long untranslated region between the M and f protein coding sequences, and shown that, for more than half the genomes sequenced, translation of the f protein requires translational frameshift or non-standard translation initiation. curiously, the clade containing the lapinised vaccine viruses that were developed originally in Korea appears to be more similar to the known African viruses than to any other Asian viruses.Rinderpest (RP) was one of the most severe diseases of cattle ever recorded, with high morbidity rates, and mortality rates of 80% to 90% in naïve populations. The disease was declared eradicated in 2011 1 , thus becoming the second viral disease, after smallpox, to be eradicated, with global benefits estimated to be in the billions of dollars 2 . The RP virus (RPV) itself has not been entirely eliminated, with a number of laboratories known to have samples of wild type RPV. Accidental release of RPV from such a laboratory is thought to be the most likely pathway by which the virus might re-enter the environment 3,4 , although it might also be deliberately released as an act of sabotage or bioterrorism. The member states of the World Organisation for Animal Health (OIE), and the Food and Agricultural Organisation of the United Nations (FAO) agreed to restrict all work with the virus and to allow the storage of the virus only in highly secure Rinderpest Holding Facilities (RHFs) that have been inspected and approved jointly by OIE and FAO.The FAO-OIE RHF in the UK is the Pirbright Institute which, as the Institute for Animal Health, and before that the Animal Virus Research Institute, was a centre for research on RPV since the 1960s. The institute developed the monoclonal antibody-based competition ELISA (cELISA) used extensively for surveillance 5 , as well as the most widely used RT-PCR assay for RPV 6 and the concept of different geographic lineages of the virus based on the sequence of the product from the RT-PCR 7 . The first RPV genome sequences were determined at Pirbright 8,9 and the system for recovering RPV from a cDNA copy of the genome was developed there 10 . Because of this history, and its links to many of the countries where RPV was still endemic in the latter half of the 20 th century, t...

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Section: Sequence Features In the Completed Genomesmentioning

confidence: 52%

Section: Copy-back Rnas In Virus Samplesmentioning

confidence: 99%

Section: Rapid Amplification Of Sequence Ends (Race)mentioning

confidence: 99%

See 1 more Smart Citation

Full genome sequencing of archived wild type and vaccine rinderpest virus isolates prior to their destruction

King

Rajko-Nenow

Ropiak

et al. 2020

Sci Rep

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Novel cetacean morbillivirus in a rare Fraser’s dolphin (Lagenodelphis hosei) stranding from Maui, Hawai‘i

West

Silva‐Krott

Landrau‐Giovannetti

et al. 2021

Sci Rep

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Cetacean morbillivirus (CeMV) is a global threat to cetaceans. We report a novel morbillivirus from a Fraser’s dolphin (Lagenodelphis hosei) that stranded in Maui, Hawaii in 2018 that is dissimilar to the beaked whale morbillivirus previously identified from Hawaii and to other CeMV strains. Histopathological findings included intranuclear inclusions in bile duct epithelium, lymphoid depletion, rare syncytial cells and non-suppurative meningitis. Cerebellum and lung tissue homogenates were inoculated onto Vero.DogSLAMtag cells for virus isolation and cytopathic effects were observed, resulting in the formation of multinucleated giant cells (i.e., syncytia). Transmission electron microscopy of infected cell cultures also revealed syncytial cells with intracytoplasmic and intranuclear inclusions of viral nucleocapsids, consistent with the ultrastructure of a morbillivirus. Samples of the cerebellum, lung, liver, spleen and lymph nodes were positive for morbillivirus using a reverse transcription-polymerase chain reaction. The resulting 559 bp L gene sequence had the highest nucleotide identity (77.3%) to porpoise morbillivirus from Northern Ireland and the Netherlands. The resulting 248 bp P gene had the highest nucleotide identity to porpoise morbillivirus in Northern Ireland and the Netherlands and to a stranded Guiana dolphin (Sotalia guianensis) in Brazil (66.9%). As Fraser’s dolphins are a pelagic species that infrequently strand, a novel strain of CeMV may be circulating in the central Pacific that could have additional population impacts through transmission to other small island-associated cetacean species.

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Complete Genome Analysis of Three Live Attenuated Rinderpest Virus Vaccine Strains Derived through Serial Passages in Different Culture Systems

Cited by 2 publications

References 5 publications

Full genome sequencing of archived wild type and vaccine rinderpest virus isolates prior to their destruction

Full genome sequencing of archived wild type and vaccine rinderpest virus isolates prior to their destruction

Novel cetacean morbillivirus in a rare Fraser’s dolphin (Lagenodelphis hosei) stranding from Maui, Hawai‘i

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