Complete genome of Pseudomonas sp. strain L10.10, a psychrotolerant biofertilizer that could promote plant growth

See-Too, Wah-Seng; Lim, Yan-Lue; Ee, Robson; Convey, Peter; Pearce, David A.; Yin, Wai‐Fong; Chan, Kok‐Gan

doi:10.1016/j.jbiotec.2016.02.017

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…Pseudomonas , See‐Too et al . ()) and numerous studies have reported bacterial genera, such as Polarmonas , Flavobacteria and Sphingobacteria , living in close association with Chloromonas and Chlamydomonadaceae (Hoham & Duval, ; Komárek & Nedbalová, ; Hisakawa et al ., ; Lutz et al ., , , ; Hamilton & Havig, ). There were also a large number of OTUs that could not be assigned to a genus or species, implying that the communities in the Antarctic snow packs are yet to be fully identified, characterised and incorporated into public databases such as Silva (Quast et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Snow algae communities in Antarctica: metabolic and taxonomic composition

et al. 2019

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Summary Snow algae are found in snowfields across cold regions of the planet, forming highly visible red and green patches below and on the snow surface. In Antarctica, they contribute significantly to terrestrial net primary productivity due to the paucity of land plants, but our knowledge of these communities is limited. Here we provide the first description of the metabolic and species diversity of green and red snow algae communities from four locations in Ryder Bay (Adelaide Island, 68°S), Antarctic Peninsula. During the 2015 austral summer season, we collected samples to measure the metabolic composition of snow algae communities and determined the species composition of these communities using metabarcoding. Green communities were protein‐rich, had a high chlorophyll content and contained many metabolites associated with nitrogen and amino acid metabolism. Red communities had a higher carotenoid content and contained more metabolites associated with carbohydrate and fatty acid metabolism. Chloromonas , Chlamydomonas and Chlorella were found in green blooms but only Chloromonas was detected in red blooms. Both communities also contained bacteria, protists and fungi. These data show the complexity and variation within snow algae communities in Antarctica and provide initial insights into the contribution they make to ecosystem functioning.

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Section: Discussionmentioning

confidence: 99%

Snow algae communities in Antarctica: metabolic and taxonomic composition

et al. 2019

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“…The complete genome of strain L10.10 T has been previously sequenced, enabling the discovery of various genes encoding for plant growth promoting properties and plant disease prevention attributes [45]. The draft genomes of strains A4R1.5 and A4R1.12 were obtained in this study in order to perform whole genome sequence analysis.…”

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confidence: 99%

Pseudomonas versuta sp. nov., isolated from Antarctic soil

See-Too

Salazar

et al. 2017

Systematic and Applied Microbiology

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In this study we analysed three bacterial strains coded L10.10, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring in Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993. Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95% with respect to the closest related species of Pseudomonas, confirming its phylogenetic affiliation. The ANI values were less than 86% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/ C16:1 ω6c in summed feature 3 and C18:1 ω7c / C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. Growth occurs at 4-30°C, and at pH 4.0-10. The DNA G+C content is 58.2-58.3mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10 (LMG 29628, DSM 101070).

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“…Lz4W, Pseudomonas sp. L.10.10, and P. deceptionensis LMG25555; [50,52,54]. The P. aeruginosa strains were also the most divergent and ancestral branch among the set.…”

Section: Genetic Relationships and Pan-genome Analysismentioning

confidence: 95%

“…GC01 also closely resembles Pseudomonas sp. L.10.10 [52], with a 91% sequence identity, followed by an 85% sequence identity to P. fragi DBC [53] and two strains of P. deceptionensis LMG25555 and DSM26521 [54,55]. Interestingly, the strains with the higher similarity to Pseudomonas sp.…”

Section: Genetic Relationships and Pan-genome Analysismentioning

confidence: 98%

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

Gallardo-Benavente

Campo-Giraldo

Castro‐Severyn

et al. 2021

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Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains.

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Complete genome of Pseudomonas sp. strain L10.10, a psychrotolerant biofertilizer that could promote plant growth

Cited by 12 publications

References 12 publications

Snow algae communities in Antarctica: metabolic and taxonomic composition

Snow algae communities in Antarctica: metabolic and taxonomic composition

Pseudomonas versuta sp. nov., isolated from Antarctic soil

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

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