Complete Genome Sequence Data of Rare Actinomycetes Strain <i>Saccharothrix texasensis</i> 6-C, a Biological Control Agent for Potato Late Blight

Feng, Shun; Jian, Yongfei; Liang, Jin; Tang, Shicai; Li, Zhengguo

doi:10.1094/mpmi-10-20-0300-a

Cited by 7 publications

(9 citation statements)

References 23 publications

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“…Almost all active strains belonged to the Streptomyces genus, while isolates affiliated with the species N. flavescens and S. espanaensis showed very little activity against P. infestans mycelial growth. This contrasts with a recent study reporting anti- Phytophthora activity of a Saccharothrix texasensis strain isolated from the potato rhizosphere ( Feng et al, 2021 ). Moreover, all strains belonging to the species N. dassonvillei also strongly inhibited P. infestans mycelial growth, but their opportunistic human pathogen status precludes their use as biocontrol agents and they were therefore not investigated any further in the present study.…”

Section: Discussioncontrasting

confidence: 97%

“…In view of their above-mentioned prolific production of bioactive metabolites, Actinomycetes appear as promising candidates to contribute to sustainable solutions for late blight control. Indeed, earlier studies have reported inhibition of P. infestans mycelial growth by different Streptomyces strains or their metabolites (Sinha et al, 2014;Bermejo et al, 2020;Feng et al, 2021). However, in vitro inhibition activities on P. infestans are no warranty for the ability of the strains to restrict disease progress on plant material such as leaf discs or full plants, which in turn do not warrant efficacy in the field (Guyer et al, 2015;Hashemi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Antagonistic Potential of Actinomycete Strains Isolated From Sudan’s Soils Against Phytophthora infestans

et al. 2022

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Soil microorganisms play crucial roles in soil fertility, e.g., through decomposing organic matter, cycling nutrients or through beneficial interactions with plants. Actinomycetes are a major component of soil inhabitants; they are prolific producers of specialized metabolites, among which many antibiotics. Here we report the isolation and characterization of 175 Actinomycetes from rhizosphere and bulk soil samples collected in 18 locations in Sudan. We evaluated the strains’ metabolic potential for plant protection by testing their ability to inhibit the mycelial growth of the oomycete Phytophthora infestans, which is one of the most devastating plant pathogens worldwide. Most strains significantly reduced the oomycete’s growth in direct confrontational in vitro assays. A significant proportion of the tested strains (15%) were able to inhibit P. infestans to more than 80%, 23% to 50%–80%, while the remaining 62% had inhibition percentages lesser than 50%. Different morphologies of P. infestans mycelial growth and sporangia formation were observed upon co-inoculation with some of the Actinomycetes isolates, such as the production of fewer, thinner hyphae without sporangia leading to a faint growth morphology, or on the contrary, of clusters of thick-walled hyphae leading to a bushy, or “frozen” morphology. These morphologies were caused by strains differing in activity levels but phylogenetically closely related with each other. To evaluate whether the isolated Actinomycetes could also inhibit the pathogen’s growth in planta, the most active strains were tested for their ability to restrict disease progress in leaf disc and full plant assays. Five of the active strains showed highly significant protection of potato leaves against the pathogen in leaf disc assays, as well as substantial reduction of disease progress in full plants assays. Using cell-free filtrates instead of the bacterial spores also led to full protection against disease on leaf discs, which highlights the strong crop protective potential of the secreted metabolites that could be applied as leaf spray. This study demonstrates the strong anti-oomycete activity of soil- and rhizosphere-borne Actinomycetes and highlights their significant potential for the development of sustainable solutions based on either cell suspensions or cell-free filtrates to safeguard potatoes from their most damaging pathogen.

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Section: Discussioncontrasting

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Evaluating the Antagonistic Potential of Actinomycete Strains Isolated From Sudan’s Soils Against Phytophthora infestans

et al. 2022

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“…( Khan et al, 2018 ). Biological control of plant diseases has always been a hot topic in the agricultural field, and good results have been achieved ( Howell, 2003 ; Alabouvette et al, 2006 ; Feng et al, 2021 ). The rhizobacterium B. subtilis is considered one of the most widely used and well-studied biocontrol organisms, and 4–5% of its genome is responsible for the synthesis of antibiotics such as the cyclic lipopeptides (LPs) surfactin, iturin, and fengycin ( Stein, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Biocontrol Mechanism of Bacillus subtilis C3 Against Bulb Rot Disease in Fritillaria taipaiensis P.Y.Li

Yang

et al. 2021

Front. Microbiol.

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Bulb rot disease has become one of the main diseases that seriously affects the yield and quality of Fritillaria taipaiensis P.Y.Li (F. taipaiensis). In this study, F. taipaiensis was used as the research object to explore the effect and mechanism of Bacillus subtilis C3 in preventing and curing bulb rot. Through isolation and verification of the pathogenic fungi, we determined for the first time that the pathogenic fungus that causes bulb rot in F. taipaiensis is Fusarium oxysporum. The results of the study showed that B. subtilis C3 inhibits the growth of pathogenic fungi, and the inhibition rate is as high as 60%. In the inhibition mechanism, strain C3 inhibits the conidiogenesis of pathogenic fungi and destroys the cell structure of its hyphae, causing protoplast exudation, chromatin concentration, DNA fragmentation, and ultimately cell death. Among the secondary metabolites of C3, antimicrobial proteins and main active components (paeonol, ethyl palmitate, and oxalic acid) inhibited the growth of F. oxysporum. The molecular weight of the antibacterial protein with the highest inhibition rate was approximately 50 kD. The results of a field experiment on the Taibai Mountain F. taipaiensis planting base showed that after the application of strain C3, the incidence of bulb rot in Fritillaria was reduced by 18.44%, and the ratio of bacteria to fungi in the soil increased to 8.21, which verified the control effect of C3 on Fritillaria bulb rot disease. This study provides a theoretical basis for the use of B. subtilis C3 to prevent and control bulb rot in Fritillaria.

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“…Fungicidal activity was also observed with ethyl-acetate extracts from strains belonging to the fungal genus Chaetomium [ 25 ]. Living microorganisms, such as bacterial strains of Streptomyces , Pseudomonas , Saccharothrix, Bacillus , and Nocardiopsis , are also able to act as biological control agents against PLB [ 26 , 27 , 28 , 29 ]. However, these molecules and strains have not been tested under field conditions.…”

Section: Discussionmentioning

confidence: 99%

A New Biocontrol Tool to Fight Potato Late Blight Based on Willaertia magna C2c Maky Lysate

Troussieux¹,

Gilgen²,

Souche³

2022

Plants

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Potato late blight (PLB) is one of the most destructive disease affecting potatoes. Late blight control relies almost exclusively on the use of chemical pesticides, including copper products, which are efficient but controversial due to their environmental toxicity. Societal pressure and the quest for more sustainable agriculture reinforce the need for natural plant protection products. To respond to this demand, we tested the lysate of the amoeba Willaertia magna C2c Maky on PLB. This active substance exhibits plant protection properties against grape downy mildew thanks to a dual mode of action (plant elicitor and antifungal direct effect). We hypothesized that this active substance might also have an effect against other diseases caused by oomycetes on other crops, such as potato. In vitro, in planta, and in-field studies were conducted. The collected data demonstrate that the lysate of the amoeba Willaertia magna C2c Maky is able to elicit potato defenses, and direct fungicidal activity against Phytophtora infestans was observed. Proof of efficacy was first obtained in greenhouse, with up to 80% disease reduction, and confirmed in field trials. Formulated products provided up to 77% protection in field in the case of low infestation (28%) and up to 49% protection when the untreated plants were 100% destroyed. Willaertia magna C2c Maky was also able to significantly increase yield by up to 30% in field trials.

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Complete Genome Sequence Data of Rare Actinomycetes Strain Saccharothrix texasensis 6-C, a Biological Control Agent for Potato Late Blight

Cited by 7 publications

References 23 publications

Evaluating the Antagonistic Potential of Actinomycete Strains Isolated From Sudan’s Soils Against Phytophthora infestans

Evaluating the Antagonistic Potential of Actinomycete Strains Isolated From Sudan’s Soils Against Phytophthora infestans

Biocontrol Mechanism of Bacillus subtilis C3 Against Bulb Rot Disease in Fritillaria taipaiensis P.Y.Li

A New Biocontrol Tool to Fight Potato Late Blight Based on Willaertia magna C2c Maky Lysate

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