Complete Genome Sequence of a Radioresistant Bacterial Strain, Deinococcus grandis ATCC 43672

Shibai, Atsushi; Satoh, Katsuya; Kawada, Manabu; Kotani, Hazuki; Narumi, Issay; Furusawa, Chikara

doi:10.1128/mra.01226-19

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…Like in D. deserti, the imuY, imuB-C and dnaE2 genes of D. peraridilitoris are located in a probable operon with lexA upstream of imuY (Figure 1). Our recent BLAST searches and sequence analyses revealed that this lexA-imuY-imuB-C-dnaE2 operon is present in at least 20 other Deinococcus species, including Deinococcus ficus [60], Deinococcus grandis [61], Deinococcus koreensis [62] and Deinococcus marmoris [63] for which the genome sequence has been published (Table 1; Supplementary Table S1). Remarkably, several of these species, including D ficus, D grandis and D. koreensis, have two different lexA-imuY-imuB-C-dnaE2 operons (gene products of one operon are not identical to the counterparts of the second operon).…”

Section: Presence Of Imuy-imub-c-dnae2 Mutagenesis Cassette In Many Deinococcus Speciesmentioning

confidence: 99%

Coexistence of SOS-Dependent and SOS-Independent Regulation of DNA Repair Genes in Radiation-Resistant Deinococcus Bacteria

Blanchard

Groot

2021

Cells

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Deinococcus bacteria are extremely resistant to radiation and able to repair a shattered genome in an essentially error-free manner after exposure to high doses of radiation or prolonged desiccation. An efficient, SOS-independent response mechanism to induce various DNA repair genes such as recA is essential for radiation resistance. This pathway, called radiation/desiccation response, is controlled by metallopeptidase IrrE and repressor DdrO that are highly conserved in Deinococcus. Among various Deinococcus species, Deinococcus radiodurans has been studied most extensively. Its genome encodes classical DNA repair proteins for error-free repair but no error-prone translesion DNA polymerases, which may suggest that absence of mutagenic lesion bypass is crucial for error-free repair of massive DNA damage. However, many other radiation-resistant Deinococcus species do possess translesion polymerases, and radiation-induced mutagenesis has been demonstrated. At least dozens of Deinococcus species contain a mutagenesis cassette, and some even two cassettes, encoding error-prone translesion polymerase DnaE2 and two other proteins, ImuY and ImuB-C, that are probable accessory factors required for DnaE2 activity. Expression of this mutagenesis cassette is under control of the SOS regulators RecA and LexA. In this paper, we review both the RecA/LexA-controlled mutagenesis and the IrrE/DdrO-controlled radiation/desiccation response in Deinococcus.

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Section: Presence Of Imuy-imub-c-dnae2 Mutagenesis Cassette In Many Deinococcus Speciesmentioning

confidence: 99%

Coexistence of SOS-Dependent and SOS-Independent Regulation of DNA Repair Genes in Radiation-Resistant Deinococcus Bacteria

Blanchard

Groot

2021

Cells

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“…Plasmid pDEGR-PL was deleted via cultivation at 40°C, the upper limit of the permissible temperature, whereas pDEGR-3 was stable against heat treatment. Plasmid pDEGR-PL was undetected via whole genome sequencing in a previous study ( Shibai et al, 2019 ). As the largest coding DNA sequence of the undetected circular contig was similar to that of the phage tail protein, this circular DNA was assumed to be a mobile genetic factor and thus lost prior to whole-genome sequencing.…”

Section: Discussionmentioning

confidence: 98%

“…We previously analyzed the draft and whole genome sequences of D. grandis ( Satoh et al, 2016 ; Shibai et al, 2019 ); both are available in public genome databases (GenBank Assembly ID: GCA_001485435.1 and GCA_009177165.1). Table 2 summarizes results of the two analyses.…”

Section: Discussionmentioning

confidence: 99%

Developing a new host-vector system for Deinococcus grandis

Sakai,

Shimosaka,

Katsumata

et al. 2024

Front. Microbiol.

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Deinococcus spp. are known for their radiation resistance, toxic compound removal, and production of valuable substances. Therefore, developing gene expression systems for Deinococcus spp. is crucial in advancing genetic engineering applications. To date, plasmid vectors that express foreign genes in D. radiodurans and D. geothermalis have been limited to plasmid pI3 and its derivatives. In contrast, plasmid vectors that express foreign genes in D. grandis include plasmid pZT23 and its derivatives. In this study, we developed a new system for the stable introduction and retention of expression plasmids for D. grandis. Two cryptic plasmids were removed from the wild-type strain to generate the TY3 strain. We then constructed a shuttle vector plasmid, pGRC5, containing the replication initiation region of the smallest cryptic plasmid, pDEGR-3, replication initiation region of the E. coli vector, pACYC184, and an antibiotic resistance gene. We introduced pGRC5, pZT23-derived plasmid pZT29H, and pI3-derived plasmid pRADN8 into strain TY3, and found their coexistence in D. grandis cells. The quantitative PCR assay results found that pGRC5, pZT29H, and pRADN8 had relative copy numbers of 11, 26, and 5 per genome, respectively. Furthermore, we developed a new plasmid in which the luciferase gene was controlled by the promoter region, which contained radiation-desiccation response operator sequences for D. grandis DdrO, a stress response regulon repressor in D. grandis, hence inducing gene expression via ultraviolet-C light irradiation. These plasmids are expected to facilitate the removal and production of toxic and valuable substances, in D. grandis, respectively, particularly of those involving multiple genes.

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“…Bacterial extracts. Some bacterial species are resistant to radiation (122). A recombinant polypeptide from Salmonella flagellin (CBLB502) exhibited high radioprotective efficacy in mice and primates (92).…”

Section: Emerging Radioprotectorsmentioning

confidence: 99%

Radioprotective countermeasures for radiation injury (Review)

Liu

Liang

et al. 2023

Mol Med Rep

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A series of physiological and pathological changes occur after radiotherapy and accidental exposure to ionizing radiation (IR). These changes cause serious damage to human tissues and can lead to death. Radioprotective countermeasures are radioprotective agents that prevent and reduce IR injury or have therapeutic effects. Based on a good understanding of radiobiology, a number of protective agents have achieved positive results in early clinical trials. The present review grouped known radioprotective agents according to biochemical categories and potential clinical use, and reviewed radiation countermeasures, i.e., radioprotectors, radiation mitigators and radiotherapeutic agents, with an emphasis on their current status and research progress. The aim of the present review is to facilitate the selection and application of suitable radioprotectors for clinicians and researchers, to prevent or reduce IR injury.

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Complete Genome Sequence of a Radioresistant Bacterial Strain, Deinococcus grandis ATCC 43672

Cited by 5 publications

References 9 publications

Coexistence of SOS-Dependent and SOS-Independent Regulation of DNA Repair Genes in Radiation-Resistant Deinococcus Bacteria

Coexistence of SOS-Dependent and SOS-Independent Regulation of DNA Repair Genes in Radiation-Resistant Deinococcus Bacteria

Developing a new host-vector system for Deinococcus grandis

Radioprotective countermeasures for radiation injury (Review)

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