Complete genome sequence of a potential foot-and-mouth disease virus serotype O vaccine strain from Bangladesh

Amin, Al; Ali, Md. Ramjan; Alam, Arshad; Siddique, Mohammad Anwar; Rahaman, Mizanur; Sultana, Munawar; Hossain, M. Anwar

doi:10.1007/s00705-020-04711-6

Cited by 2 publications

(5 citation statements)

References 14 publications

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“…In-depth genomic analysis showed the presence of ten mutations in the major antigenic sites of the recently published vaccine candidate strain BAN/TA/Dh-301/2016 (MK088170.1), demonstrating significant evolutionary distance from the previously established serotype O vaccine strain, O/India/R2/75 (Accession no. AF204276) [ 49 ]. Additionally, a homology analysis of the recently disclosed serotype-A vaccine strain, A/BAN/CH/Sa-304/2016 (MK088171.1) indicated a 91 % resemblance to the vaccine strain, IND40/00 now in use (Accession no.…”

Section: Discussionmentioning

confidence: 99%

“…For assessment of virus neutralization efficiency of the chimaeric proteins, two recently reported vaccine FMDV strains for both serotype O (BAN/TA/Dh-301/2016) and serotype A (BAN/CH/Sa-304/2016) have been collected from the repository of MGBL and revived using BHK-21 (ATCC no#BHK-21[C-13]-CCL-10) cell line [ 31 32 ]. The infectivity and tissue culture infective dose 50 (TCID 50 ) were determined for each strain.…”

Section: Methodsmentioning

confidence: 99%

“…The control groups X and Y were inoculated with inactivated monovalent FMDV vaccine types O and A. These vaccines were prepared from the two recently reported vaccine strains BAN/TA/Dh-301/2016 (serotype-O) and BAN/CH/Sa-304/2016 (serotype-A) [ 31 32 ]. The control group-Z was inoculated with only 1.0 ml PBS, mixed with an adjuvant.…”

Section: Methodsmentioning

confidence: 99%

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Development of recombinant proteins for vaccine candidates against serotypes O and A of Foot-and-Mouth Disease virus in Bangladesh

Akter,

Rahman,

Islam

et al. 2024

Access Microbiology

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Frequent vaccine failure leading to recurrent outbreaks of Foot-and-Mouth Disease (FMD) in livestock populations necessitates the development of a customizable vaccine platform comprising potential antigenic determinants of circulating lineages of FMD viruses. Artificially designed, chimaeric protein-based recombinant vaccines are novel approaches to combat the phylogenetically diverse FMD Virus (FMDV) strains. Among seven recognized serotypes, only serotypes O and A are dominantly circulating in Bangladesh and neighbouring countries of Asia, where transboundary transmission, recurrent outbreaks and emergence of novel lineages of FMDV are highly prevalent. The objective of this study was to develop multi-epitope recombinant proteins, procuring immunogenicity against circulating diverse genotypes of FMDV serotypes O and A. Two chimaeric proteins, named B1 (41.0 kDa) and B3 (39.3 kDa), have been designed to incorporate potential B-cell and T-cell epitopes selected from multiple FMDV strains, including previously reported and newly emerged sub-lineages. After expression, characterization and immunization of guinea pigs with a considerable antigen load of B1 and B3 followed by serological assays revealed the significant protective immunogenicity, developed from the higher (100 µg) doses of both antigens, against most of the currently prevalent serotype O and A strains of FMDV. The efficient expression, antigenic stability, and multivalent immunogenic potency of the chimaeric proteins strongly indicate their credibility as novel vaccine candidates for existing serotypes O and A of FMDV in Bangladesh and surrounding territories.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Development of recombinant proteins for vaccine candidates against serotypes O and A of Foot-and-Mouth Disease virus in Bangladesh

Akter,

Rahman,

Islam

et al. 2024

Access Microbiology

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“…Recently, Al Amin et al reported the development of trivalent inactivated FMD vaccine composed of newly emerged global strains of each serotype [20][21][22][23]. Several limitations of inactivated whole virus vaccine pose potential threats towards its acceptability.…”

Section: Introductionmentioning

confidence: 99%

“…The control groups X and Y were inoculated with inactivated monovalent FMDV vaccine types O and A. These vaccines were prepared from the two recently reported vaccine strains BAN/TA/Dh-301/2016 (serotype-O) and BAN/CH/Sa-304/2016 (serotype-A)[21,23]. The control group-Z were inoculated with only 1.0 ml PBS, mixed with an adjuvant.…”

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confidence: 99%

Immuno-Efficacy of Multi-Epitope Chimeric Peptides Against Foot and Mouth Disease Virus: Potential Vaccine Candidates for Newly Emerged Serotype O and A

Akter¹,

Rahman²,

Islam³

et al. 2021

Preprint

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Artificially designed, chimeric peptide-based recombinant vaccines are novel approaches to combat the phylogenetically diverse Foot and Mouth Disease (FMD) Virus (FMDV) strains. Among seven distinct serotypes, only serotype O and A are dominantly circulating in Bangladesh and neighbouring countries of Asia, where transboundary transmission, recurrent outbreaks and emergence of novel lineages FMDV are highly prevalent. The objective of this study was to develop multi-epitope recombinant peptides, procuring immunogenicity against circulating diverse subtypes of FMDV serotype O and A. Two chimeric peptides, named B1 (41.0 kDa) and B3 (39.3 kDa), have been designed to incorporate potential B-cell and T-cell epitopes selected from multiple FMDV strains, including previously reported and newly emerged sub-lineages. After expression, characterization and immunization of guineapigs with considerable antigen load of B1 and B3 followed by the serological assays revealed the significant protective immunogenicity, developed from the higher (100 µg) doses of both antigens, against most of the currently prevalent serotype O and A strains of FMDV. The efficient expression, antigenic stability, and multivalent immunogenic potency of the chimeric peptides strongly indicate their credibility as novel vaccine candidates for FMDV serotypes O and A circulating in Bangladesh and surrounding territories.

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Complete genome sequence of a potential foot-and-mouth disease virus serotype O vaccine strain from Bangladesh

Cited by 2 publications

References 14 publications

Development of recombinant proteins for vaccine candidates against serotypes O and A of Foot-and-Mouth Disease virus in Bangladesh

Development of recombinant proteins for vaccine candidates against serotypes O and A of Foot-and-Mouth Disease virus in Bangladesh

Immuno-Efficacy of Multi-Epitope Chimeric Peptides Against Foot and Mouth Disease Virus: Potential Vaccine Candidates for Newly Emerged Serotype O and A

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