Complete genome sequence of a novel secovirid infecting cassava in the Americas

Leiva, Ana Marı́a; Jimenez, Jenyfer; Sandoval, Héctor; Perez, Shirley; Cuellar, Wilmer J.

doi:10.1007/s00705-021-05325-2

Cited by 14 publications

(8 citation statements)

References 13 publications

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“… 2020 , Leiva et al . 2022 ), as well as in bacteriophages and giant DNA viruses (Kiljunen et al . 2005 , Deeg, Chow and Suttle 2018 , Sun and Ku 2021 ).…”

Section: Diversity and Evolution Of Non-core Modulesmentioning

confidence: 99%

Proteome expansion in thePotyviridaeevolutionary radiation

Pasin

Daròs

Tzanetakis

2022

FEMS Microbiology Reviews

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Potyviridae, the largest family of known RNA viruses (realm Riboviria), belongs to the picorna-like supergroup and has important agricultural and ecological impacts. Potyvirid genomes are translated into polyproteins, which are in turn hydrolyzed to release mature products. Recent sequencing efforts revealed an unprecedented number of potyvirids with a rich variability in gene content and genomic layouts. Here we review the heterogeneity of non-core modules that expand the structural and functional diversity of the potyvirid proteomes. We provide a family-wide classification of P1 proteinases into the functional Types A and B, and discuss pretty interesting sweet potato potyviral ORF (PISPO), putative zinc fingers, and alkylation B (AlkB) – non-core modules found within P1 cistrons. The atypical modules inosine triphosphate pyrophosphatase (ITPase/HAM1), as well as the pseudo tobacco mosaic virus-like coat protein (TMV-like CP) are discussed alongside homologs of unrelated virus taxa. Family-wide abundance of the multitasking helper component proteinase (HC-pro) is revised. Functional connections between non-core modules are highlighted to support host niche adaptation and immune evasion as main drivers of the Potyviridae evolutionary radiation. Potential biotechnological and synthetic biology applications of potyvirid leader proteinases and non-core modules are finally explored.

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“… 2020 , Leiva et al . 2022 ), as well as in bacteriophages and giant DNA viruses (Kiljunen et al . 2005 , Deeg, Chow and Suttle 2018 , Sun and Ku 2021 ).…”

Section: Diversity and Evolution Of Non-core Modulesmentioning

confidence: 99%

Proteome expansion in thePotyviridaeevolutionary radiation

Pasin

Daròs

Tzanetakis

2022

FEMS Microbiology Reviews

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“…As it has been recently reported that plant viruses infecting Euphorbiaceae and especially Manihot esculenta (commonly known as Cassava) possess a homologue of ITPA (James et al, 2021; Leiva et al, 2022), we were curious whether ITP and IDP concentrations change during infection with such a virus. We chose to investigate this question in N. benthamiana because Arabidopsis is not a known host of viruses encoding ITPA.…”

Section: Resultsmentioning

confidence: 99%

An inosine triphosphate pyrophosphatase safeguards plant nucleic acids from aberrant purine nucleotides

Straube

Rinne

et al. 2022

Preprint

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Inosine is deaminated adenosine. Inosine is enzymatically introduced in some plant tRNAs but not in other RNAs or DNA. Nonetheless, our data show that RNA and DNA from Arabidopsis thaliana contain (deoxy)inosine, probably derived from non-enzymatic adenosine deamination in nucleic acids and usage of (deoxy)inosine triphosphate (ITP / dITP) during nucleic acid synthesis. We identified a plant INOSINE TRIPHOSPHATE PYROPHOSPHATASE (ITPA) which is conserved in many organisms. Arabidopsis ITPA dephosphorylates deaminated nucleoside di- and triphosphates to their respective monophosphates. ITPA mutation causes inosine di- and triphosphate accumulation in vivo and an elevated (deoxy)inosine content in DNA and RNA. Cadmium-induced oxidative stress, known to foster deamination, leads to more ITP in the wildtype and especially in itpa mutants suggesting that ITP originates from ATP deamination. By contrast, erroneous IMP phosphorylation by AMP kinases seems not to be a major ITP source in vivo although these enzymes phosphorylate IMP in vitro. Mutation of ITPA causes salicylic acid (SA) accumulation, early senescence and upregulation of transcripts associated with immunity and senescence. ITPA is part of a molecular protection system, preventing accumulation of (d)ITP, its usage for nucleic acid synthesis, and probably nucleic acid stress leading to SA accumulation, stress gene induction and early senescence.

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“…After its first report in the south of Colombia, in the 1970s, the characteristic root symptoms of the disease have been reported in all the main Colombian regions where cassava is grown [ 1 , 11 , 15 , 26 ]. Here, it is worth mentioning that during surveys in the North Coast of Colombia in the early 1990s, root symptoms of CFSD were also associated with leaf mosaic symptoms, probably due to Secundina being at the time a popular genotype grown in this region.…”

Section: Symptoms Transmission and Geographical Distributionmentioning

confidence: 99%

“…As mentioned above, isolation by mechanical inoculation is not possible for most of these pathogens, and its common occurrence in mixed infections makes it harder to evaluate its individual or combined effect on cassava. Recently, Leiva et al [ 26 ] reported the occurrence of CsTLV in mixed infections with phytoplasma in fields with a high incidence of CFSD in the Department of Casanare (eastern Colombia). Several years of experience working with these pathogens have validated mechanical isolation in Nicotiana benthamiana plants, only for CsCMV and CsVX, which are not associated with root symptoms of CFSD.…”

Section: Cfsd-associated Pathogens and Molecular Diagnosticsmentioning

confidence: 99%

Cassava Frogskin Disease: Current Knowledge on a Re-Emerging Disease in the Americas

Pardo

Álvarez

López-Lavalle

et al. 2022

Plants

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Cassava frogskin disease (CFSD) is a graft-transmissible disease of cassava reported for the first time in the 1970s, in Colombia. The disease is characterized by the formation of longitudinal lip-like fissures on the peel of the cassava storage roots and a progressive reduction in fresh weight and starch content. Since its first report, different pathogens have been identified in CFSD-affected plants and improved sequencing technologies have unraveled complex mixed infections building up in plants with severe root symptoms. The re-emergence of the disease in Colombia during 2019–2020 is again threatening the food security of low-income farmers and the growing local cassava starch industry. Here, we review some results obtained over several years of CFSD pathology research at CIAT, and provide insights on the biology of the disease coming from works on symptoms’ characterization, associated pathogens, means of transmission, carbohydrate accumulation, and management. We expect this work will contribute to a better understanding of the disease, which will reflect on lowering its impact in the Americas and minimize the risk of its spread elsewhere.

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Complete genome sequence of a novel secovirid infecting cassava in the Americas

Cited by 14 publications

References 13 publications

Proteome expansion in thePotyviridaeevolutionary radiation

Proteome expansion in thePotyviridaeevolutionary radiation

An inosine triphosphate pyrophosphatase safeguards plant nucleic acids from aberrant purine nucleotides

Cassava Frogskin Disease: Current Knowledge on a Re-Emerging Disease in the Americas

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