Complete Genome Sequence of a Variant of the Methicillin-Resistant Staphylococcus aureus ST239 Lineage, Strain BMB9393, Displaying Superior Ability To Accumulate
            <i>ica</i>
            -Independent Biofilm

Costa, Maiana O. C.; Beltrame, Cristiana Ossaille; Ferreira, Fabienne Antunes; Botelho, Ana; Lima, Nicholas Costa Barroso; Souza, Rangel Celso; Almeida, Luiz Gonzaga Paula de; Vasconcelos, Ana Tereza Ribeiro de; Nicolás, Marisa Fabiana; Figueiredo, Agnes Marie Sá

doi:10.1128/genomea.00576-13

Cited by 26 publications

(17 citation statements)

References 11 publications

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“…Kuhn et al also found no difference with adhesion genes in epidemic and sporadic MRSA clones (Kuhn et al, 2006). Gene expression studies and whole genome sequencing may assist to elucidate the reason for these differences (Costa et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Naicker

Karayem

Hoek

et al. 2016

Microbial Pathogenesis

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2 Declaration I, Preneshni Rochelle Naicker, hereby declare that the work contained in this assignment is my original work and that I have not previously submitted it, in its entirety or in part, at any university for a degree. The ability to form biofilms contributes significantly to the virulence of Staphylococcus aureus. Virulence factors may be associated with certain S. aureus lineages. However, the contribution of the genetic background of S. aureus to biofilm formation is poorly understood. This study investigated the association between the genetic background and the biofilm forming ability of clinical invasive S. aureus isolates. Secondary objectives included investigating any correlation with biofilm formation and methicillin resistance or the source of bacteraemia. Methods:The study was conducted at a 1300-bed tertiary hospital in Cape Town, South Africa. S. aureus isolates obtained from blood cultures between January 2010 and January 2012 were included. Genotypic characterization was performed by PFGE, spa typing, SCCmec typing and MLST. Thirty genotypically unique strains were assessed for phenotypic biofilm formation with the microtitre plate assay. All isolates were tested in triplicate and an average optical density, measured at a wavelength of 490nm, was determined. The biofilm forming ability of isolates with A 490 > 0.17 was considered 'weak positive' and A 490 > 0.34 'strong positive'. Isolates with A 490 ≤ 0.17 were considered non-adherent. ANOVA with Bonferroni-adjusted post-hoc tests and t-tests were used for statistical analysis of the association between biofilm forming ability and strain characteristics.Results: Fifty seven percent of isolates were capable of forming biofilms. Weak biofilm formation occurred in 40% (n=12) and strong biofilm formation in 17% (n=5) of isolates. Thirteen (43%) of the isolates were classified as non-adherent. All 5 isolates capable of strong biofilm formation belong to one spa clonal complex (spa-CC 064). Strains from spa-CC 064 were capable of higher biofilm formation than other spa clonal complexes (p=0.00002). These 5 strains belonged to MLST CC5 and CC8. Biofilm formation did not correlate with methicillin resistance and was not related to the source of bacteraemia. Conclusion:Biofilm formation correlates with the spa clonal lineage in our population of invasive S. aureus strains. High biofilm formation was associated with spa-CC 064. MLST CC5 and CC8 strains are capable of strong biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Naicker

Karayem

Hoek

et al. 2016

Microbial Pathogenesis

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“…It is generally accepted that MRSA strains develop biofilm by a PIA-independent mechanism (McCarthy et al, 2015). In particular, the BRZ lineage displays increased ability to accumulate ica -independent biofilm (Costa et al, 2013). It is worth noting that increases in the PIA concentration due to the action of SAL on MRSA strains (both CBS and BRZ) resembled that of the MSSA (Newman) strains indicating that SAL induces PIA-mediated biofilms by S. aureus independently of the methicillin susceptibility status under the conditions studied.…”

Section: Discussionmentioning

confidence: 99%

The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

et al. 2017

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Aspirin has provided clear benefits to human health. But salicylic acid (SAL) -the main aspirin biometabolite- exerts several effects on eukaryote and prokaryote cells. SAL can affect, for instance, the expression of Staphylococcus aureus virulence factors. SAL can also form complexes with iron cations and it has been shown that different iron chelating molecules diminished the formation of S. aureus biofilm. The aim of this study was to elucidate whether the iron content limitation caused by SAL can modify the S. aureus metabolism and/or metabolic regulators thus changing the expression of the main polysaccharides involved in biofilm formation. The exposure of biofilm to 2 mM SAL induced a 27% reduction in the intracellular free Fe2+ concentration compared with the controls. In addition, SAL depleted 23% of the available free Fe2+ cation in culture media. These moderate iron-limited conditions promoted an intensification of biofilms formed by strain Newman and by S. aureus clinical isolates related to the USA300 and USA100 clones. The slight decrease in iron bioavailability generated by SAL was enough to induce the increase of PIA expression in biofilms formed by methicillin-resistant as well as methicillin-sensitive S. aureus strains. S. aureus did not produce capsular polysaccharide (CP) when it was forming biofilms under any of the experimental conditions tested. Furthermore, SAL diminished aconitase activity and stimulated the lactic fermentation pathway in bacteria forming biofilms. The polysaccharide composition of S. aureus biofilms was examined and FTIR spectroscopic analysis revealed a clear impact of SAL in a codY-dependent manner. Moreover, SAL negatively affected codY transcription in mature biofilms thus relieving the CodY repression of the ica operon. Treatment of mice with SAL induced a significant increase of S aureus colonization. It is suggested that the elevated PIA expression induced by SAL might be responsible for the high nasal colonization observed in mice. SAL-induced biofilms may contribute to S. aureus infection persistence in vegetarian individuals as well as in patients that frequently consume aspirin.

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“…), and its complete genome was sequenced by Costa et al . (). All strains were stored at −70°C in 10% glycerol.…”

Section: Methodsmentioning

confidence: 97%

“…Quinones are very important compounds, as they play a key role in living cells in the respiratory chain, blood coagulation and the carboxylation of glutamates (Thomson 1997). The relationship between quinones and the biochemical processes of infecting micro-organisms led to several studies searching for synthetic bioactive compounds with antiparasitic Jorqueira et al 2006;Silva-Jr et al 2008), molluscicidal (Gallagher 1996;Santos et al 2001;Barbosa et al 2005), leishmanicidal (Teixeira et al 2001), antifungal (Gafner et al 1996), trypanocidal (Pinto et al 2000;Pinto and de Castro 2009;Salas et al 2011), and antibacterial activities (Tandon et al 2005;Jordão et al 2013;Souza et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds

et al. 2017

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Complete Genome Sequence of a Variant of the Methicillin-Resistant Staphylococcus aureus ST239 Lineage, Strain BMB9393, Displaying Superior Ability To Accumulate ica -Independent Biofilm

Cited by 26 publications

References 11 publications

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

Searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds

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