Complete genome sequence of a new botybirnavirus isolated from a phytopathogenic Alternaria alternata in China

Ma, Guoping; Liang, Zhijian; Hua, Huihui; Zhou, Tao; Wu, Xuehong

doi:10.1007/s00705-019-04189-x

Cited by 17 publications

(13 citation statements)

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“…A number of RNA viruses have been reported from Alternaria spp., including dsRNA viruses: an alternavirus [51], a partitivirus [52], botybirnaviruses [43,53,54], chrysoviruses [55], and victoriviruses (a different virus than AalVV1 from Alternaria arborescens ) [56]; positive-sense (+) single-stranded RNA (ssRNA) viruses: mitoviruses [57,58], a fusarivirus [59], and an endornavirus [60]; and other unidentified viruses [61]. This study describes the characterization of a victorivirus, AalVV1, from A. alternata , and contributes to the growing database of viruses of Alternaria spp.…”

Section: Discussionmentioning

confidence: 99%

Novel Victorivirus from a Pakistani Isolate of Alternaria alternata Lacking a Typical Translational Stop/Restart Sequence Signature

Jamal

Sato

Shahi

et al. 2019

Viruses

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The family Totiviridae currently contains five genera Totivirus, Victorivirus, Leishmavirus, Trichomonasvirus, and Giardiavirus. Members in this family generally have a set of two-open reading frame (ORF) elements in their genome with the 5′-proximal ORF (ORF1) encoding a capsid protein (CP) and the 3′-proximal one (ORF2) for RNA-dependent RNA polymerase (RdRp). How the downstream open reading frames (ORFs) are expressed is genus-specific. All victoriviruses characterized thus far appear to use the stop/restart translation mechanism, allowing for the expression of two separate protein products from bicitronic genome-sized viral mRNA, while the totiviruses use a −1 ribosomal frame-shifting that leads to a fusion product of CP and RdRp. We report the biological and molecular characterization of a novel victorivirus termed Alternaria alternata victorivirus 1 (AalVV1) isolated from Alternaria alternata in Pakistan. The phylogenetic and molecular analyses showed AalVV1 to be distinct from previously reported victoriviruses. AalVV1 appears to have a sequence signature required for the −1 frame-shifting at the ORF1/2 junction region, rather than a stop/restart key mediator. By contrast, SDS–polyacrylamide gel electrophoresis and peptide mass fingerprinting analyses of purified virion preparations suggested the expression of two protein products, not a CP-RdRp fusion product. How these proteins are expressed is discussed in this study. Possible effects of infection by this virus were tested in two fungal species: A. alternata and RNA silencing proficient and deficient strains of Cryphonectria parasitica, a model filamentous fungus. AalVV1 showed symptomless infection in all of these fungal strains, even in the RNA silencing deficient C. parasitica strain.

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Section: Discussionmentioning

confidence: 99%

Novel Victorivirus from a Pakistani Isolate of Alternaria alternata Lacking a Typical Translational Stop/Restart Sequence Signature

Jamal

Sato

Shahi

et al. 2019

Viruses

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“…Virion particles were extracted from the mycelium of A. solani strain DT-10 and stained according to the methods previously described [24,3]. Viewed under transmission electron microscope (TEM) (JEM-1230, JEOL, Tokyo, Japan), the particles of AsCV1 were isometric with a diameter of approximately 40 nm (Supplementary Fig.…”

Section: Sequence Propertiesmentioning

confidence: 99%

“…Mycoviruses identi ed in fungi and oomycetes, have genomic types of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), and DNA [1]. DsRNA mycoviruses belong to seven families including Birnaviridae, Chrysoviridae, Cystoviridae, Megabirnaviridae, Partitiviridae, Picobirnaviridae, and Quadriviridae, and a new established genus Botybirnavirus [2,3]. The family Chrysoviridae contains two genera, namely Alphachrysovirus (17 species) and Betachrysovirus (8 species).…”

Section: Introductionmentioning

confidence: 99%

“…In 1988, viral dsRNAs were detected in Alternaria alternata isolated from Japanese pear trees, which is the rst reported mycovirus in Alternaria [6]. Later, many mycoviruses had been identi ed in A. alternata, such as Alternaria alternata virus-1 (AAV-1) [7], Alternaria alternata chrysovirus 1 (AaCV1) [8], Alternaria alternata partitivirus 1 (AtPV1) [9], Alternaria alternata botybirnavirus 1 (AaBRV1) [3], and Alternaria alternata hypovirus 1 (AaHV1) [10]. In addition, several mycoviruses including Alternaria tenuissima partitivirus 1 (AttPV1) [11], Alternaria brassicicola endornavirus 1 (AbEV1) [12], Alternaria longipes dsRNA virus 1 (AlRV1) [13], Alternaria arborescens victorivirus 1 (AaVV1) [14], and Alternaria dianthicola dsRNA virus 1 (AdRV1) [15] had been reported in ve other species (A. tenuissima, A. brassicicola, A. longipes, A. arborescens, and A. dianthicola) of Alternaria.…”

Section: Introductionmentioning

confidence: 99%

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Complete genome sequence of the first chrysovirus from the phytopathogenic fungus Alternaria solani on potato in China

et al. 2021

Arch Virol

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The full genome of a double-stranded RNA (dsRNA) mycovirus, which was isolated from Alternaria solani strain DT-10 causing potato foliar disease and designated as Alternaria solani chrysovirus 1 (AsCV1), consisted of four dsRNA segments (dsRNA 1-4) with the length of 3600 bp, 3128 bp, 2996 bp, and 2714 bp, respectively. RNA-dependent RNA polymerase (RdRp, 1084 amino acids (aa)), putative capsid protein (905 aa), Alphachryso-P3 (835 aa), and Alphachryso-P4 (729 aa) were encoded by dsRNA1, dsRNA2, dsRNA3, and dsRNA4, respectively, which had the highest identities of 41.77%-72.38% to the counterparts of Helminthosporium victoriae virus 145S (HvV145S) in the genus Alphachrysovirus of the family Chrysoviridae. Moreover, the 5′-untranslated regions (UTRs) with several unique inserts (3-37 bp) and deletions (5-64 bp) of AsCV1 dsRNA 1-4 shared 51.65%-68.01% identities to those of HvV145S. Phylogenetic analysis of RdRp suggested that AsCV1 clustered the most closely with HvV145S. Based on the characteristics of distinct host, low identities of encoded proteins, special traits in 5′-UTRs of dsRNA 1-4, and phylogenetic analysis of RdRp, AsCV1 was con rmed to be a new species in the genus Alphachrysovirus. To our best knowledge, this is the rst alphachrysovirus identi ed from phytopathogenic A. solani.

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“…To our knowledge, mycoviruses from more than six families have been found to be associated with A. alternata . These include multiple dsRNA mycoviruses A. alternata partitivirus 1 (AtPV1) ( da Silva Xavier et al, 2018 ), A. alternata polymycovirus 1 (AaPmV1) ( Ma et al, 2022 ), A. alternata victorivirus 1 (AalVV1) ( Jamal et al, 2019 ), A. alternata virus 1 (AAV1) ( Wu et al, 2021 ), A. alternata chrysovirus 1 (AaCV1) ( Aoki et al, 2009 ; Okada et al, 2018 ), and A. alternata botybirnavirus 1 (AaBRV1) ( Ma et al, 2019 ), classified as the families Partitiviridae , Polymycoviridae , Totiviridae , Alternaviridae , Chrysoviridae , and unassigned, respectively. Among them, AaCV1 infection has been shown to impair host growth in phenotype, but has been shown to enhance host virulence in pathogenicity by increasing the production of AK toxins during spore germination ( Okada et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Coinfection of Two Mycoviruses Confers Hypovirulence and Reduces the Production of Mycotoxin Alternariol in Alternaria alternata f. sp. mali

Cao

et al. 2022

Front. Microbiol.

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Alternaria leaf blotch caused by Alternaria alternata apple pathotype (Alternaria mali) is an important fungal disease that affects the production of apples worldwide. Mycoviruses harbored in plant pathogenic fungi can confer hypovirulence in their hosts and have attracted widespread attention as potential biocontrol tools. In this study, the coinfection of two mycoviruses, named A. alternata chrysovirus 1 strain QY2 (AaCV1-QY2) and A. alternata magoulivirus 1 (AaMV1), respectively, were isolated from A. alternata f. sp. mali strain QY21. Sequence analyses revealed that AaCV1-QY2 virus belonged to the genus Betachrysovirus and AaMV1 virus belonged to the genus Magoulvirus. These two mycoviruses were found to be associated with hypovirulence in A. alternata, among which AaCV1-QY2 might play a relatively leading role. Because the elimination of AaMV1 from the strain QY21 does not affect the hypovirulence trait, which indicates that the virus AaCV1-QY2 can independently induce slow growth and reduce host virulence. Moreover, the presence of viruses decreased the accumulation of the mycotoxin alternariol (AOH) in A. alternata strains. Intriguingly, AaCV1-QY2/AaMV1 mycoviruses can be horizontally transmitted to other A. alternata strains, and this coinfection can promote the interspecific transmission efficiency of AaCV1-QY2. To our knowledge, this study reports the first description of the member of Chrysovirus is related to hypovirulence in Alternaria spp. that facilitates the development of biocontrol measures of A. mali Roberts.

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Complete genome sequence of a new botybirnavirus isolated from a phytopathogenic Alternaria alternata in China

Cited by 17 publications

References 18 publications

Novel Victorivirus from a Pakistani Isolate of Alternaria alternata Lacking a Typical Translational Stop/Restart Sequence Signature

Novel Victorivirus from a Pakistani Isolate of Alternaria alternata Lacking a Typical Translational Stop/Restart Sequence Signature

Complete genome sequence of the first chrysovirus from the phytopathogenic fungus Alternaria solani on potato in China

Coinfection of Two Mycoviruses Confers Hypovirulence and Reduces the Production of Mycotoxin Alternariol in Alternaria alternata f. sp. mali

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