Complete genome sequence of a tentative novel capillovirus isolated from Gerbera jamesonii

Abstract

The currently named gerbera virus A (GeVA) has been identified as a novel capillovirus with a complete genome of 6929 nucleotides (nt) (GenBank accession no. OM525829.1). GeVA was detected in Gerbera jamesonii using high-throughput RNA sequencing analysis. The complete genome represents a single linear RNA with two open reading frames (ORF). The large ORF encodes a polyprotein contains four domains, while the smaller ORF encodes a movement protein. The addition of find two UUAGGU promoters for subgenomic RNA (sgRNA) transcription was also identified in this study. BLAST analysis demonstrated that it shared the highest identity with the rubber tree capillovirus 1 (MN047299.1) (complete nucleotide: 68.54%; polyprotein amino acid: 44.53%). Phylogenetic analysis of complete genome and replication protein sequences place GeVA alongside other genus Capillovirus in the family Betaflexiviridae. These data suggest that GeVA is a new member of the genus Capillovirus.