Complete Genome Sequence of a Common Midwife Toad Virus-Like Ranavirus Associated with Mass Mortalities in Wild Amphibians in the Netherlands

Beurden, Steven J. van; Hughes, Joseph; Saucedo, Bernardo; Rijks, Jolianne M.; Kik, Marja; Haenen, O.L.M.; Engelsma, Marc; Gröne, Andrea; Verheije, Monique H.; Wilkie, Gavin S.

doi:10.1128/genomea.01293-14

Cited by 18 publications

(20 citation statements)

References 8 publications

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“…The Devon-CMTV-GB2 isolate shared 100% sequence identity at the MCP with an isolate from a captive Hermann’s tortoise ( Testudo hermanii ) from a zoo in Switzerland (accession numbers AF114154 and KP266741 [ 17 , 21 ]) but shared highest sequence identity across all six loci with pike-perch iridovirus (99.46%; Fig 3B ; S4 Table ) isolated from apparently healthy pike-perch ( Sander lucioperca ) fingerlings in Finland in 1995 [ 44 , 45 ]. The Surrey-CMTV-GB1 isolate was 100% identical across the partial MCP sequence to Rana esculenta virus from Italy (accession FJ358611 [ 46 ]), but among ranaviruses with whole genomes enabling comparison across all six loci, Surrey-CMTV-GB1 showed highest sequence identity to CMTV isolated in the Netherlands in 2013 (99.1%; Fig 3B ; S4 Table ) [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Screening of a long-term sample set reveals two Ranavirus lineages in British herpetofauna

et al. 2017

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Reports of severe disease outbreaks in amphibian communities in mainland Europe due to strains of the common midwife toad virus (CMTV)-like clade of Ranavirus are increasing and have created concern due to their considerable population impacts. In Great Britain, viruses in another clade of Ranavirus–frog virus 3 (FV3)-like—have caused marked declines of common frog (Rana temporaria) populations following likely recent virus introductions. The British public has been reporting mortality incidents to a citizen science project since 1992, with carcasses submitted for post-mortem examination, resulting in a long-term tissue archive spanning 25 years. We screened this archive for ranavirus (458 individuals from 228 incidents) using molecular methods and undertook preliminary genotyping of the ranaviruses detected. In total, ranavirus was detected in 90 individuals from 41 incidents focused in the north and south of England. The majority of detections involved common frogs (90%) but also another anuran, a caudate and a reptile. Most incidents were associated with FV3-like viruses but two, separated by 300 km and 16 years, involved CMTV-like viruses. These British CMTV-like viruses were more closely related to ranaviruses from mainland Europe than to each other and were estimated to have diverged at least 458 years ago. This evidence of a CMTV-like virus in Great Britain in 1995 represents the earliest confirmed case of a CMTV associated with amphibians and raises important questions about the history of ranavirus in Great Britain and the epidemiology of CMTV-like viruses. Despite biases present in the opportunistic sample used, this study also demonstrates the role of citizen science projects in generating resources for research and the value of maintaining long-term wildlife tissue archives.

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Section: Resultsmentioning

confidence: 99%

Screening of a long-term sample set reveals two Ranavirus lineages in British herpetofauna

et al. 2017

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“…Conventional PCR and sequencing of the major capsid protein from liver samples of both animals revealed FV3, a clade of ranaviruses that, unlike CMTV, has not been reported to occur in Dutch nature ( 3 , 13 , 14 ). Subsequently, a 10% organ suspension from one animal was mixed with 1% antibiotics and inoculated on epithelioma papulosum cyprini cells.…”

Section: Genome Announcementmentioning

confidence: 94%

“…Subsequently, a 10% organ suspension from one animal was mixed with 1% antibiotics and inoculated on epithelioma papulosum cyprini cells. Once full cytopathic effect was observed, the virus was purified by high-speed ultracentrifugation on a 36% sucrose cushion as described previously ( 14 ). After resuspension in ice-cold phosphate-buffered saline (PBS), the DNA was extracted with the QIAamp DNA blood minikit (Qiagen) according to the manufacturer’s protocol.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Frog virus 3 , Isolated from a Strawberry Poison Frog ( Oophaga pumilio ) Imported from Nicaragua into the Netherlands

et al. 2017

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“…Many more outbreaks of ranavirosis have now been documented across continental Europe. Perhaps the largest scale morbidity and mortality event involving a CMTV-like virus occurred in 2010 in the Netherlands (Kik et al, 2011;van Beurden et al, 2014). The event involved more than 1000 individuals, mostly Pelophylax spp.…”

Section: Ranavirusesmentioning

confidence: 99%

Emerging infectious disease threats to European herpetofauna

Allain¹,

Duffus²

2019

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In the past decade, infectious disease threats to European herpetofauna have become better understood. Since the 1990s, three major emerging infections in amphibians have been identified (Batrachochytrium dendrobatidis, B. salamandrivorans, and ranaviruses) as well as at least one of unknown status (herpesviruses), while two major emerging infections of reptiles (Ophidiomyces ophiodiicola and ranaviruses) have been identified in wild European populations. The effects of emerging infections on populations have ranged from non-existent to local extirpation. In this article, we review these major infectious disease threats to European herpetofauna, including descriptions of key mortality and/or morbidity events in Europe of their emergence, and address both the distribution and the host diversity of the agent. Additionally, we direct the reader to newly developed resources that facilitate the study of infectious agents in herpetofauna and again stress the importance of an interdisciplinary approach to examining these infectious diseases.

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Complete Genome Sequence of a Common Midwife Toad Virus-Like Ranavirus Associated with Mass Mortalities in Wild Amphibians in the Netherlands

Cited by 18 publications

References 8 publications

Screening of a long-term sample set reveals two Ranavirus lineages in British herpetofauna

Screening of a long-term sample set reveals two Ranavirus lineages in British herpetofauna

Complete Genome Sequence of Frog virus 3 , Isolated from a Strawberry Poison Frog ( Oophaga pumilio ) Imported from Nicaragua into the Netherlands

Emerging infectious disease threats to European herpetofauna

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