Complete Genome Sequence of Acinetobacter radioresistens Strain LH6, a Multidrug-Resistant Bacteriophage-Propagating Strain

Crippen, Clay S.; Huynh, Steven; Miller, William G.; Parker, Craig T.; Szymanski, Christine M.

doi:10.1128/mra.00929-18

Cited by 7 publications

(11 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We previously sequenced strain A. radioresistens LH6 described in this study. LH6 lacks plasmids, but encodes several putative MBLs, which can account for its resistance profile ( Crippen et al, 2018 ). Interestingly, LH6 also encodes bla OXA–2 3 with 100% amino acid homology to the A. baumannii ASM74664v1 homolog, but no broad carbapenem, cephalosporin or β-lactamase resistance phenotype was observed ( Table 1 and Supplementary Figure S4 ), which is normally associated with the presence of this gene ( Pfeifer et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, 5/7 of the isolated phages were segmented RNA phages that are known to undergo recombination at high rates ( Simon-Loriere and Holmes, 2011 ). In addition, genomic sequencing of A. radioresistens LH6 ( Crippen et al, 2018 ) identified two possible chromosomal prophages, including one Mu-like, that may also be involved in AMR gene transduction ( Braid et al, 2004 ). One of these prophages was indeed isolated after induction with mitomycin C and is being described in a separate manuscript (Crippen et al, in preparation).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Multidrug Resistant Acinetobacter Isolates Release Resistance Determinants Through Contact-Dependent Killing and Bacteriophage Lysis

et al. 2020

Self Cite

View full text Add to dashboard Cite

Antimicrobial resistance is an ancient bacterial defense mechanism that has rapidly spread due to the frequent use of antibiotics for disease treatment and livestock growth promotion. We are becoming increasingly aware that pathogens, such as members of the genus Acinetobacter, are precipitously evolving drug resistances through multiple mechanisms, including the acquisition of antibiotic resistance genes. In this study, we isolated three multidrug resistant Acinetobacter species from birds on a free-range farm. Acinetobacter radioresistens, Acinetobacter lwoffii, and Acinetobacter johnsonii were isolated from hens, turkeys and ducks and were resistant to 14 clinically relevant antibiotics, including several listed by the World Health Organization as essential medicines. Co-culturing any of the three Acinetobacter species with Acinetobacter baumannii resulted in contact-dependent release of intact resistance determinants. We also isolated several lytic bacteriophages and selected two of these phages to be included in this study based on differences in plaquing characteristics, nucleic acid content and viral morphology. Both phages released host DNA, including antibiotic resistance genes during cell lysis and we demonstrated that these resistance determinants were transferable to a naïve strain of Escherichia coli. This study demonstrates that contact-dependent competition between bacterial species can readily contribute to DNA release into the environment, including antibiotic resistance determinants. We also highlight that the constant lysis and turnover of bacterial populations during the natural lifecycle of a lytic bacteriophage is an underappreciated mechanism for the liberation of DNA and subsequent genetic exchange.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Multidrug Resistant Acinetobacter Isolates Release Resistance Determinants Through Contact-Dependent Killing and Bacteriophage Lysis

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the identification of the induced prophage SLAP1, primers were designed that target the capsid genes of prophage 1 and prophage 2 [ 6 ]. Prophage 1 was detected with the primers pP1capsid-F and pP1capsid-R, prophage 2 was detected with the primers pP2capsid-F and pP2capsid-R (primers listed in Table S1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Thus, the majority of phage screens have been directed toward this pathogen, and the host range for these Acinetobacter -infecting phages will be limited to A. baumannii . Recently, we isolated the Acinetobacter radioresistens strain LH6 from chicken feces on a free-range farm [ 6 ]. We found that this multidrug resistant reservoir strain encodes genes associated with resistance to toxic metals and quaternary ammonium compounds [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, the LOS is produced via the Raetz pathway, wherein Kdo 2 -lipid A is built in the inner leaflet of the inner membrane and the oligosaccharide core is assembled sequentially onto the first Kdo sugar moiety, yielding LOS. The LOS is then flipped into the periplasmic space and transferred to the outer membrane via the Lpt pathway [ 15 ], readily recognizable from the genomic sequence of LH6 [ 6 ]. Another sugar structure that is commonly produced by acinetobacters is the PNAG polymer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

RNA and Sugars, Unique Properties of Bacteriophages Infecting Multidrug Resistant Acinetobacter radioresistens Strain LH6

Crippen

Zhou

Andresen

et al. 2021

Viruses

Self Cite

View full text Add to dashboard Cite

Bacteriophages (phages) are predicted to be the most ubiquitous biological entity on earth, and yet, there are still vast knowledge gaps in our understanding of phage diversity and phage–host interactions. Approximately one hundred Acinetobacter-infecting DNA viruses have been identified, and in this report, we describe eight more. We isolated two typical dsDNA lytic podoviruses (CAP1–2), five unique dsRNA lytic cystoviruses (CAP3–7), and one dsDNA lysogenic siphovirus (SLAP1), all capable of infecting the multidrug resistant isolate Acinetobacter radioresistens LH6. Using transmission electron microscopy, bacterial mutagenesis, phage infectivity assays, carbohydrate staining, mass-spectrometry, genomic sequencing, and comparative studies, we further characterized these phages. Mutation of the LH6 initiating glycosyltransferase homolog, PglC, necessary for both O-linked glycoprotein and capsular polysaccharide (CPS) biosynthesis, prevented infection by the lytic podovirus CAP1, while mutation of the pilin protein, PilA, prevented infection by CAP3, representing the lytic cystoviruses. Genome sequencing of the three dsRNA segments of the isolated cystoviruses revealed low levels of homology, but conserved synteny with the only other reported cystoviruses that infect Pseudomonas species. In Pseudomonas, the cystoviruses are known to be enveloped phages surrounding their capsids with the inner membrane from the infected host. To characterize any membrane-associated glycoconjugates in the CAP3 cystovirus, carbohydrate staining was used to identify a low molecular weight lipid-linked glycoconjugate subsequently identified by mutagenesis and mass-spectrometry as bacterial lipooligosaccharide. Together, this study demonstrates the isolation of new Acinetobacter-infecting phages and the determination of their cell receptors. Further, we describe the genomes of a new genus of Cystoviruses and perform an initial characterization of membrane-associated glycoconjugates.

show abstract

Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing

Deguenon

Travanty

Zhu

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiology of human and animal diseases is an active area of research, little is known about the microorganisms associated with these insects. We examined the diversity of wild-caught black blow fly endogenous (internal body) and exogenous (external body) microbial communities using 16S rRNA gene sequencing. Overall, 27 phyla, 171 families and 533 genera were detected, and diversity was significantly higher (P < 0.05) on external body surfaces. At the genus level, Dysgonomonas, Ignatzschineria, Acinetobacter, Vagococcus, Myroides, and Wohlfahrtiimonas were predominant. Cloning and sequencing of nearly full-length fragments of the 16S rRNA gene showed that some of the species identified are known to be pathogenic to humans, animals, and plants. Myroides odoratimimus and Acinetobacter radioresistens are well-known, multi-drug resistant bacteria. These results provide a snapshot of the microbial communities harbored by adult black blow flies and call for more comprehensive studies to better characterize the role these flies may play in the transmission of pathogenic microorganisms.

show abstract

Complete Genome Sequence of Acinetobacter radioresistens Strain LH6, a Multidrug-Resistant Bacteriophage-Propagating Strain

Abstract: Antimicrobial resistance is a major problem worldwide. Understanding the interplay between drug-resistant pathogens, such as Acinetobacter baumannii and related species, potentially acting as environmental reservoirs is critical for preventing the spread of resistance determinants.

Cited by 7 publications

References 9 publications

Multidrug Resistant Acinetobacter Isolates Release Resistance Determinants Through Contact-Dependent Killing and Bacteriophage Lysis

Multidrug Resistant Acinetobacter Isolates Release Resistance Determinants Through Contact-Dependent Killing and Bacteriophage Lysis

RNA and Sugars, Unique Properties of Bacteriophages Infecting Multidrug Resistant Acinetobacter radioresistens Strain LH6

Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing

Contact Info

Product

Resources

About