Complete Genome Sequence of Aggregatibacter actinomycetemcomitans Strain IDH781

Abstract

We report here the complete genomic sequence and methylome of Aggregatibacter actinomycetemcomitans strain IDH781. This rough strain is used extensively as a model organism to characterize localized aggressive periodontitis pathogenesis, the basic biology and oral cavity colonization of A. actinomycetemcomitans, and its interactions with other members of the oral microbiome.

Genetic and Biochemical Characterization of Halogenation and Drug Transportation Genes Encoded in the Albofungin Biosynthetic Gene Cluster

“…The gDNA sample was subjected to PacBio RSII circular consensus sequencing. After sequencing, the results were assembled using the PacBio Hierarchical Genome Assembly Process version 2 (HGAP_Assembly.2) ( 70 ). The resulting 30 contigs were annotated using antiSMASH to search for cryptic biosynthetic gene clusters.…”

Whole genome sequencing of Streptomyces actuosus ISP-5337, Streptomyces sioyaensis B-5408, and Actinospica acidiphila B-2296 reveals secondary metabolomes with antibiotic potential

“…Each genome was sequenced on the PacBio RS II with one SMRTcell per genome. After sequencing, each genome was assembled using the PacBio Hierarchical Genome Assembly Process version 2 (HGAP_Assembly.2) [ 39 ], contigs with poor coverage or QV scores were removed with a custom Python script, contigs were merged using Circlator (v 1.0.2), and polished using PacBio’s resequencing pipeline. The resulting assemblies were annotated using Prokka v1.11 [ 40 ] with a custom genus database [ 41 ].…”