Complete Genome Sequence of an Extremely Halophilic Archaeon from Great Salt Lake, Halobacterium sp. GSL-19

Abstract

An extremely halophilic archaeon, Halobacterium sp. strain GSL-19, was isolated from the north arm of Great Salt Lake in Utah. Single-molecule real-time (SMRT) sequencing was used to establish a GC-rich 2.3-Mbp genome composed of a circular chromosome and 2 plasmids, with 2,367 predicted genes, including 1 coding a CTAG-methylase widely distributed among Haloarchaea .

Genomic Analysis of Haloarchaea from Diverse Environments, including Permian Halite, Reveals Diversity of Ultraviolet Radiation Survival and DNA Photolyase Gene Variants

“…The remaining strains were recent isolates ( Figure 1 ): Halobacterium sp. GSL-19 from the North Arm of the Great Salt Lake, UT, USA [ 35 ], Salarchaeum sp. JOR-1 from the east coast of the Dead Sea, Jordan [ 36 ], Halorubrum sp.…”

“…NRC-1 genome (the first Haloarchaeon to be fully sequenced) revealed the presence of genes for a wide variety of DNA repair systems, including direct photorepair, nucleotide excision repair, mismatch repair, oxidative repair, non-homologous end-joining, and recombinational repair [ 10 , 27 , 28 ]. The genome was found to contain two phr genes coding for photolyase-like proteins and the uvr ABC and D genes that code for DNA excinuclease activity [ 27 , 28 ]. All of these gene transcripts and proteins were identified by transcriptomic and proteome analyses, respectively [ 13 , 29 ].…”

Genome Sequence of Halobacterium sp. Strain BOL4-2, Isolated and Cultured from Salar de Uyuni, Bolivia

“…An extreme halophile, strain BOL4-2, was isolated from Salar de Uyuni, Department of Potosí, Bolivia, the world's largest salt flat and an environment remarkable for its high elevation, cold temperatures, and UV radiation exposure ( 1 – 4 ). Such environments are of significant interest to the astrobiology community due to their multiple extremes ( 4 – 11 ).…”