Complete genome sequence of Arthrobacter sp. ZXY-2 associated with effective atrazine degradation and salt adaptation

Zhao, Xinyue; Ma, Fang; Chen, Feng; Bai, Shunwen; Yang, Jixian; Wang, Li

doi:10.1016/j.jbiotec.2017.03.010

Cited by 46 publications

(18 citation statements)

References 20 publications

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“…Arthrobacter sp. strain ZXY-2 ( Actinobacteria : Micrococcaceae ) was previously isolated from the Jilin Pesticide Plant, China [ 17 ]. Strain ZXY-2 was cultured in an enrichment medium that consisted of 6 g L −1 Na 2 HPO 4 ·12 H 2 O, 50 mg L −1 atrazine, and 2.212 g L −1 sucrose.…”

Section: Methodsmentioning

confidence: 99%

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Characterisation of an efficient atrazine-degrading bacterium, Arthrobacter sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications

Zhao

Wang

et al. 2018

Biotechnol Biofuels

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BackgroundThe isolation of atrazine-degrading microorganisms with specific characteristics is fundamental for bioaugmenting the treatment of wastewater containing atrazine. However, studies describing the specific features of such microorganisms are limited, and further investigation is needed to improve our understanding of bioaugmentation.Results and conclusionsIn this study, strain Arthrobacter sp. ZXY-2, which displayed a strong capacity to degrade atrazine, was isolated and shown to be a potential candidate for bioaugmentation. The factors associated with the biodegrading capacity of strain ZXY-2 were investigated, and how these factors likely govern the metabolic characteristics that control bioaugmentation functionality was determined. The growth pattern of Arthrobacter sp. ZXY-2 followed the Haldane–Andrews model with an inhibition constant (Ki) of 52.76 mg L−1, indicating the possible augmentation of wastewater treatment with relatively high atrazine concentrations (> 50 ppm). Real-time quantitative PCR (RT-qPCR) results showed a positive correlation between the atrazine degradation rate and the expression levels of three functional genes (trzN, atzB, and atzC), which helped elucidate the role of strain ZXY-2 in bioaugmentation. In addition, multiple copies of the atzB gene were putatively identified, explaining the higher expression levels of this gene than those of the other functional genes. Multiple copies of the atzB gene may represent a compensatory mechanism that ensures the biodegradation of atrazine, a feature that should be exploited in future bioaugmentation applications.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1113-0) contains supplementary material, which is available to authorised users.

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Section: Methodsmentioning

confidence: 99%

“…Notably, Arthrobacter sp. strain ZXY-2 was reported to have a high atrazine degradation rate [ 17 ]. In this study, strain ZXY-2 was selected to investigate the factors that are important in determining a high atrazine degradation rate.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of an efficient atrazine-degrading bacterium, Arthrobacter sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications

Zhao

Wang

et al. 2018

Biotechnol Biofuels

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“…NEA-D and Ensifer sp. CX-T [24,33]. In addition to the above three genomic combinations, trzN-atzBCDEF was found in Micrococcus luteus sp.…”

Section: Discussionmentioning

confidence: 99%

“…[34]. In the Arthrobacter strains, the degrading genes were mainly a combination of trz-atzBC genes, for example the 160-kb plasmid of Arthrobacter auresces TC1 contained trzN, atzB and atzC genes [22,24], and in Arthrobacter globiformis the trzN and atzC genes were found [13]. DNA sequencing results showed that the nucleotide sequences of the three degrading genes (trzN and atzB and C from FM326) were more than 99% similar to those of Arthrobacter sp.…”

Section: Discussionmentioning

confidence: 99%

“…Another atrazine degradation pathway is found in Arthrobacter sp. with the genes atzA, B, and C [23], or trzN-atzB, and C [21,24], which encode the degrading enzymes AtzA, B, and C, or TrzN-AtzB, and C, respectively. Although atrazine can be degraded into cyanuric acid without further degradation, the gene atzD was amplified on the chromosome of Arthrobacter sp.…”

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Atrazine Degradation Pathway and Genes of <i>Arthrobacter</i> sp. FM326

Li¹,

Zhan²,

Chen³

et al. 2020

Pol. J. Environ. Stud.

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Atrazine is a triazine herbicide selectively used on maize, sorghum, cotton and other crops [1]. It is stable and persistent in soil, which has brought huge pollution to soil, surface water and groundwater [2]. In addition, atrazine is a hormone disruptor, harmful to human health and the sustainable development of the ecosystem [3-4]. At present, the way to deal with pesticide pollution is to control the quantity of pesticides in the environment, including landfill treatment of polluted soil (which is also expensive). Microbial remediation is a good alternative method that has high efficiency and leaves no residue [5-7]. Atrazine can be deaminated, dechlorinated, and ring cracked by microorganisms. To date, many atrazinedegrading microbes have been found [8-18], and some highly efficient atrazine-degrading strains have been constructed by genetic engineering [19]. Among those, the degradation pathways of the Pseudomonas sp. ADP and Arthrobacter aurescens TC1 strains have been most extensively studied, and they are used as the model strains for the study of atrazine biodegradation [20-22]. The degradation genes atzA, B, C, D, E, and F,

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Green synthesis of silver‐doped metal hexacyanoferrates nanostructures for efficient cleanup of endocrine‐disrupting pesticides

Rani,

Choudhary,

Shanker

2023

Env Prog and Sustain Energy

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Herein, inexpensive and eco‐friendly approach for the green synthesis of silver‐doped metal hexacyanoferrates (Ag@MHCFs) nanocomposite using green tea extract has been reported. Silver‐doped MHCF nanocatalysts were used to photo‐degradation endocrine disruptor pesticides, namely endosulfan (ES) and atrazine (AT), from water under direct Sunlight. Spectroscopic and electron microscopic techniques confirmed the successful synthesis of nanomaterials. Ag@FeHCF observed maximum degradation efficiency due to their high surface area (89.3 m2g−1), significant zeta potential (−43.4 mV), lower band gap (1.5 eV), and low photoluminescence intensity as compared to other Ag doped MHCFs. Best degradation results showed with concentration amount (5 mg L−1), dose (20 mg of ES; 15 mg of AT), at neutral pH under sunlight irradiation. Degradation up to 98% for ES and 96% for AT was reported. The degradation ensued by Langmuir adsorption and first‐order kinetics. GC–MS analysis showed the degradation of pesticides into CO2, H2O, and harmless minor metabolites under Sunlight. Ag@FeHCF have indicated high reusability (n = 10), ensuring their charge separation, stability, and sustainability. Ag@MHCF nanoparticles may show as substitute catalysts for industrial use with a fervent scope.

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Complete genome sequence of Arthrobacter sp. ZXY-2 associated with effective atrazine degradation and salt adaptation

Cited by 46 publications

References 20 publications

Characterisation of an efficient atrazine-degrading bacterium, Arthrobacter sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications

Characterisation of an efficient atrazine-degrading bacterium, Arthrobacter sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications

Atrazine Degradation Pathway and Genes of <i>Arthrobacter</i> sp. FM326

Green synthesis of silver‐doped metal hexacyanoferrates nanostructures for efficient cleanup of endocrine‐disrupting pesticides

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