Complete genome sequence of Bacillus licheniformis BL-010

Sheng, He; Feng, Kun; Ding, Tao; Huang, Kan; Yan, Hai; Liu, Xiaolu; Zhang, Zhongbao

doi:10.1016/j.micpath.2018.03.037

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…We applied a bioinformatics approach, in which BLASTp was performed using the protein coding sequence of bacteria laccases and chitinases as a query against the BL010 genome database. Whole genome information for BL010 had been previously obtained in our laboratory [25]. To determine the evolutionary relationship between the two BL010 bacterial enzymes and other laccases and chitinases, we performed a phylogenetic analysis using the amino acid sequences of BL010 laccase, 27 other laccases, chitinase, and 25 other chitinases obtained from the National Center for Biotechnology Information (NCBI) database using neighbor joining methods with 1000 bootstrap replications performed by Molecular Evolutionary Genetics Analysis (MEGA) software v.6.06 [46].…”

Section: Methodsmentioning

confidence: 99%

“…An aflatoxin oxidase from A. tabescens [20], manganese peroxidase from the white rot fungus Phanerochaete sordida YK-624 [21], as well as laccases and chitinases [22,23,24] have been shown to have an AFB1 degrading ability. In addition, whole genome information for BL010 was previously obtained in our laboratory [25] and checked for the presence of any sequences encoding these two enzymes that could be responsible for the previously revealed activity.…”

Section: Introductionmentioning

confidence: 99%

“…There are abundant, diverse microorganism resources in the soil, and recent studies have applied many of these to biodegrade stubborn compounds [26,27]. In a previous study, we isolated and screened a new strain, Bacillus licheniformis (BL010), from humid and loose surface soil samples of corn fodder stacks [25]. In the current study, we investigate and explore the degradation and detoxification effects of BL010 on AFB1 liquid cultures and cell-free extract in both induced and uninduced form.…”

Section: Introductionmentioning

confidence: 99%

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Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain

Wang

Zhang

Yan

et al. 2018

Toxins

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Aflatoxin B1 (AFB1), a pollutant of agricultural products, has attracted considerable attention in recent years, due to its potential impact on health. In the present study, Bacillus licheniformis (BL010) was demonstrated to efficiently degrade AFB1, reducing over 89.1% of the toxin content within 120 h. A crude enzyme solution of BL010 exhibited the highest degradation level (97.3%) after three induction periods. However, uninduced BL010 bacteria was not capable of reducing AFB1. Furthermore, high performance liquid chromatography (HPLC) analysis showed that while a cell-free extract caused a significant decrease in AFB1 content (93.6%, p < 0.05), cell culture fluid treatment did not significantly degrade AFB1. The biotransformation products of AFB1 were detected and further identified by quadrupole time-of-flight liquid chromatography–mass spectrometry (LC-Q-TOF/MS); these corresponded to a molecular formula of C12H14O4. A sequence analysis of whole BL010 genes with a bioinformatics approach identified the secondary structures of two degrading enzymes (Chia010 and Lac010), providing an important basis for subsequent homology modeling and functional predictions.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain

Wang

Zhang

Yan

et al. 2018

Toxins

Self Cite

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“…Actually, it has become a current research hotspot to discover genetic resources for commercial needs by analyzing the complete genome sequence of strains. He et al [52] analyzed the complete genome sequence of B. licheniformis BL-010 capable of degrading aflatoxin B 1 , and studied the genes related to aflatoxin B 1 degradation in depth at the gene level, which is important and valuable for improving the yield of aflatoxin B 1 detoxifying enzymes. Mpofu et al [53] determined the complete genome sequence of B. licheniformis TAB7, which can be used as a compost deodorizing strain with plant growth-promoting potential.…”

Section: Discussionmentioning

confidence: 99%

Complete genome sequence of Bacillus Licheniformis NWMCC0046, a candidate for the laundry industry

Zhou

Zeng

et al. 2022

J Basic Microbiol

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In this study, selected properties of protease and the complete genome sequence of Bacillus licheniformis NWMCC0046 were investigated, to discover laundry applications and other potential probiotic properties of this strain. Partial characterization of B. licheniformis NWMCC0046 showed that its protease has good activity both in alkaline environments and at low temperatures. Also, the protease is compatible with commercial detergents and can be used as a detergent additive for effective stain removal at low temperatures. The complete genome sequence of B. licheniformis NWMCC0046 is comprised of a 4,321,565 bp linear chromosome with a G + C content of 46.78% and no plasmids. It had 4504 protein‐encoding genes, 81 transfer RNA (tRNA) genes, and 24 ribosomal RNA (rRNA) genes. Genomic analysis revealed genes involved in exocellular enzyme production and probiotic properties. In addition, genomic sequence analysis revealed specific genes encoding carbohydrate metabolism pathways, resistance, and cold adaptation capacity. Overall, protease properties show its potential as a detergent additive enzyme. The complete genome sequence information of B. licheniformis NWMCC0046 was obtained, and functional prediction revealed its numerous probiotic properties.

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“…Genome-wide analysis plays an important role in discovering gene functions and better understanding the biological functions, pathogenic mechanisms, toxin degradation mechanisms, and beneficial mechanisms of bacteria. For example, genome-wide analysis can reveal the active enzyme of Bacillus licheniformis BL-0101 that acts on AFB 1 [ 12 ]. In addition, the mechanisms by which food-grade bacteria function in the gut were revealed by postgenomics, comparative genomics, and other analyses [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

A Bacillus subtilis Strain ZJ20 with AFB1 Detoxification Ability: A Comprehensive Analysis

Huang,

Guo,

Jia

et al. 2023

Biology

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As a class I carcinogen, aflatoxin can cause serious damage to various tissues and organs through oxidative stress injuries. The liver, as the target organ of AFB1, is the most seriously damaged. Biological methods are commonly used to degrade AFB1. In our study, the aflatoxin B1-degrading strain ZJ20 was screened from AFB1-contaminated feed and soil, and the degradation of AFB1 by ZJ20 was investigated. The whole genome of strain ZJ20 was analyzed, revealing the genomic complexity of strain ZJ20. The 16S rRNA analysis of strain ZJ20 showed 100% identity to Bacillus subtilis IAM 12118. Through whole gene functional annotation, it was determined that ZJ20 has high antioxidant activity and enzymatic activity; more than 100 CAZymes and 11 gene clusters are involved in the production of secondary metabolites with antimicrobial properties. In addition, B. subtilis ZJ20 was predicted to contain a cluster of genes encoding AFB1-degrading enzymes, including chitinase, laccase, lactonase, and manganese oxidase. The comprehensive analysis of B. subtilis provides a theoretical basis for the subsequent development of the biological functions of ZJ20 and the combinatorial enzyme degradation of AFB1.

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Complete genome sequence of Bacillus licheniformis BL-010

Cited by 11 publications

References 12 publications

Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain

Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain

Complete genome sequence of Bacillus Licheniformis NWMCC0046, a candidate for the laundry industry

A Bacillus subtilis Strain ZJ20 with AFB1 Detoxification Ability: A Comprehensive Analysis

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