Complete Genome Sequence of Bacillus velezensis Strain S4, Isolated from Biochar-Treated Soil

Hempel, Priscilla P.; Yao, Mengyin; Yannarell, Sarah M.; Шевченко, О. Г.; Vogt, Franziska; Donofrio, Nicole; Maresca, Julia A.

doi:10.1128/mra.00352-20

Cited by 3 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To assess the taxonomic identity of BV379, multilocus sequence typing included BV379 and 32 additional Bacillus strains. BV379 closely aligns with B. velezensis genomes (Figure 1), including B. velezensis strain S4, which was isolated from biochar-treated soil [102]. The whole genome alignments performed using average nucleotide identity (ANI) analysis confirmed the phylogenetic findings (Table S1).…”

Section: Taxonomic Classificationsupporting

confidence: 60%

Section: Taxonomic Classificationmentioning

confidence: 62%

“…BV379 closely aligns with B . velezensis genomes ( Figure 1 ), including B. velezensis strain S4, which was isolated from biochar-treated soil [ 102 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Genetic and Phenotypic Characterization of Bacillus velezensis Strain BV379 for Human Probiotic Applications

Brutscher,

Gebrechristos,

Garvey

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Bacterial spore-forming Bacillaceae species, including Bacillus subtilis and Heyndrickxia coagulans, are increasingly utilized for probiotic dietary supplementation. Bacillus velezensis is a Bacillus species that is frequently used as a direct-fed microbial in animal feed but less so as a probiotic for humans. The objective of this study was to characterize the suitability of the Bacillus velezensis strain BV379 for probiotic applications by (1) in silico screening for both adverse genetic elements and putatively beneficial traits, (2) in vitro evaluation of interactions with human intestinal epithelial cells, and (3) in vitro characterization of BV379 spore viability at various temperatures, pH, and in the presence of bile salt. In silico screening of the BV379 genome revealed few genes encoding Bacillaceae-associated toxins, virulence factors, and enzymes involved in the production of toxins. While BV379 encodes five antimicrobial resistance genes, minimum inhibitory concentration assays determined that BV379 is susceptible to all eight clinically relevant antibiotics tested. Preliminary cell culture experiments showed that BV379 lysates did not adversely impact human intestinal epithelial cell viability and monolayer permeability. It was also determined that BV379 spores can easily tolerate the harsh pH, bile salt, and microaerobic conditions typical of the GI tract. Altogether, the results presented herein support the safety and potential of Bacillus velezensis strain BV379 for use as an oral probiotic.

show abstract

Section: Taxonomic Classificationsupporting

confidence: 60%

Section: Taxonomic Classificationmentioning

confidence: 62%

See 1 more Smart Citation

Genetic and Phenotypic Characterization of Bacillus velezensis Strain BV379 for Human Probiotic Applications

Brutscher,

Gebrechristos,

Garvey

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

“…In the sesame seed brands, Bacillus was the only taxon identified which contains human foodborne pathogens. Although this genus contains species which can cause foodborne illness, Bacillus is also a ubiquitous environmental pathogen and has been isolated from soil [ 85 ] and a variety of food products [ 32 , 33 ].…”

Section: Resultsmentioning

confidence: 99%

Microbiomes of commercially-available pine nuts and sesame seeds

et al. 2021

View full text Add to dashboard Cite

Metagenomic analysis of food is becoming more routine and can provide important information pertaining to the shelf life potential and the safety of these products. However, less information is available on the microbiomes associated with low water activity foods. Pine nuts and sesame seeds, and food products which contain these ingredients, have been associated with recalls due to contamination with bacterial foodborne pathogens. The objective of this study was to identify the microbial community of pine nuts and sesame seeds using targeted 16S rRNA sequencing technology. Ten different brands of each seed type were assessed, and core microbiomes were determined. A total of 21 and 16 unique taxa with proportional abundances >1% in at least one brand were identified in the pine nuts and sesame seeds, respectively. Members of the core pine nut microbiome included the genera Alishewanella, Aminivibrio, Mycoplasma, Streptococcus, and unassigned OTUs in the families of Desulfobacteraceae and Xanthomonadaceae. For sesame seeds, the core microbiome included Aminivibrio, Chryseolina, Okibacterium, and unassigned OTUs in the family Flavobacteriaceae. The microbiomes of these seeds revealed that these products are dominated by environmental bacterial genera commonly isolated from soil, water, and plants; bacterial genera containing species known as commensal organisms were also identified. Understanding these microbiomes can aid in the risk assessment of these products by identifying food spoilage potential and community members which may co-enrich with foodborne bacterial pathogens.

show abstract

A Bacillus velezensis strain shows antimicrobial activity against soilborne and foliar fungi and oomycetes

Wockenfuss,

Chan,

Cooper

et al. 2024

Front. Fungal Biol.

Self Cite

View full text Add to dashboard Cite

Biological control uses naturally occurring antagonists such as bacteria or fungi for environmentally friendly control of plant pathogens. Bacillus spp. have been used for biocontrol of numerous plant and insect pests and are well-known to synthesize a variety of bioactive secondary metabolites. We hypothesized that bacteria isolated from agricultural soil would be effective antagonists of soilborne fungal pathogens. Here, we show that the Delaware soil isolate Bacillus velezensis strain S4 has in vitro activity against soilborne and foliar plant pathogenic fungi, including two with a large host range, and one oomycete. Further, this strain shows putative protease and cellulase activity, consistent with our prior finding that the genome of this organism is highly enriched in antifungal and antimicrobial biosynthetic gene clusters. We demonstrate that this bacterium causes changes to the fungal and oomycete hyphae at the inhibition zone, with some of the hyphae forming bubble-like structures and irregular branching. We tested strain S4 against Magnaporthe oryzae spores, which typically form germ tubes and penetration structures called appressoria, on the surface of the leaf. Our results suggest that after 12 hours of incubation with the bacterium, fungal spores form germ tubes, but instead of producing appressoria, they appear to form rounded, bubble-like structures. Future work will investigate whether a single antifungal molecule induces all these effects, or if they are the result of a combination of bacterially produced antimicrobials.

show abstract

Complete Genome Sequence of Bacillus velezensis Strain S4, Isolated from Biochar-Treated Soil

Abstract: Here, we report the complete genome sequence of Bacillus velezensis strain S4, which was isolated from biochar-amended agricultural soil collected in Smyrna, Delaware. The genome is 4.07 Mbp, encodes 3,918 predicted proteins, and has a GC content of 46.4%.

Cited by 3 publications

References 10 publications

Genetic and Phenotypic Characterization of Bacillus velezensis Strain BV379 for Human Probiotic Applications

Genetic and Phenotypic Characterization of Bacillus velezensis Strain BV379 for Human Probiotic Applications

Microbiomes of commercially-available pine nuts and sesame seeds

A Bacillus velezensis strain shows antimicrobial activity against soilborne and foliar fungi and oomycetes

Contact Info

Product

Resources

About