Complete genome sequence of Bougainvillea chlorotic vein banding virus in Bougainvillea spectabilis from Brazil

Abstract

We report the first full-length genome of a Bougainvillea chlorotic vein banding virus, isolate BCVBV-UNB-01, causing leaf yellowing in a Bougainvillea spectabilis from Brasília, Brazil. The genome, recovered using high throughput sequencing (Illumina HiSeq) and Sanger, consisted of 8665 nucleotides and four typical badnavirus ORFs: ORF I with 456 nt in length, ORF II with 432 nt, ORF III with 6636 nt and ORF IV with 594 nt. Phylogenetic analysis using Bayesian inference of the 1228 bp of the RT + RNase H region along other 35 badnaviruses clustered isolate BCVBV-UNB-01 with BCVBV accession (NC011592) from Taiwan. The accurately aligned region of 1228 bp using Sequence Demarcation Tool (SDT) software, confirmed this result, evidencing a robust identification. In addition, further studies involving biological characteristics, distribution and diversity of BCVBV in Brazil will be conducted in an attempt for better understanding the role of interactions among virus, plant and vector.

Emerging and Re-Emerging Diseases Caused by Badnaviruses

“…Badnaviruses are also reported to infect several ornamentals, medicinal herbs, fruits, trees, and weeds [ 154 , 155 , 156 , 157 , 158 , 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 , 181 , 182 , 183 , 184 , 185 , 186 , 187 , 188 , 189 ]. Birch leaf roll-associated virus, pagoda yellow mosaic-associated virus, and mulberry badnavirus 1 are known to infect different tree crops ( Table 1 ) [ 156 , 157 , 158 ].…”

“…Birch leaf roll-associated virus, pagoda yellow mosaic-associated virus, and mulberry badnavirus 1 are known to infect different tree crops ( Table 1 ) [ 156 , 157 , 158 ]. Similarly, aglaonema bacilliform virus, aucuba ringspot virus, bougainvillea spectabilis chlorotic vein-banding virus, camellia lemon glow virus, canna yellow mottle-associated virus, canna yellow mottle virus, codonopsis vein-clearing virus, epiphyllum mottle-associated virus, ivy ringspot-associated virus, kalanchoë top-spotting virus, spiraea yellow leaf spot virus, wisteria badnavirus 1, and rubus yellow net virus are reported to infect different ornamentals ( Table 1 ) [ 154 , 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 170 , 171 , 172 , 174 , 175 , 176 ], while cycad leaf necrosis virus infects the gymnosperm Zamia fischeri [ 177 ]. Viruses such as blackberry virus F, chestnut mosaic virus, fig badnavirus 1, gooseberry vein banding-associated virus, and jujube mosaic-associated virus infect different fruit crops [ 155 , 178 , 179 , 180 , 181 , 182 , 183 , 184 , 185 ], while polyscias mosaic virus and green Sichuan pepper vein clearing-associated virus infect medicinal and spice crops [ 186 , 187 ].…”