Complete Genome Sequence of Campylobacter hepaticus RBCL71delta, Associated with Spotty Liver Disease in Organic Pasture-Raised Laying Hens in Georgia, USA

Becerra, Roel; Ienes-Lima, Julia; Nicholds, Jenny

doi:10.1128/mra.01309-22

Cited by 1 publication

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One C. hepaticus genome was closed into a chromosome (RBCL71delta), and the other three genomes were assembled into contigs ( Becerra et al, 2023a ). The genome from C. hepaticus RBCL71delta has a GC content of 28.03% and consists of 1,516,079 base pairs.…”

Section: Resultsmentioning

confidence: 99%

“…To date, the genome sequences of C. hepaticus that are available for analysis and study are limited, which presents a challenge for identification of virulence traits and potential targets for control and vaccine development. Here, we analyzed the genomes of four C. hepaticus isolated from our study ( Becerra et al, 2023a ) and those available in NCBI and other genome databases, using the C. hepaticus type strain HV10 ( Van et al, 2016 ) as reference genome. We also included an additional series of Campylobacter genomes from the genus that are closely related to compare our sequenced genomes with those of other Campylobacter species.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative genomic analysis of Campylobacter hepaticus genomes associated with spotty liver disease, Georgia, United States

Ienes-Lima

Becerra

2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Campylobacter hepaticus has re-emerged as an important cause of disease in egg laying birds worldwide, resulting in morbidity, mortality, and significant losses in eggs for the breeding and table egg laying industries. Although birds may appear asymptomatic, the disease is characterized by spots on the liver of birds and histopathological analysis reveals multifocal fibrogranulocytic necrotizing hepatitis microscopically. The re-emergence of C. hepaticus may be linked with housing practices as the disease appears more prevalent in pasture raised birds with outside exposure. Here we describe, the whole genome sequences and comparative analysis of four C. hepaticus genomes associated with an outbreak on pasture raised breeders from a farm in Georgia, United States. All four genomes were relatively similar in size and virulence genes harbored. Using these genomes, comparison with current C. hepaticus genomes available in NCBI and other databases and other members of the Campylobacter species was carried out. Using current tools available, virulence gene factor content was compared, and it was found that different tools lead to different numbers of factors identified. The four genomes from this study were relatively similar to C. hepaticus HV10 the type strain from Australia but differed from the other sequenced US strains from Iowa and Florida. C. hepaticus was found to have an overall lower gene content for genes associated with virulence and iron acquisition compared to other Campylobacter genomes and appears to cluster differently than UK genomes on phylogenetic analysis, suggesting the emergence of two lineages of C. hepaticus. This analysis provides valuable insight into the emerging pathogen C. hepaticus, its virulence factors and traits associated with disease in poultry production in the US, potentially providing insight into targets for its control and treatment for laying birds. Our analysis also confirms genes associated with iron acquisition are limited and the presence of the multidrug efflux pump CmeABC in C. hepaticus which may promote survival and persistence in the host niche – the chicken liver/bile. One unique aspect of this study was the finding of a close genetic relationship between C. hepaticus and Campylobacter fetus species and evidence of genome reduction in relation to host niche specificity.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%