Complete Genome Sequence of Cellulase-Producing Microbulbifer sp. Strain GL-2, Isolated from Marine Fish Intestine

Abstract

Microbulbifer sp. strain GL-2 was isolated from the intestine of a teleost, Girella melanichthys. Here, we report the complete genome sequence of this strain, which produces cellulase(s). Twelve cellulase candidate genes were found on the chromosome.

Cellulolytic enzymes in <i>Microbulbifer</i> sp. Strain GL-2, a marine fish intestinal bacterium, with emphasis on endo-1,4-β-glucanases Cel5A and Cel8

“…Strain GL-2, the bacterium used in the present study, was isolated from the intestine of a blackfish (Girella melanichthys) (Tanaka et al, 2021). The whole-genome sequence of this strain has been deposited in DDBJ/EMBL/GenBank as Accession Number AP019807 (Sugimoto et al, 2020); a circular map of the genome is shown in Fig. S1.…”

“…Given that we have previously reported the whole-genome sequence of GL-2 (Sugimoto et al, 2020), we sought, in the present study, to identify and characterize cellulases encoded by this genome, including the expression of the corresponding genes and the enzymatic properties of the resulting recombinant proteins. We report here the cellulase-encoding genes of the GL-2 genome, expression of these genes, and characterization of the encoded enzymes, including the effects of various conditions and saccharides on the expression and activities of these proteins.…”