Complete Genome Sequence of Corynebacterium falsenii DSM 44353 To Study the Evolution of
            <i>Corynebacterium</i>
            Cluster 3 Species

Glaub, Alina; Bomholt, Christina; Gravermann, Kerstin; Brinkrolf, Karina; Albersmeier, Andreas; Rückert, Christian; Tauch, Andreas

doi:10.1128/genomea.00158-14

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…According to QUAST version 4.6.0 and CheckM version 1.0.8, the quality of the genomes is comparable to the only other assembly ( C . bovis DSM 20582 T , 98.1% completeness) available for the species with a range of 93.1–99.1% (97.8 ± 0.36%) estimated completeness from 585 single-copy gene markers in the Corynebacterium genus (n = 80 genomes) [45–47]. The cumulative length of the genome was comparable between isolates including the species Type strain with an average of 2.53 ± 0.03 Mbp, as well as the characteristic high G+C content (72.44 ± 0.09%) reported for this species.…”

Section: Resultsmentioning

confidence: 99%

“…Corynebacterium falsenii was first isolated from human blood cultures and cerebrospinal fluid in 1991 and 1995, respectively [47]. The species has also been isolated from an infant with bacteremia where C .…”

Section: Resultsmentioning

confidence: 99%

“…falsenii DSM 44353 T included a circular chromosome of 2,677,607 bp (63.18% G+C content) and a circular corynephage ɸCFAL8171I genome of 42,009 bp (61.74% G+C content) [47]. An identical linear copy of ɸCFAL8171I was present in the chromosome as a prophage, which suggested, based upon the circularized phage genome, that this corynephage had entered a lytic cycle in a subpopulation of the culture used to prepare the genomic DNA for WGS [47]. For the purpose of our study, the completed genome of the type strain of C .…”

Section: Resultsmentioning

confidence: 99%

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Genotypic differences between strains of the opportunistic pathogen Corynebacterium bovis isolated from humans, cows, and rodents

et al. 2018

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Corynebacterium bovis is an opportunistic bacterial pathogen shown to cause eye and prosthetic joint infections as well as abscesses in humans, mastitis in dairy cattle, and skin disease in laboratory mice and rats. Little is known about the genetic characteristics and genomic diversity of C. bovis because only a single draft genome is available for the species. The overall aim of this study was to sequence and compare the genome of C. bovis isolates obtained from different species, locations, and time points. Whole-genome sequencing was conducted on 20 C. bovis isolates (six human, four bovine, nine mouse and one rat) using the Illumina MiSeq platform and submitted to various comparative analysis tools. Sequencing generated high-quality contigs (over 2.53 Mbp) that were comparable to the only reported assembly using C. bovis DSM 20582T (97.8 ± 0.36% completeness). The number of protein-coding DNA sequences (2,174 ± 12.4) was similar among all isolates. A Corynebacterium genus neighbor-joining tree was created, which revealed Corynebacterium falsenii as the nearest neighbor to C. bovis (95.87% similarity), although the reciprocal comparison shows Corynebacterium jeikeium as closest neighbor to C. falsenii. Interestingly, the average nucleotide identity demonstrated that the C. bovis isolates clustered by host, with human and bovine isolates clustering together, and the mouse and rat isolates forming a separate group. The average number of genomic islands and putative virulence factors were significantly higher (p<0.001) in the mouse and rat isolates as compared to human/bovine isolates. Corynebacterium bovis’ pan-genome contained a total of 3,067 genes of which 1,354 represented core genes. The known core genes of all isolates were primarily related to ‘‘metabolism” and ‘‘information storage/processing.” However, most genes were classified as ‘‘function unknown” or “unclassified”. Surprisingly, no intact prophages were found in any isolate; however, almost all isolates had at least one complete CRISPR-Cas system.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Genotypic differences between strains of the opportunistic pathogen Corynebacterium bovis isolated from humans, cows, and rodents

et al. 2018

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“…For example, C. falsenii contains the corynephage ɸCFAL8171I in both circular (extrachromosomal) and linear (chromosomally integrated) states. 24 Evidence is available-albeit sparse-to show that not all bacteria can be infected by phages. 32 Several antiphage systems found in bacteria have been acquired through evolution or horizontal transfer.…”

Section: Discussionmentioning

confidence: 99%

Searching for a Bacteriophage Lysin to Treat Corynebacterium bovis in Immunocompromised Mice

et al. 2020

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Corynebacterium bovis is the causative agent of Corynebacterium-associated hyperkeratosis in immunocompromised mice. The resulting skin pathology can be profound and can be associated with severe wasting, making the animals unsuitable for research. Although the administration of antibiotics is effective in resolving clinical symptoms, antibiotics do not eradicate the offending bacterium. Furthermore, antibiotic use may be contraindicated as it can affect tumor growth and is associated with Clostridioides difficile enterotoxemia in highly immunocompromised murine strains. Lysins, which are lytic enzymes obtained from bacteriophages, are novel antimicrobial agents for treating bacterial diseases. The advantage of lysins are its target specificity, with minimal off-target complications that could affect the host or the biology of the engrafted tumor. The aim of this study was to identify lysins active against C. bovis. Chemical activation of latent prophages by using mitomycin C in 3 C. bovis isolates did not cause bacteriophage induction as determined through plaque assays and transmission electron microscopy. As an alternative approach, 8 lysins associated with other bacterial species, including those from the closely related species C. falsenii, were tested for their lytic action against C. bovis but were unsuccessful. These findings were congruentwith the previously reported genomic analysis of 21 C. bovis isolates, which failed to reveal bacteriophage sequences by usingthe PHAST and PHASTER web server tools. From these results, we suggest C. bovis is among those rare bacterial species devoid of lysogenic bacteriophages, thus making the identification of C. bovis-specific lysins more challenging. However, C. bovis may be a useful model organism for studying the effects of antiphage systems.

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Complete genome sequence of Corynebacterium vitaeruminis DSM 20294T, isolated from the cow rumen as a vitamin B producer

Al-Dilaimi

Albersmeier

Kalinowski

et al. 2014

Journal of Biotechnology

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Complete Genome Sequence of Corynebacterium falsenii DSM 44353 To Study the Evolution of Corynebacterium Cluster 3 Species

Cited by 5 publications

References 14 publications

Genotypic differences between strains of the opportunistic pathogen Corynebacterium bovis isolated from humans, cows, and rodents

Genotypic differences between strains of the opportunistic pathogen Corynebacterium bovis isolated from humans, cows, and rodents

Searching for a Bacteriophage Lysin to Treat Corynebacterium bovis in Immunocompromised Mice

Complete genome sequence of Corynebacterium vitaeruminis DSM 20294T, isolated from the cow rumen as a vitamin B producer

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