Complete Genome Sequence of Dolosigranulum pigrum from a Patient with Interstitial Lung Disease Using Single-Molecule Real-Time Sequencing Technology

Mukhopadhyay, Rituparna; Joaquin, Joselita; Hogue, Robin; Fitzgerald, Susan; Jospin, Guillaume; Mars, Kristin; Eisen, Jonathan A.; Chaturvedi, Vishnu

doi:10.1128/genomea.00317-17

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…Nasopharygneal Laclaire et al [10] ? Nasopharyngeal Mukhopadhyay et al [11] 82, M ILD ILD -Lin et al [12] 76, M Cholecystitis --Lecuyer et al [13] 73 [19]. It seems likely that in the case of our patient, he contracted D. Pigrum bacteremia from previously licking his needles.…”

Section: Coinfecting Pathogenmentioning

confidence: 76%

“…The preponderance of case reports documenting pathology have involved focal eye diseases such as conjunctivitis, keratitis and corneal abscess [1,[6][7][8][9][10]. The pathological spectrum extends to other critical illnesses: synovitis, sinusitis, pneumonia, cholecystitis and septicemia, however it has only rarely been associated with pre-existing immunocompromised states per se (Cystic fibrosis, HIV, Rheumatoid arthritis, Interstitial lung disease, chronic disease, extremes of age) [5,[11][12][13][14][15][16]. Here, we present the case of D. Pigrum bacteremia in an injecting drug user (IDU), in constellation with immunocompromise from comorbid chronic HCV, fungal endophthalmitis and MRSA endocarditis.…”

Section: Introductionmentioning

confidence: 99%

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Dolosigranulum Pigrum Bacteremia in an Injecting Drug User Associated with Methicillin Resistant Staphylococcus Aureus Endocarditis and Fungal Endophthalmitis

Paul O’Shea

2024

IJCCR

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Dolosigranulum pigrum is a commensal bacterium of the upper respiratory tract. We present a case of D. Pigrum bacteremia, identified incidentally in a patient with a history of injecting drug use (IDU), chronic hepatitis C (HCV) and partially treated methicillin resistant staphylococcus aureus (MRSA) endocarditis, who presented with acute monocular vision loss. Workup was consistent with unilateral fungal endophthalmitis. The patient was initially treated with intravenous Voriconazole, Ceftriaxone and linezolid for 4 weeks with resolution of his symptoms. This case highlights the emerging role of D. Pigrum as a pathogen in the immune compromised and specifically in the IDU population. This case further illustrates that although identification of fungal growth in vitreous fluid may include culture and PCR analysis, fungal endophthalmitis remains a clinical diagnosis and that fungal cultures have lower sensitivity.

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Section: Coinfecting Pathogenmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Dolosigranulum Pigrum Bacteremia in an Injecting Drug User Associated with Methicillin Resistant Staphylococcus Aureus Endocarditis and Fungal Endophthalmitis

Paul O’Shea

2024

IJCCR

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“…Our metagenomic data identified that D. pigrum was the only species that remained group-discriminating after adjustment for HRV status. This bacterium was first described as a new lineage of lactic acid bacterium ( Aguirre et al, 1993 ), and its whole-genome sequence was announced in 2017 ( Mukhopadhyay et al, 2017 ). Recent studies revealed an inverse correlation in RAs between D. pigrum and Staphylococcus aureus , a pathobiont in the respiratory tract ( Liu et al, 2015 ; Escapa et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Shotgun Sequencing for Nasopharyngeal Microbiome in Pre-school Children With Recurrent Wheezing

et al. 2022

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Microbiome mediates early life immune deviation in asthma development. Recurrent wheeze (RW) in pre-school years is a risk factor for asthma diagnosis in school-age children. Dysbiosis exists in asthmatic airways, while its origin in pre-school years and relationship to RW is not clearly defined. This study investigated metagenomics of nasopharyngeal microbiome in pre-school children with RW. We applied whole-genome shotgun sequencing and human rhinovirus (HRV) detection on nasopharyngeal samples collected from three groups of pre-school children: (i) RW group: 16 children at-risk for asthma who were hospitalized for RW, (ii) inpatient control (IC): 18 subjects admitted for upper respiratory infection, and (iii) community control (CC): 36 children without respiratory syndromes. Sequence reads were analyzed by MetaPhlAn2 and HUMAnN2 algorithm for taxonomic and functional identification. Linear discriminant analysis effect size (LEfSe) analysis was used to identify discriminative features. We identified that Moraxella catarrhalis and Dolosigranulum pigrum were predominant species in nasopharynx. RW had lower alpha diversity (Shannon diversity index) than CC (0.48 vs. 1.07; Padj = 0.039), characterized by predominant Proteobacteria. LEfSe analysis revealed D. pigrum was the only discriminative species across groups (LDA = 5.57, P = 0.002), with its relative abundance in RW, IC, and CC being 9.6, 14.2, and 37.3%, respectively (P < 0.05). LEfSe identified five (ribo)nucleotides biosynthesis pathways to be group discriminating. Adjusting for HRV status, pre-school children with RW have lower nasopharyngeal biodiversity, which is associated with Proteobacteria predominance and lower abundance of D. pigrum. Along with discriminative pathways found in RW and CC, these microbial biomarkers help to understand RW pathogenesis.

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Septic shock and death due to <i>Dolosigranulum pigrum</i> bacteremia: a case report

Hakoda,

Ishiwata,

Shimada

et al. 2024

Jpn J Infect Dis

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Complete Genome Sequence of Dolosigranulum pigrum from a Patient with Interstitial Lung Disease Using Single-Molecule Real-Time Sequencing Technology

Cited by 3 publications

References 7 publications

Dolosigranulum Pigrum Bacteremia in an Injecting Drug User Associated with Methicillin Resistant Staphylococcus Aureus Endocarditis and Fungal Endophthalmitis

Dolosigranulum Pigrum Bacteremia in an Injecting Drug User Associated with Methicillin Resistant Staphylococcus Aureus Endocarditis and Fungal Endophthalmitis

Whole-Genome Shotgun Sequencing for Nasopharyngeal Microbiome in Pre-school Children With Recurrent Wheezing

Septic shock and death due to <i>Dolosigranulum pigrum</i> bacteremia: a case report

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