Complete Genome Sequence of
 Enterobacter cloacae
 subsp.
 cloacae
 Type Strain ATCC 13047

Ren, Yan; Ren, Yi; Zhou, Zhemin; Guo, Xi; Li, Yayue; Feng, Lu; Wang, Lei

doi:10.1128/jb.00067-10

Cited by 116 publications

(94 citation statements)

References 15 publications

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“…While the expression of silCFBAGP is thought to be governed by the twocomponent membrane sensor and transcriptional responder SilRS; the expression of silE, just like homolog pcoE, is thought to be regulated/ co-regulated by the Cus system (Zimmerman et al 2012), and therefore, SilE perhaps is involved in the activation of regulators SilRS (proposed by dotted arrow) 2015) and Enterobacter cloacae subsp. cloacae strain ATCC 13047 (Ren et al 2010). For E. coli and Enterobacter strains, the copper pathogenicity island was identified in close vicinity to Tn7-like transposons with tnsABCD being present (Peters et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Hao

Lüthje

Qin

et al. 2015

Appl Microbiol Biotechnol

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The presence of metal resistance determinants in bacteria usually is attributed to geological or anthropogenic metal contamination in different environments or associated with the use of antimicrobial metals in human healthcare or in agriculture. While this is certainly true, we hypothesize that protozoan predation and macrophage killing are also responsible for selection of copper/zinc resistance genes in bacteria. In this review, we outline evidence supporting this hypothesis, as well as highlight the correlation between metal resistance and pathogenicity in bacteria. In addition, we introduce and characterize the Bcopper pathogenicity island^identified in Escherichia coli and Salmonella strains isolated from copper-and zinc-fed Danish pigs.

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Section: Introductionmentioning

confidence: 99%

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Hao

Lüthje

Qin

et al. 2015

Appl Microbiol Biotechnol

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“…However, the upstream region of Tn1331 (⌬tnpA) in pBK30661 was truncated by an 8-kb nickel resistance operon (nic operon), which showed 98% nucleotide similarity to that of plasmid pECL_A from Enterobacter cloacae subsp. cloacae strain ATCC 13047 (24). The insertion of the nic operon also deleted the corresponding 5-bp target sequence (AG AAC) of Tn4401d in pBK30661, leaving a 5-bp unique sequence (AGAGA) adjacent to the upstream IR sequence of Tn4401d (Fig.…”

Section: Microbiological Characterization Of Isolates Bk30661 and Bk3mentioning

confidence: 99%

Molecular Survey of the Dissemination of Two bla _KPC -Harboring IncFIA Plasmids in New Jersey and New York Hospitals

Chen

Chavda

Melano

et al. 2014

Antimicrob Agents Chemother

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i Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae strains have spread worldwide and become a major threat in health care facilities. Transmission of bla KPC , the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide sequences of two novel bla KPC-3 -harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length, with a mosaic plasmid structure; it exhibits homologies to several other plasmids but lacks the plasmid transfer operon (tra) and the origin of transfer (oriT) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrated plasmid structure, comprising a 72-kb element that highly resembles pBK30661 (>99.9% nucleotide identities) and an extra 68-kb element that harbors tra and oriT. A PCR scheme was designed to detect the distribution of bla KPC -harboring IncFIA (pBK30661-like and pBK30683-like) plasmids in a collection of clinical Enterobacteriaceae isolates from 10 hospitals in New Jersey and New York. KPC-harboring IncFIA plasmids were found in 20% of 491 K. pneumoniae isolates, and all carried bla KPC-3 . pBK30661-like plasmids were identified mainly in the epidemic sequence type 258 (ST258) K. pneumoniae clone, while pBK30683-like plasmids were widely distributed in ST258 and other K. pneumoniae sequence types and among non-K. pneumoniae Enterobacteriaceae species. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination of bla KPC -harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and to decipher the origins of pBK30661-like and pBK30683-like plasmids.

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“…Resistance to carbapenems including carbapenemase production is emerging, creating difficulties in the management of life-threatening infections [1]. Amongst the enterobacteriaceae family, Enterobacter species cause a number of hospital acquired infections [2] and Enterobacter cloacae is the most resistant pathogen [3]. The dominant genes contributing to drug resistance in E. cloacae are the plasmid-mediated serine extended spectrum β-lactamase (ESBL) bla CTX-M ; the serine carbapenemase bla KPC and the metallo-β-lacatamases (MBLs) bla NDM , bla IMP and bla VIM [4] [5].…”

Section: Introductionmentioning

confidence: 99%

Emerging Carbapenem-Resistant Enterobacter cloacae Producing OXA-48-, VIM- and IMP-Type-β-Lactamases in Eastern Cape Hospitals in South Africa

Singh-Moodley¹,

Ekermans²,

Perovic³

2015

OJMM

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Introduction: Enterobacter cloacae strains have been isolated from Eastern Cape hospitalised patients. Methodology: We have molecularly characterised blaOXA-48-, blaIMP-and blaVIM-expressing E. cloacae isolates demonstrating resistance to carbapenems from five hospitals by multilocus sequence typing. Organism identification and antimicrobial susceptibility testing was done using automated systems and the isolates were screened for carbapenemases using either conventional or real-time PCR and then typed using multilocus sequence typing. Further characterisation of IMPtype-producing E. cloacae isolates, an unusual occurrence in South Africa, was performed by pulsedfield gel electrophoresis. Results and Conclusion: Twenty-five E. cloacae isolates from 24 patients were investigated. Eighteen (72%) isolates harboured either one of the following genes: blaIMP, blaVIM or blaOXA-48. Multilocus sequence typing data and pulsed-field gel electrophoresis showed that several strains from the same geographical region and hospitals were genetically related.

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Complete Genome Sequence of Enterobacter cloacae subsp. cloacae Type Strain ATCC 13047

Cited by 116 publications

References 15 publications

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Molecular Survey of the Dissemination of Two bla _KPC -Harboring IncFIA Plasmids in New Jersey and New York Hospitals

Emerging Carbapenem-Resistant <i>Enterobacter cloacae</i> Producing OXA-48-, VIM- and IMP-Type-<i>β</i>-Lactamases in Eastern Cape Hospitals in South Africa

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Complete Genome Sequence of Enterobacter cloacae subsp. cloacae Type Strain ATCC 13047

Cited by 116 publications

References 15 publications

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Molecular Survey of the Dissemination of Two bla KPC -Harboring IncFIA Plasmids in New Jersey and New York Hospitals

Emerging Carbapenem-Resistant &lt;i&gt;Enterobacter cloacae&lt;/i&gt; Producing OXA-48-, VIM- and IMP-Type-&lt;i&gt;β&lt;/i&gt;-Lactamases in Eastern Cape Hospitals in South Africa

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Molecular Survey of the Dissemination of Two bla _KPC -Harboring IncFIA Plasmids in New Jersey and New York Hospitals

Emerging Carbapenem-Resistant <i>Enterobacter cloacae</i> Producing OXA-48-, VIM- and IMP-Type-<i>β</i>-Lactamases in Eastern Cape Hospitals in South Africa