Complete Genome Sequence of Neisseria meningitidis Serogroup B Strain MC58

Abstract

The 2,272,351-base pair genome of Neisseria meningitidis strain MC58 (serogroup B), a causative agent of meningitis and septicemia, contains 2158 predicted coding regions, 1158 (53.7%) of which were assigned a biological role. Three major islands of horizontal DNA transfer were identified; two of these contain genes encoding proteins involved in pathogenicity, and the third island contains coding sequences only for hypothetical proteins. Insights into the commensal and virulence behavior of N. meningitidis can be gleaned from the genome, in which sequences for structural proteins of the pilus are clustered and several coding regions unique to serogroup B capsular polysaccharide synthesis can be identified. Finally, N. meningitidis contains more genes that undergo phase variation than any pathogen studied to date, a mechanism that controls their expression and contributes to the evasion of the host immune system.

Genomic Exploration of the Hemiascomycetous Yeasts: 3. Methods and strategies used for sequence analysis and annotation

“…the sequences of Neisseria meningitidis [57], D. radiodurans [58], Drosophila melanogaster [59], chromosomes 2 and 4 from Arabidopsis thaliana [60,61], chromosomes 2 and 3 from Plasmodium falciparum [62,63], and chromosomes 21 and 22 from Homo sapiens [64,65] that were recently reported. For the few cases of homologies to these organisms, refer to [37^49].…”

Toward a Genome-Wide Systems Biology Analysis of Host-Pathogen Interactions in Group A Streptococcus

“…[73][74][75][76] The basic concept behind the reverse vaccinology approach is to use the complete genome sequence of a pathogen to identify a large number of novel protective antigens, express each candidate in recombinant form, and test each protein in one or more assays for capacity to confer immunological protection. Potential candidates are initially identified by computer analysis of the bacterial genome sequence with algorithms that predict secreted or surface-exposed proteins.…”

“…Pioneering studies were performed using Neisseria meningitidis, an important cause of sepsis and meningitis worldwide. 73,74 The complete genome sequence of a serogroup B strain of this pathogen was determined, 344 candidate proteins were overexpressed in Escherichia coli, and 28 novel proteins were ultimately identified as protective antigens. 74 Subsequently, a similar strategy, with some improvements, was used to identify novel protective antigens against group B Streptococcus 76 and GAS.…”

Lossless Filter for Finding Long Multiple Approximate Repetitions Using a New Data Structure, the Bi-factor Array

“…The last DNA sequence is the genomic sequence of the Neisseria meningitidis strain MC58. Neisseria genomes are known for the abundance and diversity of their repetitive DNA in terms of size and structure [6]. The size of the repeated elements range from 10 bases to more than 2000 bases, and their number, depending on the type of the repeated element, may reach more than 200 copies.…”