Complete Genome Sequence of IME13, a Stenotrophomonas maltophilia Bacteriophage with Large Burst Size and Unique Plaque Polymorphism

Fan, Huahao; Huang, Yucong; Mi, Zhiqiang; X, Yin; Wang, L.; Zhang, Z.; An, Xiaoping; Chen, J.; Tong, Yigang

doi:10.1128/jvi.01908-12

Cited by 27 publications

(30 citation statements)

References 10 publications

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“…Bacteriophage AXL3 (vB_SmaS-AXL_3) was isolated using clinical S. maltophilia strain D1585 from soil collected at the Patrick Seymour Alpine Garden at the University of Alberta Botanic Gardens. S. maltophilia phages have previously been isolated from soil and rhizosphere samples [ 10 , 11 , 17 , 18 , 19 ]. This phage forms small plaques 0.78 ± 0.12 mm in diameter with clear borders after 16 h incubation on its main host, D1585.…”

Section: Resultsmentioning

confidence: 99%

“…The use of phages for human therapy requires extensive phage characterization, including host range analysis, genomic characterization and identification of potential moron genes, lifestyle determination and identification of cell surface receptors. In the last two decades, numerous phages against S. maltophilia have been isolated and characterized to various extents for their therapeutic potential [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. Herein we describe the isolation and characterization of a novel S. maltophilia phage, AXL3, unrelated to any phages currently sequenced in the NCBI database.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

McCutcheon

Lin

Dennis

2020

IJMS

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The rapid increase in the number of worldwide human infections caused by the extremely antibiotic resistant bacterial pathogen Stenotrophomonas maltophilia is cause for concern. An alternative treatment solution in the post-antibiotic era is phage therapy, the use of bacteriophages to selectively kill bacterial pathogens. In this study, the novel bacteriophage AXL3 (vB_SmaS-AXL_3) was isolated from soil and characterized. Host range analysis using a panel of 29 clinical S. maltophilia isolates shows successful infection of five isolates and electron microscopy indicates that AXL3 is a member of the Siphoviridae family. Complete genome sequencing and analysis reveals a 47.5 kb genome predicted to encode 65 proteins. Functionality testing suggests AXL3 is a virulent phage and results show that AXL3 uses the type IV pilus, a virulence factor on the cell surface, as its receptor across its host range. This research identifies a novel virulent phage and characterization suggests that AXL3 is a promising phage therapy candidate, with future research examining modification through genetic engineering to broaden its host range.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

McCutcheon

Lin

Dennis

2020

IJMS

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“…Unexpectedly, tail sheath proteins of maltocins are more evolutionally related to that of phage PPpW‐3 than those of S. maltophilia phages. Since the number of known Myoviridae phage genomes of S. maltophilia is very limited (Chen et al ; Fan et al ; Lee et al ; Peters et al ), whether the origin of maltocins relates to respective S. maltophilia phages needs to be explored further.…”

Section: Discussionmentioning

confidence: 99%

Characterization of maltocin S16, a phage tail‐like bacteriocin with antibacterial activity againstStenotrophomonas maltophiliaandEscherichia coli

et al. 2019

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Aims To determine the characteristics of the novel phage tail‐like bacteriocin (PTLB) produced by Stenotrophomonas maltophilia S16. Methods and Results A screen to identify novel bacteriocins from a panel of 86 S. maltophilia strains was performed. We found that 62 tested S. maltophilia strains were sensitive to a PTLB, designated maltocin S16. In addition, 8 of 14 tested Escherichia coli strains were also susceptible to maltocin S16. Minimum inhibitory concentration determination confirmed that maltocin S16 had rather high bactericidal activity against both S. maltophilia and E. coli. Maltocin S16 was resistant to trypsin and proteinase K. Furthermore, lipopolysaccharide was found to be involved in the adsorption of maltocin S16. The gene cluster of maltocin S16 was consisted of 23 putative open reading frames. Phylogenetic analysis indicated that maltocin S16 represents a new class of PTLB in S. maltophilia. Conclusion Maltocin S16 was a novel PTLB with broad‐spectrum and high bactericidal activity against S. maltophilia and E. coli. Significance and Impact of the Study Stenotrophomonas maltophilia is a multidrug‐resistant opportunistic pathogen with increasing contributions to infectious disease morbidity and mortality. Maltocin S16 could be a promising alternative for antibiotics against S. maltophilia and E. coli infections.

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“…Recent research has led to the isolation and characterization of several different S. maltophilia phages, including a jumbo phage phiSMA5 (with a genome of approximately 250 kb in length, [ 22 ]), a filamentous phage phiSMA9 with a genome size of 6.9 kb encoding only seven genes, but one of these being a zot toxin [ 23 ], a virulent phage Smp14 that exhibits homology to phage T4 [ 24 ], a lytic phage IME13 with an unusually large burst size [ 25 ], a T7-like phage IME15 specific to S. maltophilia [ 26 ], a P2-like phage Smp131 whose genome exhibits sequence homology to prophages in Xanthomonas species [ 27 ], and three other novel, small filamentous phages phiSMA 6, phiSMA7 and phiSHP1 [ 28 , 29 ]. Twenty-two phages specific for different Stenotrophomonas species, including the well-characterized temperate phage S1, have also recently been isolated [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity

et al. 2015

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BackgroundA rapid worldwide increase in the number of human infections caused by the extremely antibiotic resistant bacterium Stenotrophomonas maltophilia is prompting alarm. One potential treatment solution to the current antibiotic resistance dilemma is “phage therapy”, the clinical application of bacteriophages to selectively kill bacteria.ResultsTowards that end, phages DLP1 and DLP2 (vB_SmaS-DLP_1 and vB_SmaS-DLP_2, respectively) were isolated against S. maltophilia strain D1585. Host range analysis for each phage was conducted using 27 clinical S. maltophilia isolates and 11 Pseudomonas aeruginosa strains. Both phages exhibit unusually broad host ranges capable of infecting bacteria across taxonomic orders. Transmission electron microscopy of the phage DLP1 and DLP2 morphology reveals that they belong to the Siphoviridae family of bacteriophages. Restriction fragment length polymorphism analysis and complete genome sequencing and analysis indicates that phages DLP1 and DLP2 are closely related but different phages, sharing 96.7 % identity over 97.2 % of their genomes. These two phages are also related to P. aeruginosa phages vB_Pae-Kakheti_25 (PA25), PA73, and vB_PaeS_SCH_Ab26 (Ab26) and more distantly related to Burkholderia cepacia complex phage KL1, which together make up a taxonomic sub-family. Phages DLP1 and DLP2 exhibited significant differences in host ranges and growth kinetics.ConclusionsThe isolation and characterization of phages able to infect two completely different species of bacteria is an exciting discovery, as phages typically can only infect related bacterial species, and rarely infect bacteria across taxonomic families, let alone across taxonomic orders.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1848-y) contains supplementary material, which is available to authorized users.

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Complete Genome Sequence of IME13, a Stenotrophomonas maltophilia Bacteriophage with Large Burst Size and Unique Plaque Polymorphism

Cited by 27 publications

References 10 publications

Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

Characterization of maltocin S16, a phage tail‐like bacteriocin with antibacterial activity againstStenotrophomonas maltophiliaandEscherichia coli

The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity

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