Complete Genome Sequence of Klebsiella oxytoca KCTC 1686, Used in Production of 2,3-Butanediol

Shin, Sang Heum; Kim, Sewhan; Kim, Jae Young; Lee, Soojin; Um, Youngsoon; Oh, Min Kyu; Kim, Young-Rok; Lee, Jinwon; Yang, Kap Seok

doi:10.1128/jb.00026-12

Cited by 27 publications

(14 citation statements)

References 12 publications

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“…2013; Chen et al. 2013), and one environmental isolate from South Korea (Shin et al. 2012), one isolate recovered from panda faeces in China (Jiang et al.…”

Section: Resultsmentioning

confidence: 99%

Population Structure of Multidrug-Resistant Klebsiella oxytoca within Hospitals across the United Kingdom and Ireland Identifies Sharing of Virulence and Resistance Genes with K. pneumoniae

Moradigaravand

Martin

Peacock

et al. 2017

Genome Biology and Evolution

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Klebsiella oxytoca, a member of the Enterobacteriaceae, is a gram-negative pathogenic bacterium of environmental origin, which can cause infection in healthcare settings. Outbreaks of multidrug-resistant K. oxytoca infection have been increasingly reported in hospitalized patients. Despite the growing importance of this pathogen, there is limited knowledge about the population structure and epidemiology of antimicrobial resistant K. oxytoca. We investigated the population structure and genomic basis of antimicrobial resistance of 41 multidrug resistant K. oxytoca isolates recovered from bloodstream infections across the UK and Ireland. Our results show that K. oxytoca has a highly diverse population, which is composed of several distinct clades, and we identified one recent expansion of a clone within our dataset. Although the K. oxytoca genomes are clearly distinct from the genomes of a global collection of Klebsiella pneumoniae complex, pre-dominantly composed of K. pneumoniae, we found evidence for sharing of core genes through recombination, as well as the exchange of accessory antimicrobial resistance and virulence factor genes between the species. Our findings also suggest that the different K. oxytoca clades have acquired antimicrobial resistance and virulence factor genes independently. This highlights the clinical and therapeutic importance of genetic flexibility in K. oxytoca and the relevance of this in its role as an opportunistic pathogen.

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“…2013; Chen et al. 2013), and one environmental isolate from South Korea (Shin et al. 2012), one isolate recovered from panda faeces in China (Jiang et al.…”

Section: Resultsmentioning

confidence: 99%

Population Structure of Multidrug-Resistant Klebsiella oxytoca within Hospitals across the United Kingdom and Ireland Identifies Sharing of Virulence and Resistance Genes with K. pneumoniae

Moradigaravand

Martin

Peacock

et al. 2017

Genome Biology and Evolution

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“…Some K. oxytoca strains are known due to their roles in biofuel production (e.g., K. oxytoca strain KCTC 1686 [4]) and biological nitrogen fixation (e.g., K. oxytoca strain M5a1 [5]).…”

Section: Genome Announcementmentioning

confidence: 99%

“…A draft genome containing 72 contigs was obtained based on the strain KCTC 1686 genome (accession no. CP003218) (4). …”

Section: Genome Announcementmentioning

confidence: 99%

Genome Sequence of Klebsiella oxytoca SA2, an Endophytic Nitrogen-Fixing Bacterium Isolated from the Pioneer Grass Psammochloa villosa

et al. 2013

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“…In this study, we cloned an amidase gene from K. oxytoca KCTC 1686 [29] and studied how to achieve efficient expression in E. coli. First, the amidase (KamH) was expressed as a fusion protein with a 6 kDa peptide containing a 6 × His-tag and an enterokinase cleavage site at its N-terminus; approximately half of the recombinant protein was expressed in soluble form, but it had no amidase activity.…”

Section: Introductionmentioning

confidence: 99%

Soluble and functional expression of a recombinant enantioselective amidase from Klebsiella oxytoca KCTC 1686 in Escherichia coli and its biochemical characterization

Guo

Yang

et al. 2015

Process Biochemistry

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Complete Genome Sequence of Klebsiella oxytoca KCTC 1686, Used in Production of 2,3-Butanediol

Abstract: eHere we report the full genome sequence of Klebsiella oxytoca KCTC 1686, which is used in production of 2,3-butanediol. The KCTC 1686 strain contains 5,974,109 bp with G؉C content of 56.05 mol% and contains 5,488 protein-coding genes and 110 structural RNAs.

Cited by 27 publications

References 12 publications

Population Structure of Multidrug-Resistant Klebsiella oxytoca within Hospitals across the United Kingdom and Ireland Identifies Sharing of Virulence and Resistance Genes with K. pneumoniae

Population Structure of Multidrug-Resistant Klebsiella oxytoca within Hospitals across the United Kingdom and Ireland Identifies Sharing of Virulence and Resistance Genes with K. pneumoniae

Genome Sequence of Klebsiella oxytoca SA2, an Endophytic Nitrogen-Fixing Bacterium Isolated from the Pioneer Grass Psammochloa villosa

Soluble and functional expression of a recombinant enantioselective amidase from Klebsiella oxytoca KCTC 1686 in Escherichia coli and its biochemical characterization

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