Complete Genome Sequence of Lactobacillus rhamnosus BFE5264, Isolated from Maasai Traditional Fermented Milk

Choi, Sang-Haeng; Ji, Yosep; Park, So‐Young; Mathara, Julius Maina; Holzapfel, Wilhelm H.; Kang, Jihee

doi:10.1128/genomea.00563-17

Cited by 1 publication

(1 citation statement)

References 6 publications

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“…To investigate whether our isolated strain is newly discovered, we compared the genomic information of PMC203 to other strains of L. rhamnosus such as BFE5264 31 , LRB 32 , BPL5 33 , DSM 14870 34 , and JL-1 35 (Supplementary Table S3 ). Although they belong to the same species, their source, genome size, G + C content, CDS (coding sequence), r-RNA, and t-RNA numbers differed.…”

Section: Resultsmentioning

confidence: 99%

In vitro anti-tuberculosis effect of probiotic Lacticaseibacillus rhamnosus PMC203 isolated from vaginal microbiota

Rahim

Seo

Kim

et al. 2022

Sci Rep

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Mycobacterium tuberculosis (M. tb), the etiological agent of tuberculosis (TB), poses a severe challenge for public health and remains the number one cause of death as a single infectious agent. There are 10 million active cases of TB per year with 1.5 million deaths, and 2–3 billion people are estimated to harbor latent M. tb infection. Moreover, the emergence of multi-drug-resistant (MDR), extremely-drug-resistant (XDR), and the recent totally drug-resistant (TDR) M. tb is becoming a global issue that has fueled the need to find new drugs different from existing regimens. In these circumstances, probiotics can be a potential choice, so we focused on developing them as an anti-tuberculosis drug candidate. Here, we report the anti-tubercular activities of Lacticaseibacillus rhamnosus PMC203 isolated from the vaginal microbiota of healthy women. PMC203 exhibited a promising intracellular killing effect against both drug-sensitive and resistant M. tb infected murine macrophage cell line RAW 264.7 without showing any cytotoxicity. Additionally, it also inhibited the growth of M. tb under broth culture medium. PMC203 did not cause weight change or specific clinical symptoms in a 2-week repeated oral administration toxicity test in a guinea pig model. Here, we also found that PMC203 induces autophagy in a dose dependent manner by increasing the signal of well-known autophagy gene markers, suggesting a possible intracellular killing mechanism.

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