Complete Genome Sequence of Lactococcus lactis subsp.
            <i>lactis</i>
            G121, an Isolate with Allergy-Protective Features Derived from a Farming Environment

Wang, Zhongjie; Minarsch, Eva-Maria L.; Kublik, Susanne; Heine, Holger; Schloter, Michael; Foesel, Bärbel U.

doi:10.1128/mra.00777-20

Cited by 1 publication

(1 citation statement)

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“…Based on comparative sequence analysis using BLAST, the conjugation‐associated gene cluster of pUC08B is genetically identical to that of pUC11B, while that of the recently discovered p001F plasmid (Wang et al, 2020 ) is almost identical to that to pUC11B and pUC08B. Furthermore, pC42 possesses three scattered conjugation‐related genes with similarity to those of the pUC11B system, however the limited number of genes that are present likely renders this system non‐functional.…”

Section: Resultsmentioning

confidence: 99%

Delineation of a lactococcal conjugation system reveals a restriction‐modification evasion system

Charneco

Kelleher

Buivydas

et al. 2023

Microbial Biotechnology

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Plasmid pUC11B is a 49.3-kb plasmid harboured by the fermented meat isolate Lactococcus lactis subsp. lactis UC11. Among other features, pUC11B encodes a pMRC01-like conjugation system and tetracycline-resistance. In this study, we demonstrate that this plasmid can be conjugated at high frequencies to recipient strains. Mutational analysis of the 22 genes encompassing the presumed pUC11B conjugation cluster revealed the presence of several genes with essential conjugation functions, as well as a gene, trsR, encoding a putative transcriptional repressor of this conjugation cluster. Furthermore, plasmid pUC11B encodes an anti-restriction protein, TrsAR, which facilitates higher conjugation frequencies when pUC11B is transferred into recipient strains containing Type II or Type III RM systems. These findings demonstrate how RM mechanisms can be circumvented when they act as a biological barrier for conjugation events.

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Section: Resultsmentioning

confidence: 99%