Complete Genome Sequence of Mycobacterium marinum ATCC 927
            <sup>T</sup>
            , Obtained Using Nanopore and Illumina Sequencing Technologies

Yoshida, Mitsunori; Fukano, Hanako; Miyamoto, Yuji; Shibayama, Keigo; Suzuki, Masato; Hoshino, Yoshihiko

doi:10.1128/genomea.00397-18

Cited by 8 publications

(10 citation statements)

References 21 publications

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“…Msp_012931 (GenBank AOPX01000000 ) is described in Kurokawa et al ( 50 ). Sources and GenBank numbers for other nodes are Mm_M, M. marinum M, CP000854 ( 22 ); Mm_MB2, M. marinum MB2, NZ_ANPM00000000 ( 51 ); Mm_Europe, M. marinum “Europe” ANPL00000000 ( 51 ); ATCC 927, M. marinum ATCC 927, NZ_AP018496 ( 41 ); E11, M. marinum E11, NZ_HG917972 ; Mps, M. pseudoshottsii , NZ_BCND01000000 ; Mul, M. ulcerans Agy99, CP000325 ( 16 ); Mlif, M. ulcerans ecovar Liflandii 128FXT, CP003899 ( 15 ). Other strains are as described in reference 14 .…”

Section: Resultsmentioning

confidence: 99%

“…PacBio sequencing of M. marinum ATCC 927 resulted in 137,098 reads, total read length of 1,210,762,761 bp, and ~144× coverage of two contigs of the chromosome (6,496,954 bp) and a plasmid (141,717 bp). This assembly of M. marinum ATCC 927 was used for analyses described in this work that occurred before the completed ATCC 927 genome was made available by a separate group ( NZ_AP018496 ) ( 41 ). Comparison of these assemblies indicated that they were syntenic and consistent in size, although the plasmid sequenced in the present work was longer than that reported in reference 41 (127,402 bp).…”

Section: Methodsmentioning

confidence: 99%

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Genomic Degeneration and Reduction in the Fish Pathogen Mycobacterium shottsii

et al. 2022

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Morone saxatilis (striped bass) is an ecologically and economically important finfish species on the United States east coast. Mycobacterium shottsii and Mycobacterium pseudoshottsii were originally described in the early 2000s as novel species from outbreaks of visceral and dermal mycobacteriosis in this species. Biochemical and genetic characterization place these species within the Mycobacterium ulcerans / M. marinum clade (MuMC), and M. pseudoshottsii has been proposed as an ecovar of M. ulcerans .

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genomic Degeneration and Reduction in the Fish Pathogen Mycobacterium shottsii

et al. 2022

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“…MMNAT was expressed in E. coli BL21(DE3)pLysS cells transformed with the pET28b(+) vector containing the nat gene (MMAR_5055) insert and purified using nickel (Ni 2+ ) ion affinity chromatography, as described previously [ 13 , 28 ]. Ten microliters of MMNAT enzyme (0.1 µg/µL), i.e., 1 µg of the enzyme in each well, was incubated with 45 µL of hydralazine (Sigma Aldrich, London, UK) (final concentration: 450 µM) for 5 min at RT.…”

Section: Methodsmentioning

confidence: 99%

3-(5-Nitrofuran-2-yl)prop-2-en-1-one Derivatives, with Potent Antituberculosis Activity, Inhibit A Novel Therapeutic Target, Arylamine N-acetyltransferase, in Mycobacteria

et al. 2020

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In this study, the inhibitory potential of 3-(5-nitrofuran-2-yl)prop-2-en-1-one derivatives was evaluated against a panel of bacteria, as well as mammalian cell lines to determine their therapeutic index. In addition, we investigated the mechanism of antibiotic action of the derivatives to identify their therapeutic target. We discovered compound 2 to be an extremely potent inhibitor of Mycobacterium tuberculosis H37Rv growth (MIC: 0.031 mg/L) in vitro, performing better than the currently used first-line antituberculosis drugs such as isoniazid, rifampicin, ethambutol, and pretomanid in vitro. Furthermore, compound 3 was equipotent to pretomanid against a multidrug-resistant M. tuberculosis clinical isolate. The derivatives were selective and bactericidal towards slow-growing mycobacteria. They showed low cytotoxicity towards murine RAW 264.7 and human THP-1 cell lines, with high selectivity indices. Compound 1 effectively eliminated the intracellular mycobacteria in a mycobacteria-infected macrophage model. The derivatives were assessed for their potential to inhibit mycobacterial arylamine N-acetyltransferase (NAT) and were identified as good inhibitors of recombinant mycobacterial NAT, a novel target essential for the intracellular survival of M. tuberculosis. This study provided hits for designing new potent and selective antituberculosis leads, having mycobacterial NAT inhibition as their possible endogenous mechanisms of action.

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“…Alignments produced by mapping Illumina reads to the assembly using the Burrows–Wheeler aligner ( 13 ) were used for correction of sequence and assembly errors with Pilon ( 14 ). Automated annotation was carried out with the DDBJ Fast Annotation and Submission Tool (DFAST) ( https://dfast.nig.ac.jp ) ( 8 , 9 , 15 , 16 ). Average nucleotide identity (ANI) was calculated by JSpeciesWS ( 17 ).…”

Section: Genome Announcementmentioning

confidence: 99%