Complete Genome Sequence of Mycoplasma flocculare Strain Ms42
            <sup>T</sup>
            (ATCC 27399
            <sup>T</sup>
            )

Calcutt, Michael J.; Foecking, Mark F.; Heidari, M. B.; McIntosh, Mark A.

doi:10.1128/genomea.00124-15

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Mycoplasma flocculare was first identified in 1972 and was generally considered to be a commensal colonizer of the porcine nasopharynx but an opportunist pulmonary pathogen in coinfections with Mycoplasma hyopneumoniae (Meyling and Friis, ). Recently, complete genome sequence of Mycoplasma flocculare strain Ms42 T was reported, which would enable more detailed analyses of genome structure and plasticity among the Mycoplasma species (Calcutt et al ., ). In this study, both Mycoplasma hyopneumoniae and Mycoplasma flocculare showed strong association with lung lesions.…”

Section: Discussionmentioning

confidence: 97%

Microbial communities in swine lungs and their association with lung lesions

Huang

Zhang

Tong

et al. 2018

Microbial Biotechnology

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Summary Under natural farming, environmental pathogenic microorganisms may invade and affect swine lungs, further resulting in lung lesions. However, few studies on swine lung microbiota and their potential relationship with lung lesions were reported. Here, we sampled 20 pigs from a hybrid herd raised under natural conditions; we recorded a lung‐lesion phenotype and investigated lung microbial communities by sequencing the V3‐V4 region of 16S rRNA gene for each individual. We found reduced microbial diversity but more biomass in the severe‐lesion lungs. Methylotenera , Prevotella , Sphingobium and Lactobacillus were the prominent bacteria in the healthy lungs, while Mycoplasma , Ureaplasma , Sphingobium , Haemophilus and Phyllobacterium were the most abundant microbes in the severe‐lesion lungs. Notably, we identified 64 lung‐lesion‐associated OTU s, of which two classified to Mycoplasma were positively associated with lung lesions and 62 showed negative association including thirteen classified to Prevotella and six to Ruminococcus . Cross‐validation analysis showed that lung microbiota explained 23.7% phenotypic variance of lung lesions, suggesting that lung microbiota had large effects on promoting lung healthy. Furthermore, 22 KEGG pathways correlated with lung lesions were predicted. Altogether, our findings improve the knowledge about swine lung microbial communities and give insights into the relationship between lung microbiota and lung lesions.

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Section: Discussionmentioning

confidence: 97%

Microbial communities in swine lungs and their association with lung lesions

Huang

Zhang

Tong

et al. 2018

Microbial Biotechnology

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“…Our results showed that all evaluated strains initiate the production of all tested cytokines, which indicates an interaction between mycoplasmal components and BM-DCs receptors. This type of interaction has never been described for M. flocculare , which is considered as being, according to different authors, either a commensal of the respiratory tract or a potential PRDC-associated agent [1,25].…”

Section: Discussionmentioning

confidence: 99%

Intra-Species and Inter-Species Differences in Cytokine Production by Porcine Antigen-Presenting Cells Stimulated by Mycoplasma hyopneumoniae, M. hyorhinis, and M. flocculare

et al. 2019

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Mycoplasma hyorhinis and M. flocculare are commonly co-isolated with M. hyopneumoniae (primary agent of swine enzootic pneumonia) in gross pneumonia-like lesions, but their involvement in the disease process remains unknown. T cells play an immuno-pathological role during mycoplasmal infections. Dendritic cells (DCs) are major antigen-presenting cells involved in T cell activation and differentiation. In this study, we investigated cytokine (IL-6, IL-8, IL-10, IL-12, and TNF-α) production by porcine bone-marrow-derived DCs (BM-DCs) stimulated by M. hyopneumoniae, M. hyorhinis, and/or M. flocculare. Results showed that cytokine production levels were relatively homogenous for all evaluated M. hyopneumoniae strains in contrast to M. hyorhinis and M. flocculare strains. The most noteworthy inter-species differences were the overall (i) lower IL-12 production capacity of M. hyopneumoniae, and (ii) higher TNF-α production capacity of M. flocculare. Co-stimulation of BM-DCs showed that M. hyorhinis dominated the IL-12 production independently of its association with M. hyopneumoniae or M. flocculare. In addition, a decreased BM-DC production of TNF-α was generally observed in the presence of mycoplasma associations. Lastly, M. flocculare association with M. hyopneumoniae increased BM-DC ability to secrete IL-10. A higher cytotoxicity level in BM-DCs stimulated by M. hyorhinis was also observed. Overall, this study demonstrated that the combination of M. hyorhinis or M. flocculare with M. hyopneumoniae may participate to the modulation of the immune response that might affect the final disease outcome.

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“…Mfloc and Mhr are etiologically associated with Mhyo (Siqueira et al, 2016). Mfloc was considered a pathogen associated with PRDC, potentially opportunistic in the case of co-infection with Mhyo (Calcutt et al, 2015). However, the others authors related that Mfloc is a commensal microorganism of the pig respiratory tract (Friis, 1974;Strasser et al, 1992;Martello et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

Ferreira

Mechler-Dreibi

Sonálio

et al. 2021

Veterinary Microbiology

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Complete Genome Sequence of Mycoplasma flocculare Strain Ms42 ^T (ATCC 27399 ^T )

Cited by 5 publications

References 12 publications

Microbial communities in swine lungs and their association with lung lesions

Microbial communities in swine lungs and their association with lung lesions

Intra-Species and Inter-Species Differences in Cytokine Production by Porcine Antigen-Presenting Cells Stimulated by Mycoplasma hyopneumoniae, M. hyorhinis, and M. flocculare

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

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Complete Genome Sequence of Mycoplasma flocculare Strain Ms42 T (ATCC 27399 T )

Cited by 5 publications

References 12 publications

Microbial communities in swine lungs and their association with lung lesions

Microbial communities in swine lungs and their association with lung lesions

Intra-Species and Inter-Species Differences in Cytokine Production by Porcine Antigen-Presenting Cells Stimulated by Mycoplasma hyopneumoniae, M. hyorhinis, and M. flocculare

Co-infections by Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare in macroscopic lesions of lung consolidation of pigs at slaughter

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Complete Genome Sequence of Mycoplasma flocculare Strain Ms42 ^T (ATCC 27399 ^T )