Complete Genome Sequence of NAH7-Harboring Pseudomonas putida Strain G7

Varner, Paige M.; Gunsch, Claudia K.

doi:10.1128/mra.00394-22

Cited by 2 publications

(1 citation statement)

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“…Evaluation of siRNA Impacts on Bacterial Growth in Microenvironments. To study the changes in growth patterns and viability resulting from siRNA exposure, model bacteria Bacillus subtilis (GC: 43.6%), 29 Pseudomonas putida G7 (GC: 62.13%), 30 and Paenibacillus sp. 300A (GC: 40.5%) 31 were cultured in R2A media with and without siRNAs and additional external stresses.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Small Interfering RNAs (siRNAs) Negatively Impact Growth and Gene Expression of Environmentally Relevant Bacteria in In Vitro Conditions

Un Jan Contreras,

Redfern,

Maguire

et al. 2024

Environ. Sci. Technol.

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Rising global populations have amplified food scarcity and ushered in the development of genetically modified (GM) crops containing small interference RNAs (siRNAs) that control gene expression to overcome these challenges. The use of RNA interference (RNAi) in agriculture remains controversial due to uncertainty regarding the unintended release of genetic material and downstream nontarget effects, which have not been assessed in environmental bacteria to date. To evaluate the impacts of siRNAs used in agriculture on environmental bacteria, this study assessed microbial growth and viability as well as transcription activity with and without the presence of environmental stressors. Results showed a statistically significant reduction in growth capacity and maximum biomass achieved when bacteria are exposed to siRNAs alone and with additional external stress (p < 0.05). Further transcriptomic analysis demonstrated that nutrient cycling gene activities were found to be consistently and significantly altered following siRNA exposure, particularly among carbon (xylA, FBPase, limEH, Chitinase, rgl, rgh, rgaE, mannanase, ara) and nitrogen (ureC, nasA, narB, narG, nirK) cycling genes (p < 0.05). Decreases in carbon cycling gene transcription profiles were generally significantly enhanced when siRNA exposure was coupled with nutrient or antimicrobial stress. Collectively, findings suggest that certain conditions facilitate the uptake of siRNAs from their surrounding environments that can negatively affect bacterial growth and gene expression activity, with uncertain downstream impacts on ecosystem homeostasis.

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Section: ■ Materials and Methodsmentioning

confidence: 99%