Complete Genome Sequence of Novel Porcine Epidemic Diarrhea Virus Strain GD-1 in China

wei, Zhong Yan; Lu, Wen Hui; Li, Zhi Li; Mo, Jian; Zeng, Xi Duo; Zeng, Zhi Liang; Sun, Bao Li; Chen, Feng; Xie, Qing; Bee, Ying Zuo; Yun, Jing

doi:10.1128/jvi.02615-12

Cited by 16 publications

(12 citation statements)

References 10 publications

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“…Subgroup Gd strains were first identified in Guangdong Province during 2011 to 2013 and then rapidly spread to nearby areas (Chen et al., ; Fan et al., ; Wei et al., ; Tian et al., ). The CH‐SDLY‐2‐2014 and CH‐SDLY‐3‐2014 strains were first identified from Linyi, Shandong Province in 2014, which were further verified to be subgenotype Gd.…”

Section: Discussionmentioning

confidence: 99%

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Zhang

Tian

Lin

et al. 2017

Transbound Emerg Dis

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As one of the top pork producers in China, Shandong Province suffered frequent outbreaks of porcine epidemic diarrhoea virus (PEDV) on pig farms from January 2012 to July 2015, resulting in significant economic losses. To better understand the prevalence situation, we conducted molecular epidemiological analyses of 38 PEDV strains isolated from 13 cities in Shandong Province. The detection rate of PEDV was 71.2% (146/205) by reverse transcription polymerase chain reaction (RT-PCR). The S genes of the 38 isolated samples were 4146 to 4161 nt in length and shared high levels of sequence identity (93.3-99.6% nt, 92.1-99.4% aa) with those of the 41 reference strains. Among the 38 strains, 31 strains that occupied 12 cities were classed into G3 genotype, while the other seven that only existed in four cities were classed into G2 genotype. In addition, the strains CH-SDLY-2-2014 and CH-SDLY-3-2014 isolated from Linyi were classed into the Gd subgenotype. Notably, there were multiple insertions or deletions in the S genes and several mutations in the neutralizing epitopes of the PEDV S protein. Overall, the results revealed that G2 and G3 are the predominant PEDV genotypes circulating in Shandong Province during 2012-2015, and Gd subgenotype in G3 group had already spread towards northern China in 2014.

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Section: Discussionmentioning

confidence: 99%

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Zhang

Tian

Lin

et al. 2017

Transbound Emerg Dis

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“…As PEDV is highly contagious, the resulting economic burden to the swine industry worldwide is substantial (Bertasio et al, 2016;Gerber et al, 2016;Wang et al, 2014a). Since December 2010, swine farms in China have seen large-scale outbreaks of PEDV infection with typical mortality rates of 50-90% in suckling piglets Chen et al, 2012;Wei et al, 2012). In the United States, PEDV pandemic strains have caused more than 80 % mortality in suckling piglets and have been rapidly transmitted between farms since 2013.…”

Section: Introductionmentioning

confidence: 99%

Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis

Lin

Liu

et al. 2020

Veterinary Microbiology

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Porcine epidemic diarrhea virus (PEDV) has caused, and continues to cause, severe economic losses to the swine industry worldwide. The pathogenic mechanism and immune regulatory interactions between PEDV and the host remain largely unknown. In this study, the interaction between autophagy and PEDV replication in intestinal porcine epithelial (IPEC-J2) cells was investigated. The effects of the structural and nonstructural proteins of PEDV on the autophagy process and the autophagy-related signaling pathways were also examined. The results shown that PEDV replication increased the autophagy flux in IPEC-J2 cells, and that autophagy was beneficial to PEDV replication, which may be one of the reasons for the rapid damage to intestinal epithelial cells and the enhanced virulence of PEDV in both newborn piglets and finishing pigs. When autophagy was pharmacologically induced by rapamycin, PEDV replication increased from 8.5 × 10 5 TCID 50 /mL to 8.8 × 10 6 TCID 50 /mL in IPEC-J2 cells. When autophagy was pharmacologically suppressed by hydroxychloroquine, PEDV replication decreased from 8.5 × 10 5 TCID 50 /mL to 7.9 × 10 4 TCID 50 /mL. To identify which PEDV proteins were the key inducers of autophagy, all 4 structural proteins and 17 nonstructural proteins of PEDV were eukaryotic expressed. It was found that the nonstructural protein 6 (nsp6) and ORF3 of PEDV were able to induce significant autophagy in IPEC-J2 cells, but the other proteins were unable to induce autophagy. It was indicated that nsp6-induced autophagy mainly occurred via the PI3K/Akt/mTOR signaling pathway. The results accelerate the understanding of the biology and pathogenesis of PEDV infection and provide new insights into the development of effective therapeutic strategies.

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“…Because of incomplete genomic information, most molecular epidemiological analyses have targeted a single gene of PEDV. To date, complete genome sequences of 18 PEDV strains, including 10 endemic Chinese isolates, have been submitted to the GenBank database [4,[8][9][10][11][12][13][14][15][16][17][18][19]. To better understand the diversity, epidemiology and molecular characteristics of PEDV isolates in China, we sequenced the complete genome of a virulent PEDV strain from Henan Province, China.…”

Section: Introductionmentioning

confidence: 99%

Genetic properties of endemic Chinese porcine epidemic diarrhea virus strains isolated since 2010

Wang

Niu

et al. 2013

Arch Virol

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Acute diarrhea outbreaks caused by porcine epidemic diarrhea virus (PEDV) have been observed in various pig-breeding provinces of China since December 2010. Endemic strains of PEDV were isolated from different areas, and the complete genome sequences of 10 isolates were determined. Our objective in this study was to genetically characterize current Chinese field isolates of PEDV to better understand their epidemiology and genetic diversity. Sequence analysis showed that 10 post-2010 isolates shared high homology with each other and were always clustered together with the virulent DR13 strains (South Korea) and/or one earlier Chinese strain, CH-S, in phylogenetic analysis. All post-2010 isolates possessed common sequence changes in each gene. Our results suggest that current Chinese PEDV isolates originated from either South Korean and/or Chinese ancestors that underwent some genetic variation, thereby forming a new PEDV genotype in China.

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Complete Genome Sequence of Novel Porcine Epidemic Diarrhea Virus Strain GD-1 in China

Cited by 16 publications

References 10 publications

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Nonstructural protein 6 of porcine epidemic diarrhea virus induces autophagy to promote viral replication via the PI3K/Akt/mTOR axis

Genetic properties of endemic Chinese porcine epidemic diarrhea virus strains isolated since 2010

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