Complete genome sequence of novel isolate SYJ15 of Bacillus cereus group, a highly lethal pathogen isolated from Chinese soft shell turtle (Pelodiscus Sinensis)

Yuan, Xuemei; Lyu, Sunjian; Zhang, Haiqi; Hang, Xiaoying; Shi, Weida; Liu, Li; Yinglei, Wu

doi:10.1007/s00203-019-01723-y

Cited by 12 publications

(8 citation statements)

References 17 publications

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“…The main genotype groups QF108045, QF108088, QF107009, QF107010, QF106004, QF105006 and QF105032 (N4, S4 and E4) belonged to Group 1 in both 16S rDNA and pycA , they were closely related to one Bacillus sp. strain (accession number: CP036356.1) that was isolated from diseased CST in China, showed 99.5% and 100% similarity, respectively (Yuan et al., 2020) (Figures 6 and 7).…”

Section: Resultsmentioning

confidence: 99%

“…Diseases caused by viral and bacterial infections remain a major threat to the CST culture industry in commercial culture systems (Chen et al, 1999). In the CST, Bacillus cereus group (Bcg) infection characterized by gross lesions such as hepatic congestion and swollen spleen causes high mortality, resulting in huge economic losses and establishing them as one of the most prevalent pathogenic microorganisms (Tan et al, 2011;Yuan et al, 2020). The Bcg includes several closely related species, including B. cereus (Bc), B. thuringiensis (Bt), B. anthracis, B. mycoides, B. pseudomycoides, B. weihenstephanensis and B. cytotoxicus (Guinebretière et al, 2013;Zwick et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The pycA sequences of the clade 1 sequencing strains (N4, S4 and E4) were 99.8% identical to clade 1 Bt strain (JQ728640.1) and 98.8% identical to clade 1 Bc E4) belonged to Group 1 in both 16S rDNA and pycA, and they were closely related to one Bacillus sp. strain (accession number: CP036356.1) that was isolated from diseased CST in China and showed 99.5% and 100% similarity, respectively(Yuan et al, 2020) (Figures6 and 7).…”

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confidence: 99%

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Genotype and virulence gene analyses of Bacillus cereus group clinical isolates from the Chinese softshell turtle (Pelodiscus sinensis) in Taiwan

Cheng

Rao

Poudyal

et al. 2021

Journal of Fish Diseases

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Chinese softshell turtles (Pelodiscus sinensis) (CST) are susceptible to infections by bacteria belonging to the Bacillus cereus group (Bcg). Bcg includes several closely related species, two of which, B. cereus and B. thuringiensis, are pathogens of aquatic animals or insects. In the present study, we collected 57 Bcg isolates obtained from diseased CST from 2016 to 2019 in Kaohsiung and Pingtung, the areas with the most CST farms in Taiwan. All isolates were divided into four genotypes with two restriction enzymes, SmaI and NotI, by pulsed-field gel electrophoresis and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR).Representative isolates from each genotype were subjected to phylogenetic tree analysis using 16S rDNA and pyruvate carboxylase genes as phylogenetic markers, and these CST isolates appeared in different clades. PCR was performed targeting six selected virulence genes, four of which were detected in CST isolates, including cytotoxin K (1/57), hblC of the haemolysin BL complex (46/57), nheA of the nonhaemolytic enterotoxin complex (52/57) and enterotoxin FM (57/57), whereas cereulide synthetase and cereulide peptide synthase-like genes were not detected in any isolates.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Genotype and virulence gene analyses of Bacillus cereus group clinical isolates from the Chinese softshell turtle (Pelodiscus sinensis) in Taiwan

Cheng

Rao

Poudyal

et al. 2021

Journal of Fish Diseases

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“…Some bacterial toxic proteins are entomopathogenic, showing specificity to target species. Bacillus thuringiensis is a Gram positive, spore-forming bacterium that synthesizes parasporal crystalline inclusions containing Cry and Cyt proteins that have been successfully used as bioinsecticides (Yuan et al, 2019). It expresses the crystal protein Cry that promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects.…”

Section: Proteins Causing Toxic Effects Per Sementioning

confidence: 99%

Literature search – Exploring in silico protein toxicity prediction methods to support the food and feed risk assessment

Palazzolo

Gianazza

Eberini

2020

EFSA Supporting Publications

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This report is the outcome of an EFSA procurement (NP/EFSA/GMO/2018/01) reviewing relevant scientific information on in silico prediction methods for protein toxicity, that could support the food and feed risk assessment. Several proteins are associated with adverse (toxic) effects in humans and animals, by a variety of mechanisms. These are produced by plants, animals and bacteria to prevail in hostile environments. In the present report, we present an integrated pipeline to perform a comprehensive literature and database search applied to proteins with toxic effects. "Toxin activity" and "toxin-antitoxin system" strings were used as inputs for this pipeline. UniProtKB was considered as the reference database, and only the UniProtKB curator-reviewed proteins were considered in the pipeline. Experimentallydetermined structures and homology-based in silico 3D models were retrieved from protein structures repositories; family-, domain-, motif-and other molecular signature-related information was also obtained from specific databases which are part of the InterPro consortium. Protein aggregation associated with adverse effects was also investigated using different search strategies. This work can serve as the basis for further exploring novel risk assessment strategies for new proteins using in silico predictive methods.

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“…The Chinese soft-shell turtle (Pelodiscus sinensis), belongs to the Reptilia, Testudines and turtle family, and is important in Asia (Yang et al, 2011). Chinese soft-shell turtle farming is associated with many infectious diseases during superintensive aquaculture growth (Zhou et al, 2008), including viruses, bacteria (Yuan et al, 2020) and fungi (Takuma et al, 2011), causing severe losses in this industry.…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft‐shell turtles

Chen,

Xue,

Chen

et al. 2024

Journal of Fish Diseases

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Aeromonas jandaei is a gram‐negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft‐shell turtle (Pelodiscus sinensis), then named strain JDP‐FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical characteristics, as well as 16S rRNA and gyrB gene sequences. Virulence genetic testing further detected temperature‐sensitive protease (eprCAI), type III secretion system (TTSS) (ascv), nuclease (nuc), cytotonic enterotoxin (alt) and serine proteinase (ser) in JDP‐FX. Compared with healthy Chinese soft‐shell turtle, the serum levels of total protein (TP), albumin (ALB) and globulin (GLB) were significantly decreased in the diseased Chinese soft‐shell turtle, while, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased. Histopathological observations showed that multiple tissues, including intestinal mucosa, liver and kidney, were severely damaged in the diseased Chinese soft‐shell turtle. Moreover, the diseased Chinese soft‐shell turtle had significant cell degeneration, necrosis, sloughing and interstitial inflammatory cell infiltration. The pathogenicity of JDP‐FX was tested via artificial infection. The median lethal dosage (LD50) of the strain was 1.05 × 105 colony forming units (CFU/g) per weight of Chinese soft‐shell turtle. Drug susceptibility analysis revealed that JDP‐FX was susceptible to ceftazidime, minocycline, cefoperazone, ceftriaxone and piperacillin. In addition, JDP‐FX was resistant to doxycycline, florfenicol, sulfonamides, gentamicin, ampicillin and neomycin. Therefore, this study may provide guidance for further research into the diagnosis, prevention and treatment of JDP‐FX infection.

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Complete genome sequence of novel isolate SYJ15 of Bacillus cereus group, a highly lethal pathogen isolated from Chinese soft shell turtle (Pelodiscus Sinensis)

Cited by 12 publications

References 17 publications

Genotype and virulence gene analyses of Bacillus cereus group clinical isolates from the Chinese softshell turtle (Pelodiscus sinensis) in Taiwan

Genotype and virulence gene analyses of Bacillus cereus group clinical isolates from the Chinese softshell turtle (Pelodiscus sinensis) in Taiwan

Literature search – Exploring in silico protein toxicity prediction methods to support the food and feed risk assessment

Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft‐shell turtles

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