Complete Genome Sequence of Pronghorn Virus, a Pestivirus

Neill, John D.; Ridpath, Julia F.; Fischer, Nicole; Grundhoff, Adam; Postel, Alexander; Becher, Paul

doi:10.1128/genomea.00575-14

Cited by 14 publications

(9 citation statements)

References 7 publications

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“…The diluted library (2 nM) was sequenced on an Illumina MiSeq instrument (2x250bp paired end; 4.078.934 reads). De novo assembly and taxonomic classification of the assembled contig were performed as described (Becher et al, 2014;Neill et al, 2014).…”

Section: Complete Genome Sequence Of the Csfv Pinar Del Rio Field Strainmentioning

confidence: 99%

Novel poly-uridine insertion in the 3′UTR and E2 amino acid substitutions in a low virulent classical swine fever virus

Coronado

Liniger²,

Muñoz-González

et al. 2017

Veterinary Microbiology

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In this study, we compared the virulence in weaner pigs of the Pinar del Rio isolate and the virulent Margarita strain. The latter caused the Cuban classical swine fever (CSF) outbreak of 1993. Our results showed that the Pinar del Rio virus isolated during an endemic phase is clearly of low virulence. We analysed the complete nucleotide sequence of the Pinar del Rio virus isolated after persistence in newborn piglets, as well as the genome sequence of the inoculum. The consensus genome sequence of the Pinar del Rio virus remained completely unchanged after 28days of persistent infection in swine. More importantly, a unique poly-uridine tract was discovered in the 3'UTR of the Pinar del Rio virus, which was not found in the Margarita virus or any other known CSFV sequences. Based on RNA secondary structure prediction, the poly-uridine tract results in a long single-stranded intervening sequence (SS) between the stem-loops I and II of the 3'UTR, without major changes in the stem- loop structures when compared to the Margarita virus. The possible implications of this novel insertion on persistence and attenuation remain to be investigated. In addition, comparison of the amino acid sequence of the viral proteins E, E1, E2 and p7 of the Margarita and Pinar del Rio viruses showed that all non-conservative amino acid substitutions acquired by the Pinar del Rio isolate clustered in E2, with two of them being located within the B/C domain. Immunisation and cross-neutralisation experiments in pigs and rabbits suggest differences between these two viruses, which may be attributable to the amino acid differences observed in E2. Altogether, these data provide fresh insights into viral molecular features which might be associated with the attenuation and adaptation of CSFV for persistence in the field.

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Section: Complete Genome Sequence Of the Csfv Pinar Del Rio Field Strainmentioning

confidence: 99%

Novel poly-uridine insertion in the 3′UTR and E2 amino acid substitutions in a low virulent classical swine fever virus

Coronado

Liniger²,

Muñoz-González

et al. 2017

Veterinary Microbiology

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“…Considering all pestivirus genus, Pronghorn, Bungowannah and Atypical Putative Porcine Virus (App V), are more divergent to those virus than Hobi-like (BAUERMANN et al, 2012;NEILL et al, 2014;HAUSE et al, 2015;. However, we cannot rule out that the neutralizing antibodies detected were induced by other pestivirus infection than BVDV-1.…”

Section: Resultsmentioning

confidence: 81%

A cross-sectional study to estimate the frequency of anti-bovine viral diarrhea virus-1 antibodies in domestic pigs of Mossoró region in the state of Rio Grande do Norte, Brazil

et al. 2016

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“…Until 2017, only four "classical" species belonged to the genus Pestivirus: bovine viral diarrhea virus 1 (BVDV-1); bovine viral diarrhea virus 2 (BVDV-2); border disease virus (BDV); and CSFV [22]. However, a large number of viruses linked to pestiviruses have been described [1,[23][24][25][26][27][28][29][30][31][32], and Smith et al [22] proposed that the study group of the Flaviviridae family of the International Committee on Viral Taxonomy (ICTV) revise the taxonomy of the genus Pestivirus. Thus, the genus Pestivirus was divided into 11 species, Pestivirus A (BVDV-1) [33], Pestivirus B (BVDV-2) [34], Pestivirus C (CSFV) [35], Pestivirus D (BDV) [36], Pestivirus E (pronghorn antelope pestivirus) [29], Pestivirus F (porcine pestivirus) [26], Pestivirus G (giraffe pestivirus) [23], Pestivirus H (HoBi-like pestivirus) [27], Pestivirus I (Aydin-like pestivirus) [24], Pestivirus J (rat pestivirus) [25], and Pestivirus K (atypical porcine pestivirus) [1], and one unclassified virus, bat pestivirus [30].…”

Section: Classification and Etiologymentioning

confidence: 99%

Pestivirus K (Atypical Porcine Pestivirus): Update on the Virus, Viral Infection, and the Association with Congenital Tremor in Newborn Piglets

Agnol

Alfieri

2020

Viruses

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The atypical porcine pestivirus (APPV) belongs to the species Pestivirus K of the genus Pestivirus and the family Flaviviridae, and it has been associated with congenital tremor (CT) type A-II in newborn piglets. Although APPV was discovered in 2015, evidence shows that APPV has circulated in pig herds for many years, at least since 1986. Due to the frequently reported outbreaks of CT on different continents, the importance of this virus for global pig production is notable. Since 2015, several studies have been conducted to clarify the association between APPV and CT. However, some findings regarding APPV infection and the measures taken to control and prevent the spread of this virus need to be contextualized to understand the infection better. This review attempts to highlight advances in the understanding of APPV associated with type A-II CT, such as etiology, epidemiology, diagnosis, and control and prevention measures, and also describes the pathophysiology of the infection and its consequences for pig production. Further research still needs to be conducted to elucidate the host’s immune response to APPV infection, the control and prevention of this infection, and the possible development of vaccines.

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Complete Genome Sequence of Pronghorn Virus, a Pestivirus

Cited by 14 publications

References 7 publications

Novel poly-uridine insertion in the 3′UTR and E2 amino acid substitutions in a low virulent classical swine fever virus

Novel poly-uridine insertion in the 3′UTR and E2 amino acid substitutions in a low virulent classical swine fever virus

A cross-sectional study to estimate the frequency of anti-bovine viral diarrhea virus-1 antibodies in domestic pigs of Mossoró region in the state of Rio Grande do Norte, Brazil

Pestivirus K (Atypical Porcine Pestivirus): Update on the Virus, Viral Infection, and the Association with Congenital Tremor in Newborn Piglets

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