Complete Genome Sequence of Pseudomonas otitidis Strain MrB4, Isolated from Lake Biwa in Japan

Miyazaki, Kentaro; Hase, Erina; Maruya, Tomomasa

doi:10.1128/mra.00148-20

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…In 2021, the first case of bacteremia was reported [ 4 ]. Previous reports indicate its environmental distribution in Nigeria and Lake Kawaguchi in Japan [ 10 , 11 ], and concerns have been raised regarding its potential to cause infections in immunocompromised patients and more severe presentations [ 2 ]. To the best of our knowledge, this is the first case report of bacteremia caused by P. otitidis in an immunocompromised patient, highlighting the potential risks of severe infections in such patients.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas otitidis bacteremia in an immunocompromised patient with cellulitis: case report and literature review

Mori,

Yoshizawa,

Yamada

et al. 2023

BMC Infect Dis

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Background Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidis has a unique susceptibility profile, being susceptible to penicillins and cephalosporins but resistant to carbapenems, due to the production of the metallo-β-lactamase called POM-1. This revealed genetic similarities with Pseudomonas aeruginosa, which can sometimes lead to misidentification. Case presentation We report the case of a 70-year-old Japanese male who developed cellulitis and bacteremia during chemotherapy for multiple myeloma. He was initially treated with meropenem, but blood culture later revealed gram-negative bacilli identified as P. otitidis using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Carbapenem resistance was predicted from previous reports; therefore, we switched to dual therapy with levofloxacin and cefepime, and favorable treatment results were obtained. Conclusion This is the first reported case of P. otitidis cellulitis and bacteremia in an immunocompromised patient. Carbapenems are typically used in immunocompromised patients and P. otitidis is often resistant to it. However, its biochemical properties are similar to those of Pseudomonas aeruginosa; therefore, its accurate identification is critical. In the present study, we rapidly identified P. otitidis using MALDI-TOF MS and switched from carbapenems to an appropriate antimicrobial therapy, resulting in a successful outcome.

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Section: Discussionmentioning

confidence: 99%

Pseudomonas otitidis bacteremia in an immunocompromised patient with cellulitis: case report and literature review

Mori,

Yoshizawa,

Yamada

et al. 2023

BMC Infect Dis

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“…Subgroup II strains, isolated from infected blood (MRY13-0052) and minced oyster tissue (OT69), clustered together with P. otitidis strain MrB4. Although P. otitidis was initially isolated from a patient with an ear infection, this species is also widespread in nonclinical environments, as was MrB4, isolated from the near-shore area of Lake Biwa in Japan [ 73 ]. In agreement with these results, a recent study showed that a pair-wise comparison of P. alcaligenes OT69 against several type-strain genomes indicated P. otitidis DSM 17224 as its closest relative, rather than the type P. alcaligenes strain NBRC 14159, suggesting that OT69 should be included in a potentially new species [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

The Rhizobacterium Pseudomonas alcaligenes AVO110 Induces the Expression of Biofilm-Related Genes in Response to Rosellinia necatrix Exudates

et al. 2021

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The rhizobacterium Pseudomonas alcaligenes AVO110 exhibits antagonism toward the phytopathogenic fungus Rosellinia necatrix. This strain efficiently colonizes R. necatrix hyphae and is able to feed on their exudates. Here, we report the complete genome sequence of P. alcaligenes AVO110. The phylogeny of all available P. alcaligenes genomes separates environmental isolates, including AVO110, from those obtained from infected human blood and oyster tissues, which cluster together with Pseudomonas otitidis. Core and pan-genome analyses showed that P. alcaligenes strains encode highly heterogenic gene pools, with the AVO110 genome encoding the largest and most exclusive variable region (~1.6 Mb, 1795 genes). The AVO110 singletons include a wide repertoire of genes related to biofilm formation, several of which are transcriptionally modulated by R. necatrix exudates. One of these genes (cmpA) encodes a GGDEF/EAL domain protein specific to Pseudomonas spp. strains isolated primarily from the rhizosphere of diverse plants, but also from soil and water samples. We also show that CmpA has a role in biofilm formation and that the integrity of its EAL domain is involved in this function. This study contributes to a better understanding of the niche-specific adaptations and lifestyles of P. alcaligenes, including the mycophagous behavior of strain AVO110.

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“…In 2021, the rst case of bacteremia was reported [4]. Previous reports indicate its environmental distribution in Nigeria and Lake Kawaguchi in Japan [9,10], and concerns have been raised regarding its potential to cause infections in immunocompromised patients and more severe presentations [2]. To the best of our knowledge, this is the rst case report of severe infection caused by P. otitidis in an immunocompromised patient, highlighting the potential risks of severe infections in such patients.…”

Section: Case Presentationmentioning

confidence: 91%

Pseudomonas otitidis bacteremia in an immunocompromised patient with cellulitis: case report and literature review

Mori,

Yoshizawa,

Yamada

et al. 2023

Preprint

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Background: Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidishas a unique susceptibility profile, being susceptible to penicillins and cephalosporins but resistant to carbapenems, due to the production of the metallo-β-lactamase called POM-1. This revealed genetic similarities with Pseudomonas aeruginosa, which can sometimes lead to misidentification. Case presentation: We report the case of a 70-year-old Japanese male who developed cellulitis and bacteremia during chemotherapy for multiple myeloma. He was initially treated with meropenem, but blood culture later revealed gram-negative bacilli identified as P. otitidis using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Carbapenem resistance was predicted from previous reports; therefore, we switched to dual therapy with levofloxacin and cefepime, and favorable treatment results were obtained. Conclusion: This is the first reported case of P. otitidis cellulitis and bacteremia in an immunocompromised patient. Carbapenems are typically used in immunocompromised patients and P. otitidis is often resistant to it. However, its biochemical properties are similar to those of Pseudomonas aeruginosa; therefore, its accurate identification is critical. In the present study, we rapidly identified P. otitidis using MALDI-TOF MS and switched from carbapenems to an appropriate antimicrobial therapy, resulting in a successful outcome.

show abstract

Complete Genome Sequence of Pseudomonas otitidis Strain MrB4, Isolated from Lake Biwa in Japan

Cited by 4 publications

References 16 publications

Pseudomonas otitidis bacteremia in an immunocompromised patient with cellulitis: case report and literature review

Pseudomonas otitidis bacteremia in an immunocompromised patient with cellulitis: case report and literature review

The Rhizobacterium Pseudomonas alcaligenes AVO110 Induces the Expression of Biofilm-Related Genes in Response to Rosellinia necatrix Exudates

Pseudomonas otitidis bacteremia in an immunocompromised patient with cellulitis: case report and literature review

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