Complete Genome Sequence of Pseudomonas putida BS3701, a Promising Polycyclic Aromatic Hydrocarbon-Degrading Strain for Bioremediation Technologies

Филонов, А. Е.; Delegan, Yanina; Пунтус, И. Ф.; Валентович, Л. Н.; Akhremchuk, A. E.; Evdokimova, O. V.; Funtikova, T. V.; Zakharova, Marina; Ахметов, Л. И.; Vetrova, Anna; Титок, М. А.

doi:10.1128/mra.00892-20

Cited by 8 publications

(3 citation statements)

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“…Plasmid DNA isolation has revealed that seven of the effective Rhodococcus strains studied carry plasmids ranging in size from 20 to 1000 bp, and two strains carry two plasmids of 150 and 300 bp ( Figure 1 ). BS3701 has previously been shown to contain two plasmids of 56 and 100 bp [ 9 ]. A. baumannii 7 cells carry small plasmids (6, 12, 20, 42, and 50 kb) [ 8 ].…”

Section: Resultsmentioning

confidence: 99%

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Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation

et al. 2023

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A new biopreparation is developed to clean soils from oil pollution in the arid climate of the Republic of Kazakhstan. The biopreparation includes bacterial strains R. qingshengii F2-1, R. qingshengii F2-2, and P. alloputida BS3701. When using the biopreparation in a liquid mineral medium with 15% crude oil, laboratory studies have revealed degradation of 48% n-alkanes and 39% of PAHs after 50 days. The effectiveness of the biopreparation has been demonstrated in field experiments in the soil contaminated with 10% crude oil at the K-Kurylys landfill, Republic of Kazakhstan. During the six-month field experiment, the number of oil degraders reached 107 CFU/g soil, which degraded 70% of crude oil by the end of the experiment.

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Section: Resultsmentioning

confidence: 99%

“…Pseudomonas alloputida strain BS3701 contains two plasmids (pBS1141 and pBS 1142). Plasmid pBS1141 carries naphthalene biodegradation genes [ 9 ]. R. qingshengii F2-2 contains plasmids harboring genes of n -alkane biodegradation [ 10 , 11 , 12 , 13 ].…”

Section: Figurementioning

confidence: 99%

Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation

et al. 2023

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“…Однако до настоящего времени гены МБЛ P. putida искали только среди внутрибольничных штаммов [7][8][9][10]. В геноме штаммадеструктора компонентов нефти P. putida BS3701 [11] аннотировано 7 генов, ко дирующих белки семейства МБЛподобных металло гидролаз: 6 из них имеют хромосомную локализацию, 1 имеет плазмидную локализацию [12].…”

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Analysis of the genes encoding the MBL-fold metallohydrolase superfamily proteins of the Pseudomonas putida BS3701 petroleum component-degrading strain

Pozdnyakova-Filatova

Zagoskin

Zakharova

et al. 2022

CMAC

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Objective. To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» are related to the proteins of the metallo-β-lactamase group. Materials and Methods. Sequences of the 7 genes annotated as «MBL-fold metallohydrolase superfamily» were analyzed using the following resources: ClustalW, IQ-TREE, iTOL. Selection of the oligonucleotides for real-time PCR was performed using the Primer-BLAST resource. The level of gene expression was assessed using real-time PCR. MIC and MBC measuring was performed using cefepime and meropenem. The double-disc method with EDTA was used to determine the presence of MBL in the strain. Results. Analysis of the nucleotide sequences of the studied genes revealed that all of them were not included in the clade containing sequences of metallo-β-lactamase. In the exponential growth phase, mRNAs corresponding to the studied proteins were found. Determination of MIC and MBC revealed a low level of resistance to antibiotics of the β-lactamase group. The phenotypic test was negative for MBL in P. putida strain BS3701. Conclusions. The investigated genes and corresponding proteins are not related to metallo-β-lactamases. They are not involved in the resistance of P. putida BS3701 to antibiotics of the metallo-β-lactamase group.

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Recent Advances in the Biodegradation of Petroleum Hydrocarbons

Riko,

Darma

2023

Aquatic Contamination

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Complete Genome Sequence of Pseudomonas putida BS3701, a Promising Polycyclic Aromatic Hydrocarbon-Degrading Strain for Bioremediation Technologies

Cited by 8 publications

References 12 publications

Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation

Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation

Analysis of the genes encoding the MBL-fold metallohydrolase superfamily proteins of the Pseudomonas putida BS3701 petroleum component-degrading strain

Recent Advances in the Biodegradation of Petroleum Hydrocarbons

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