Complete Genome Sequence of Ralstonia Phage Remenis, a Member of Putative New Genus within the Siphoviridae

Amarillas, Luis; Estrada-Acosta, Mitzi; León-Chan, Rubén G.; Orona, Carlos Alfonso López; León‐Félix, Josefina; Lightbourn, Luis

doi:10.1007/s12230-020-09793-1

Cited by 1 publication

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“…Of note, during the preparation of the genome architecture comparison figure between the phages related to Nufs112, and other analyses performed within this study, several discrepancies between the results presented in the article describing the phage Reminis [49] and referring to its genome (MN478376) were documented (e.g., no Ralstonia solanacearum was indicated as the host in the metadata of MN478376, the genome of Reminis (MN478376) was not organized taking the SDTRs of 377 bp mentioned by the authors into account, and no SDTRs were present and/or annotated in MN478376, proteome contents and evolutionary analyses using MN478376 indicated that it is a podophage, and not a siphophage, among others).…”

Section: Pantoea Phage Nufs112mentioning

confidence: 86%

Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen

Zrelovs

Jansons

Kazaka

et al. 2023

IJMS

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The bacterial genus Pantoea comprises species found in a variety of different environmental sources. Pantoea spp. are often recovered from plant material and are capable of both benefitting the plants and acting like phytopathogens. Some species of Pantoea (including P. agglomerans) are considered opportunistic human pathogens capable of causing various infections in immunocompromised subjects. In this study, a strain of P. agglomerans (identified by 16S rRNA gene sequencing) was isolated from a dead specimen of an unidentified Latvian grasshopper species. The retrieved strain of P. agglomerans was then used as a host for the potential retrieval of phages from the same source material. After rounds of plaque purification and propagation, three high-titer lysates corresponding to putatively distinct phages were acquired. Transmission electron microscopy revealed that one of the phages was a myophage with an unusual morphology, while the two others were typical podophages. Whole-genome sequencing (WGS) was performed for each of these isolated phages. Genome de novo assembly and subsequent functional annotation confirmed that three different strictly lytic phages were isolated. Elaborate genomic characterization of the acquired phages was performed to elucidate their place within the so-far-uncovered phage diversity.

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Section: Pantoea Phage Nufs112mentioning

confidence: 86%