Complete genome sequence of sixteen plant growth promoting Streptomyces strains

Abstract

The genome sequences of 16 Streptomyces strains, showing potential for plant growth-promotion (PGP) activities in rice, sorghum, chickpea and pigeonpea, isolated from herbal vermicompost, have been decoded. The genome assemblies of the 16 Streptomyces strains ranged from 6.8 Mb to 8.31 Mb, with a GC content of 72 to 73%. The extent of sequence similarity (in terms of shared ortholog) in 16 Streptomyces strains showed 70 to 85% common genes to the closest publicly available Streptomyces genomes. It was possible to identify ~1,850 molecular functions across these 16 strains, of which close to 50% were conserved across the genomes of Streptomyces strains, whereas, ~10% were strain specific and the rest were present in various combinations. Genome assemblies of the 16 Streptomyces strains have also provided genes involved in key pathways related to PGP and biocontrol traits such as siderophores, auxin, hydrocyanic acid, chitinase and cellulase. Further, the genome assemblies provided better understanding of genetic similarity among target strains and with the publically available Streptomyces strains.

Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens

“…can also be observed through their large genome size (more than 8 Mbp with a high G + C content), which is often associated with their ability to survive in various environments [ 159 , 160 ]. Interestingly, genome analysis via genome-mining approach has allowed the prediction of biosynthetic gene clusters related to the plant growth-promoting activities of Streptomyces strains as a biocontrol agent [ 161 ].…”

“…Genomically, the key pathways relating to plant growth-promoting activity, such as siderophores, IAA, HCN, chitinase, and cellulase, have also been decoded in the genome of Streptomyces strains [ 161 ]. It is noteworthy that Streptomyces spp.…”

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

“…Streptomyces sp. is the most important producer of secondary metabolites (39% of the total metabolites produced by microorganisms) (Subramaniam et al 2020). Moreover, 60% of antibiotics, insecticides, and herbicides developed for agriculture use are isolated from Streptomyces (Suwan et al 2012;Vurukonda et al 2018).…”

Potential Role and Utilization of Plant Growth Promoting Microbes in Plant Tissue Culture

“…Some of PGPMs can display two to three plant growth promoting mechanisms. Through their multi-functional roles, PGPMs influence all aspects of plant life including seed germination, nutrition, growth, and response to biotic and/or abiotic stresses ( Weyens et al, 2009 ; Subramaniam et al, 2020a , b ; Sunita et al, 2020 ). PGPMs may enhance plant growth and protection by direct and/or indirect modes of action.…”

“…Like PGPF, they can act as biofertilizers, biostimulants, and/or biocontrol agents. The most widely exploited groups of PGPB belongs to genera of Pseudomonas , Bacillus , Azospirillum , Azotobacter , Rhizobium , Bradyrhizobium , Frankia , Burkholderia , Thiobacillus , Serratia , and Streptomyces ( Adesemoye et al, 2008 ; Sivasakthivelan and Saranraj, 2013 ; Verma et al, 2019 ; Subramaniam et al, 2020a , b ). The different interactions between the roots and surrounding soil PGPMs are summarized in Figure 3 .…”