Complete Genome Sequence of the Corallopyronin A-Producing Myxobacterium Corallococcus coralloides B035

Bouhired, Sarah; Rupp, Oliver; Blom, Jochen; Schäberle, Till F.; Schiefer, Andrea; Kehraus, Stefan; Pfarr, Kenneth; Goesmann, Alexander; Hoerauf, Achim; König, Gabriele M.

doi:10.1128/mra.00050-19

Cited by 4 publications

(2 citation statements)

References 15 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, of the 11 currently sequenced myxobacteria from the genus Corallococcus , 68% of the 621 total putative biosynthetic gene clusters (BGCs) predicted by the analysis platform AntiSMASH are positioned on a contig edge and are potentially incomplete ( Table S2 ). In fact, the only two Corallococcus genomes sequenced with long-read techniques ( Corallococcus coralloides DSM 2259 T and C. coralloides strain B035) each included 34 predicted BGCs with none located on a contig edge [ 41 , 42 ]. Ideally, larger contigs generated from long-read sequencing might benefit the comparative genomic analyses and provide a more complete assessment of biosynthetic potential.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Evolutionary Relationships for Prioritization of Myxobacteria for Natural Product Discovery

et al. 2021

View full text Add to dashboard Cite

Discoveries of novel myxobacteria have started to unveil the potentially vast phylogenetic diversity within the family Myxococcaceae and have brought about an updated approach to myxobacterial classification. While traditional approaches focused on morphology, 16S gene sequences, and biochemistry, modern methods including comparative genomics have provided a more thorough assessment of myxobacterial taxonomy. Herein, we utilize long-read genome sequencing for two myxobacteria previously classified as Archangium primigenium and Chondrococcus macrosporus, as well as four environmental myxobacteria newly isolated for this study. Average nucleotide identity and digital DNA–DNA hybridization scores from comparative genomics suggest previously classified as A. primigenium to instead be a novel member of the genus Melittangium, C. macrosporus to be a potentially novel member of the genus Corallococcus with high similarity to Corallococcus exercitus, and the four isolated myxobacteria to include another novel Corallococcus species, a novel Pyxidicoccus species, a strain of Corallococcus exiguus, and a potentially novel Myxococcus species with high similarity to Myxococcus stipitatus. We assess the biosynthetic potential of each sequenced myxobacterium and suggest that genus-level conservation of biosynthetic pathways support our preliminary taxonomic assignment. Altogether, we suggest that long-read genome sequencing benefits the classification of myxobacteria and improves determination of biosynthetic potential for prioritization of natural product discovery.

show abstract

Section: Introductionmentioning

confidence: 99%

Assessment of Evolutionary Relationships for Prioritization of Myxobacteria for Natural Product Discovery

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The continued discovery of novel, biologically active bacterial metabolites is required to address the need for antimicrobials and anticancer therapeutics. Assessment of biosynthetic space within the growing amount of genome data from myxobacteria can provide insight to direct responsible discovery efforts [72,73,74,75]. This survey likely underestimates the unexplored biosynthetic space from myxobacteria.…”

Section: Discussionmentioning

confidence: 99%

Survey of Biosynthetic Gene Clusters from Sequenced Myxobacteria Reveals Unexplored Biosynthetic Potential

et al. 2019

View full text Add to dashboard Cite

Coinciding with the increase in sequenced bacteria, mining of bacterial genomes for biosynthetic gene clusters (BGCs) has become a critical component of natural product discovery. The order Myxococcales, a reputable source of biologically active secondary metabolites, spans three suborders which all include natural product producing representatives. Utilizing the BiG-SCAPE-CORASON platform to generate a sequence similarity network that contains 994 BGCs from 36 sequenced myxobacteria deposited in the antiSMASH database, a total of 843 BGCs with lower than 75% similarity scores to characterized clusters within the MIBiG database are presented. This survey provides the biosynthetic diversity of these BGCs and an assessment of the predicted chemical space yet to be discovered. Considering the mere snapshot of myxobacteria included in this analysis, these untapped BGCs exemplify the potential for natural product discovery from myxobacteria.

show abstract

Myxobacteria: biology and bioactive secondary metabolites

Saggu

Nath

Kumar

2023

Research in Microbiology

View full text Add to dashboard Cite

Complete Genome Sequence of the Corallopyronin A-Producing Myxobacterium Corallococcus coralloides B035

Cited by 4 publications

References 15 publications

Assessment of Evolutionary Relationships for Prioritization of Myxobacteria for Natural Product Discovery

Assessment of Evolutionary Relationships for Prioritization of Myxobacteria for Natural Product Discovery

Survey of Biosynthetic Gene Clusters from Sequenced Myxobacteria Reveals Unexplored Biosynthetic Potential

Myxobacteria: biology and bioactive secondary metabolites

Contact Info

Product

Resources

About