Complete Genome Sequence of the Oral Pathogenic Bacterium
            <i>Porphyromonas gingivalis</i>
            StrainW83

Nelson, Karen E.; Fleischmann, Robert; DeBoy, Robert T.; Paulsen, Ian T.; Fouts, Derrick E.; Eisen, Jonathan A.; Daugherty, Sean C.; Dodson, Robert J.; Durkin, A. Scott; Gwinn, Michelle L.; Haft, Daniel H.; Kolonay, James F.; Nelson, William; Mason, Tanya; Tallon, Luke J.; Gray, Jessica; Granger, David; Tettelin, Hervé; Hong, Daojun; Galvin, Jamie L.; Duncan, Marilyn J.; Dewhirst, Floyd E.; Fraser, Claire M.

doi:10.1128/jb.186.2.593.2004

Cited by 55 publications

(87 citation statements)

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“…The total database size was 119 Mbytes, the total number of entries was 38,695 proteins. The P. gingivalis ORF sequences consisted of Version 3.1 of the genome annotation by TIGR described by Nelson et al [10] and dated June 8, 2001. See also http://cmr.tigr.org/tigr-scripts/CMR/GenomePage.cgi?org=gpg and http://www.oralgen.lanl.gov/.…”

Section: Sequest and Dtaselectmentioning

confidence: 99%

“…If we take into consideration the genome sequence differences between strains ATCC 33277 and W83 noted in the literature [40] and also the existence of several hundred annotated interposons and other mobile elements [10] that are typically not observed experimentally as proteins, our actual proteome qualitative coverage was on the order of 60% of the protein expressing ORFs. This was significantly higher coverage relative to other literature reports for intracellular pathogens.…”

Section: Qualitative Proteome Coveragementioning

confidence: 99%

“…The completion of the P. gingivalis strain W83 genome sequence in 2001 [10] allowed the application of global expression measurements to the study of P. gingivalis invasion, including proteomic studies [11]. Here we report the observed proteome of P. gingivalis under two biological conditions: intracellular, consisting of organisms recovered from within gingival epithelial cells; and an extracellular reference state consisting of organisms in epithelial cell culture medium that approximates an extracellular milieu in which epithelial cells are physiologically active.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative proteomics of intracellular Porphyromonas gingivalis

Xia¹,

Wang²,

Taub³

et al. 2007

Proteomics

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Whole-cell quantitative proteomic analyses were conducted to investigate the change from an extracellular to intracellular lifestyle for Porphyromonas gingivalis, a Gram-negative intracellular pathogen associated with periodontal disease. Global protein abundance data for P. gingivalis strain ATCC 33277 internalized for 18 hours within human gingival epithelial cells and controls exposed to gingival cell culture medium were obtained at sufficient coverage to provide strong evidence that these changes are profound. A total of 385 proteins were over-expressed in internalized P. gingivalis relative to controls; 240 proteins were shown to be under-expressed. This represented in total about 28% of the protein encoding ORFs annotated for this organism, and slightly less than half of the proteins that were observed experimentally. Production of several proteases, including the classical virulence factors RgpA, RgpB, and Kgp, was decreased. A separate validation study was carried out in which a 16-fold dilution of the P. gingivalis proteome was compared to the undiluted sample in order to assess the quantitative false negative rate (all ratios truly alternative). Truly null (no change) abundance ratios from technical replicates were used to assess the rate of quantitative false positives over the entire proteome. A global comparison between the direction of abundance change observed and previously published bioinformatic gene pair predictions for P. gingivalis will assist with future studies of P. gingivalis gene regulation and operon prediction.

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Section: Sequest and Dtaselectmentioning

confidence: 99%

Section: Qualitative Proteome Coveragementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative proteomics of intracellular Porphyromonas gingivalis

Xia¹,

Wang²,

Taub³

et al. 2007

Proteomics

View full text Add to dashboard Cite

show abstract

“…[42,45,46] PG is an anaerob obligat, [47] non-motile, [48] pleiomorphic bacteria, and posses a capsul. [49] PG shows a strong proteolitic activity, grows in anaerobic environment, and shows dark pigmentation (brown, dark green, or black) on blood agar. [50] PG has fimbriae [51] which mediates adhesion.…”

Section: Literature Reviewmentioning

confidence: 99%

Mechanisms of Periodontitis - Induced Atherosclerosis

Hudyono

Sunariani

2010

IJTID

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Nowadays CVD become the most common cause of death in US and worldwide. Atherosclerosis plays an important role in CVDs pathogenesis. Atherosclerosis decreases the elasticity of the vascular. Atherosclerosis shares the same risk factor as CVD, in which obesity, hyperlipidemia, hypertension and lack of physical activity may initiate it. However, 50% of all CVD patients are lack of the usual causes of CVD. The purpose of this review is to reveal the mechanism of periodontitis-induced atherosclerosis. Inflammation and autoimmune disease might play an important role in initiate the CVD. Periodontitis is one of the oral diseases which can cause systemic inflammation and may induce the atherosclerosis. Porphyromonas gingivalis (Pg) which is the major cause of periodontitis can induce it by expressing protein gp130 in its fimbriae

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“…[8][9][10][11] However, no Dsb family homologs have been found in P. gingivalis W83, whose genes were listed in the TIGR data base. 12) To identify further the potential virulent factors associated with the HBP35, we performed functional analysis of this protein by amino acid substitution. To introduce site-directed mutagenesis, we exploited a simple, rapid, and inexpensive approach based on the utilization of only the T4 DNA polymerase, and all four codons coding for Cys residues present in the hbp35 were successfully altered to ones coding for Ser residues.…”

mentioning

confidence: 99%