Complete Genome Sequence of the Methicillin-Resistant Staphylococcus aureus Colonizing Strain M92

McClure, Jo-Ann; Zhang, Kunyan

doi:10.1128/genomea.00478-17

Cited by 5 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There have been no studies to our knowledge that have characterized the genetic determinants of transmission among MRSA isolates. Our average predicted genome size was 2,857,897 with a range of 2,682,854 bp to 3,009,992 bp among 388 isolates, which is similar to other studies ( 44 ). Large scale BLAST score ratio (LS-BSR) analysis predicted 8,768 genomic coding regions across the 388 MRSA isolates, which differs from previous studies of smaller isolate samples and predictions of pan-genome size ( 44 , 45 ).…”

Section: Discussionsupporting

confidence: 90%

“…Our average predicted genome size was 2,857,897 with a range of 2,682,854 bp to 3,009,992 bp among 388 isolates, which is similar to other studies ( 44 ). Large scale BLAST score ratio (LS-BSR) analysis predicted 8,768 genomic coding regions across the 388 MRSA isolates, which differs from previous studies of smaller isolate samples and predictions of pan-genome size ( 44 , 45 ). Our study allowed the comparison of the genomic content between high and low transmission MRSA isolates, location, and clinical and surveillance isolates.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Comparative Genomics Identifies Features Associated with Methicillin-Resistant Staphylococcus aureus (MRSA) Transmission in Hospital Settings

Adedrian

Hitchcock

O’Hara

et al. 2022

mSphere

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Comparative Genomics Identifies Features Associated with Methicillin-Resistant Staphylococcus aureus (MRSA) Transmission in Hospital Settings

Adedrian

Hitchcock

O’Hara

et al. 2022

mSphere

View full text Add to dashboard Cite

show abstract

“…In the late 1980s, M92, a colonizing MRSA strain and close relative of CMRSA6 and CMRSA3, was isolated from a hospital site in Calgary, AB, Canada. Over the course of many years, M92 was frequently found to be associated with nasal colonization in hospital staff and patients but was never found associated with infections ( Wu et al, 2010 ; McClure and Zhang, 2017 ). In the C. elegans model, this benign strain did not show any nematocidal activity and was, therefore, classed as an avirulent strain and used as a control in many infection models ( Wu et al, 2010 , 2012a , b ).…”

Section: Introductionmentioning

confidence: 99%

Genomic Comparison of Highly Virulent, Moderately Virulent, and Avirulent Strains From a Genetically Closely-Related MRSA ST239 Sub-lineage Provides Insights Into Pathogenesis

et al. 2018

Self Cite

View full text Add to dashboard Cite

The genomic comparison of virulent (TW20), moderately virulent (CMRSA6/CMRSA3), and avirulent (M92) strains from a genetically closely-related MRSA ST239 sub-lineage revealed striking similarities in their genomes and antibiotic resistance profiles, despite differences in virulence and pathogenicity. The main differences were in the spa gene (coding for staphylococcal protein A), lpl genes (coding for lipoprotein-like membrane proteins), cta genes (genes involved in heme synthesis), and the dfrG gene (coding for a trimethoprim-resistant dihydrofolate reductase), as well as variations in the presence or content of some prophages and plasmids, which could explain the virulence differences of these strains. TW20 was positive for all genetic traits tested, compared to CMRSA6, CMRSA3, and M92. The major components differing among these strains included spa and lpl with TW20 carrying both whereas CMRSA6/CMRSA3 carry spa identical to TW20 but have a disrupted lpl. M92 is devoid of both these traits. Considering the role played by these components in innate immunity and virulence, it is predicted that since TW20 has both the components intact and functional, these traits contribute to its pathogenesis. However, CMRSA6/CMRSA3 are missing one of these components, hence their intermediately virulent nature. On the contrary, M92 is completely devoid of both the spa and lpl genes and is avirulent. Mobile genetic elements play a potential role in virulence. TW20 carries three prophages (ϕSa6, ϕSa3, and ϕSPβ-like), a pathogenicity island and two plasmids. CMRSA6, CMRSA3, and M92 contain variations in one or more of these components. The virulence associated genes in these components include staphylokinase, entertoxins, antibiotic/antiseptic/heavy metal resistance and bacterial persistence. Additionally, there are many hypothetical proteins (present with variations among strains) with unknown function in these mobile elements which could be making an important contribution in the virulence of these strains. The above mentioned repertoire of virulence components in TW20 likely contributes to its increased virulence, while the absence and/or modification of one or more of these components in CMRSA6/CMRSA3 and M92 likely affects the virulence of the strains.

show abstract

“…: PRJNA362898), and M92 (GenBank No. : PRJNA319679; CP015447.1) submitted to GenBank and annotated using the prokaryotic genome annotation pipeline [ 24 , 25 ]. Virulence gene profiles for the isolates, including 17 toxin genes, 12 adhesin genes, and 4 exoenzyme genes, were identified and analyzed through the strain whole genome sequences.…”

Section: Methodsmentioning

confidence: 99%

A Murine Skin Infection Model Capable of Differentiating the Dermatopathology of Community-Associated MRSA Strain USA300 from Other MRSA Strains

et al. 2021

Self Cite

View full text Add to dashboard Cite

USA300 is a predominant and highly virulent community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strain that is a leading cause of skin and soft tissue infections. We established a murine intradermal infection model capable of demonstrating dermatopathological differences between USA300 and other MRSA strains. In this model, USA300 induced dermonecrosis, uniformly presenting as extensive open lesions with a histologically documented profound inflammatory cell infiltrate extending below the subcutis. In contrast, USA400 and a colonizing control strain M92 caused only localized non-ulcerated skin infections associated with a mild focal inflammatory infiltrate. It was also determined that the dermonecrosis induced by USA300 was associated with significantly increased neutrophil recruitment, inhibition of an antibacterial response, and increased production of cytokines/chemokines associated with disease severity. These results suggest that induction of severe skin lesions by USA300 is related to over-activation of neutrophils, inhibition of host antibacterial responses, and selective alteration of host cytokine/chemokine profiles.

show abstract

Complete Genome Sequence of the Methicillin-Resistant Staphylococcus aureus Colonizing Strain M92

Cited by 5 publications

References 9 publications

Comparative Genomics Identifies Features Associated with Methicillin-Resistant Staphylococcus aureus (MRSA) Transmission in Hospital Settings

Comparative Genomics Identifies Features Associated with Methicillin-Resistant Staphylococcus aureus (MRSA) Transmission in Hospital Settings

Genomic Comparison of Highly Virulent, Moderately Virulent, and Avirulent Strains From a Genetically Closely-Related MRSA ST239 Sub-lineage Provides Insights Into Pathogenesis

A Murine Skin Infection Model Capable of Differentiating the Dermatopathology of Community-Associated MRSA Strain USA300 from Other MRSA Strains

Contact Info

Product

Resources

About