Complete Genome Sequence of the Urethral Catheter Isolate Myroides sp. A21

Burghartz, Melanie; Bunk, Boyke; Spröer, Cathrin; Voget, Sonja; Daniel, Rolf; Overmann, Jörg; Jahn, Martina

doi:10.1128/genomea.00068-15

Cited by 10 publications

(6 citation statements)

References 10 publications

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“…For the phylogenetic tree analysis of Myroides genomes, another human‐associated strain Myroides sp. A21 (CP010327) (Burghartz et al., ) with highly homologous 16S rRNA gene sequence to strain PR63039 (coverage 100%, identity 100%, 1,388 bp) (GenBank No. KR349266) was also included.…”

Section: Methodsmentioning

confidence: 99%

Comparative genomic analysis of Myroides odoratimimus isolates

Chen

Yi-yin

et al. 2018

MicrobiologyOpen

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Myroides odoratimimus is an important nosocomial pathogen. Management of M. odoratimimus infection is difficult owing to the multidrug resistance and the unknown pathogenesis mechanisms. Based on our previous genomic sequencing data of M. odoratimimus PR63039 (isolated from a patient with the urinary tract infection), in this study, we further performed comparative genomic analysis for 10 selected Myroides strains. Our results showed that these Myroides genome contexts were very similar and phylogenetically related. Various prophages were identified in the four clinical isolate genomes, which possibly contributed to the genome evolution among the Myroides strains. CRISPR elements were only detected in the two clinical (PR63039 and CCUG10230) isolates and two environmental (CCUG12700 and H1bi) strains. With more stringent cutoff parameters in CARD analysis, the four clinical M. odoratimimus contained roughly equal antibiotic resistance genes, indicating their similar antibiotic resistance profiles. The three clinical (CCUG10230, CCUG12901, CIP101113) and three environmental (CCUG12700, L41, H1bi) M. odoratimimus strains were speculated to carry the indistinguishable virulent factors (VFs), which may involve in the similar pathogenesis mechanism. Moreover, some VFs might confer to the high capacity of dissemination, attacking tissue cells and induction of autoimmune complications. Our results facilitate the research of antibiotic resistance and the development of therapeutic regimens for the M. odoratimimus infections.

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Section: Methodsmentioning

confidence: 99%

Comparative genomic analysis of Myroides odoratimimus isolates

Chen

Yi-yin

et al. 2018

MicrobiologyOpen

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“…By comparing the genome sequence of Myroides sp. A21 with those of M. odoratimimus CCUG 10230 and M. odoratus DSM 2801, 293 unique CDSs were found in the A21 genome (Burghartz et al, 2015). In addition, five genomic islands were predicted by Island Viewer analysis (Burghartz et al, 2015).…”

Section: Whole Genome Sequencing Is a Feasible Way To Investigate Thementioning

confidence: 99%

“…A21 with those of M. odoratimimus CCUG 10230 and M. odoratus DSM 2801, 293 unique CDSs were found in the A21 genome (Burghartz et al, 2015). In addition, five genomic islands were predicted by Island Viewer analysis (Burghartz et al, 2015). However, as the antibiotic treatment history was not described and the antibiotic resistance status of this strain was not given, the antibiotic resistance mechanisms could not be analyzed from the data.…”

Section: Whole Genome Sequencing Is a Feasible Way To Investigate Thementioning

confidence: 99%

“…Recently, the genome sequencing of the urethral catheter isolate Myroides sp. A21 was completed (Burghartz et al, 2015). The sequence contained 3650 protein-coding sequences (CDSs), 136 RNAcoding genes, and eight copies of the rRNA gene cluster, of which three were resolved as a direct repeat of two rRNA gene clusters.…”

Section: Whole Genome Sequencing Is a Feasible Way To Investigate Thementioning

confidence: 99%

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Antibiotic resistance mechanisms of Myroides sp.

Yuan

et al. 2016

J. Zhejiang Univ. Sci. B

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Bacteria of the genus Myroides (Myroides spp.) are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA (rRNA) gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that obtaining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infections. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.

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“…The genus Myroides , proposed as belonging to the family Flavobacteriaceae , was established with the reclassification of the species Flavobacterium odoratum ( 1 ). Members of this genus are nonfermenting, Gram-negative, aerobic, and nonmotile bacteria ( 2 ) that previously have been discovered only from clinical sources, aquatic environment, grey mullet’s gut, and flesh flies ( 3 – 5 ). A new Myroides strain, N17-2, was first isolated from radiation-polluted soils in Xinjiang, Uyghur Autonomous Region, China.…”

Section: Genome Announcementmentioning

confidence: 99%