Complete Genome Sequence of the Polysaccharide-Degrading Rumen Bacterium<i>Pseudobutyrivibrio xylanivorans</i>MA3014

Palevich, Nikola; Maclean, Paul; Kelly, William J.; Leahy, Sinead C.; Rakonjac, Jasna; Attwood, Graeme T.

doi:10.1101/2020.07.08.194233

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…In our study, Prevotella which was the dominant bacteria being responsible for starch fermentation to produce propionate in the rumen (Strobel, 1992), was positively correlated with propionic acid content. Pseudobutyrivibrio can digest and degrade some complex plant polysaccharides and then produce VFA for use by ruminants (Palevich et al, 2020). It is easy to understand that the relative abundance of Pseudobutyrivibrio was negatively correlated with the pH value observed in our study.…”

Section: Correlations Of Rumen Bacterial Abundances With Fermentation...supporting

confidence: 47%

Effects of waste milk feeding on rumen fermentation and bacterial community of pre-weaned and post-weaned dairy calves

Zhang

Cheng

et al. 2023

Front. Microbiol.

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The objective of this study was to investigate the effect of waste milk with antibiotic residue on rumen fermentation and rumen bacterial composition of dairy calves during pre-weaned and post-weaned periods. A total of 24 Holstein male calves (43.4 ± 0.93 kg body weight, mean ± standard error) were allocated into four blocks based on birth date. Dairy calves were supplied 100% milk replacer (MR, n = 8), 50% milk replacer mixed with 50% waste milk (MM, n = 8), or 100% waste milk (WM, n = 8). Ruminal samples were collected at 49 and 63 days of age and then subjected to determinations of pH value, volatile fatty acids (VFA), ammonia nitrogen (NH3–N) and 16S rRNA gene amplicon sequencing. The results showed that feeding WM had no effect on the pH value, the concentrations of VFA (acetic acid, propionic acid, butyric acid, isovaleric acid, valeric acid), and NH3–N in dairy calves compared to feeding MR. However, from 49 to 63 days of age, the pH value (p < 0.001) was significantly increased, while the levels of total VFA (p = 0.004), acetic acid (p = 0.01), propionic acid (p = 0.003) and valeric acid (p < 0.001) were significantly decreased. For rumen microorganisms, there was no differences in bacterial diversity among the treatments. But the relative abundance of Veillonellaceae was significantly lower (p = 0.05) in the calves fed WM than that from MR group at 49 days of age; however, no difference was detected at 63 days of age. Feeding WM to calves tended to reduce family Veillonellaceae and genus Olsenella in the rumen at 49 days of age (p = 0.049). Analysis of temporal changes in rumen bacteria based on alpha-diversity and beta-diversity as well as the microbial relative abundances did not exhibit any difference. In addition, relative abundances of Clostridia_UCG-014, Prevotella, Syntrophococcus, Eubacterium_nodatum_group, Pseudoramibacter and Solobacterium were correlated with rumen pH value and the concentrations of TVFA, propionic acid, isovaleric acid, valeric acid and NH3–N. In conclusion, compare to MR, calves supplied with WM had little changes on the rumen pH value, NH3–N or VFAs contents. Additionally, limited effects could be found on rumen microbiota in the calves fed WM. However, further studies needed to explore if there exist any long-term effects of early-life rumen microbiota modulation on dairy cows.

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Section: Correlations Of Rumen Bacterial Abundances With Fermentation...supporting

confidence: 47%

Effects of waste milk feeding on rumen fermentation and bacterial community of pre-weaned and post-weaned dairy calves

Zhang

Cheng

et al. 2023

Front. Microbiol.

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Comparative genomics ofClostridiumspecies associated with vacuum-packed meat spoilage

Palevich

Maclean

et al. 2020

Preprint

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Bacterial species belonging to the Clostridium genera have been recognized as causative agents of blown pack spoilage (BPS) in vacuum packed meat products. Whole-genome sequencing of six New Zealand psychrotolerant Clostridium isolates derived from three meat production animal types and their environments was performed to examine their roles in BPS. Comparative genome analyses have provided insight into the genomic diversity and physiology of these bacteria and divides Clostridia into two separate species clusters. BPS-associated Clostridia encode a large and diverse spectrum of degradative carbohydrate-active enzymes (CAZymes). In total, 516 glycoside hydrolases (GHs), 93 carbohydrate esterases (CEs), 21 polysaccharide lyases (PLs), 434 glycosyl transferases (GTs) and 211 carbohydrate-binding protein modules (CBM) with predicted activities involved in the breakdown and transport of carbohydrates were identified. Clostridia genomes have different patterns of CAZyme families and vary greatly in the number of genes within each CAZy category, suggesting some level of functional redundancy. These results suggest that BPS-associated Clostridia occupy similar environmental niches but apply different carbohydrate metabolism strategies to be able to co-exist and cause meat spoilage.

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Complete Genome Sequence of the Polysaccharide-Degrading Rumen BacteriumPseudobutyrivibrio xylanivoransMA3014

Cited by 2 publications

References 37 publications

Effects of waste milk feeding on rumen fermentation and bacterial community of pre-weaned and post-weaned dairy calves

Effects of waste milk feeding on rumen fermentation and bacterial community of pre-weaned and post-weaned dairy calves

Comparative genomics ofClostridiumspecies associated with vacuum-packed meat spoilage

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