Complete Genome Sequence of the Persistent Listeria monocytogenes Strain R479a

Schmitz‐Esser, Stephan; Gram, Lone; Wagner, Martin

doi:10.1128/genomea.00150-15

Cited by 14 publications

(16 citation statements)

References 7 publications

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“…For comparative genomic analysis of the sequenced strains, L . monocytogenes R479a [ 16 ] was selected as the reference strain. This strain was the only ST8 strain for which a complete genome sequence was available.…”

Section: Resultsmentioning

confidence: 99%

“…These were the complete sequences of the L . monocytogenes R479a chromosome and pLMR479a plasmid, which were used as reference sequences [ 16 ], and draft genomes of L . monocytogenes strains SHL004 [ 25 ], Lm_1823, Lm_1889 [ 20 ], and Lm21045 ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…L . monocytogenes of MLST sequence type 8 (ST8) was identified as persisting for over three years in a raw salmon processing plant in Denmark [ 15 , 16 ], and is commonly found in food products and food processing environments [ 17 – 21 ]. L .…”

Section: Introductionmentioning

confidence: 99%

“…The genomes of the five currently sequenced strains were furthermore compared with five additional available ST8 genomes, of which one was a persistent clone isolated from a Danish salmon processing facility [ 15 , 16 ], two were isolated from ricotta cheese in Italy [ 20 ], and one was a clinical strain isolated from a hospitalized patient in Shanghai, China [ 25 ]. Inclusion of these strains in the study enabled examination of ST8 strains isolated from a range of food and food processing environments (salmon, poultry, and cheese) in addition to a human clinical strain, and allowed the reconstruction of the evolutionary history of the ST8 strains using SNP data.…”

Section: Introductionmentioning

confidence: 99%

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Genome Analysis of Listeria monocytogenes Sequence Type 8 Strains Persisting in Salmon and Poultry Processing Environments and Comparison with Related Strains

et al. 2016

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Listeria monocytogenes is an important foodborne pathogen responsible for the disease listeriosis, and can be found throughout the environment, in many foods and in food processing facilities. The main cause of listeriosis is consumption of food contaminated from sources in food processing environments. Persistence in food processing facilities has previously been shown for the L. monocytogenes sequence type (ST) 8 subtype. In the current study, five ST8 strains were subjected to whole-genome sequencing and compared with five additionally available ST8 genomes, allowing comparison of strains from salmon, poultry and cheese industry, in addition to a human clinical isolate. Genome-wide analysis of single-nucleotide polymorphisms (SNPs) confirmed that almost identical strains were detected in a Danish salmon processing plant in 1996 and in a Norwegian salmon processing plant in 2001 and 2011. Furthermore, we show that L. monocytogenes ST8 was likely to have been transferred between two poultry processing plants as a result of relocation of processing equipment. The SNP data were used to infer the phylogeny of the ST8 strains, separating them into two main genetic groups. Within each group, the plasmid and prophage content was almost entirely conserved, but between groups, these sequences showed strong divergence. The accessory genome of the ST8 strains harbored genetic elements which could be involved in rendering the ST8 strains resilient to incoming mobile genetic elements. These included two restriction-modification loci, one of which was predicted to show phase variable recognition sequence specificity through site-specific domain shuffling. Analysis indicated that the ST8 strains harbor all important known L. monocytogenes virulence factors, and ST8 strains are commonly identified as the causative agents of invasive listeriosis. Therefore, the persistence of this L. monocytogenes subtype in food processing facilities poses a significant concern for food safety.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome Analysis of Listeria monocytogenes Sequence Type 8 Strains Persisting in Salmon and Poultry Processing Environments and Comparison with Related Strains

et al. 2016

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“…Notably, the stress survival characteristics of L. monocytogenes 6179 have been extensively characterized (Müller et al, 2013(Müller et al, , 2014Casey et al, 2014;Makariti et al, 2015;Rychli et al, 2016;Harter et al, 2017;Naditz et al, 2019). L. monocytogenes strain R479a is an ST8 strain isolated from smoked salmon in Denmark in 1996 where it persisted for more than 2 years in the same FPE (Fonnesbech Vogel et al, 2001;Schmitz-Esser et al, 2015a). L. monocytogenes R479a possesses a 2.94 Mbp genome and a 86.6 kbp plasmid, pLMR479a.…”

Section: Bacterial Strains Used In This Studymentioning

confidence: 99%

Transcriptome Sequencing of Listeria monocytogenes Reveals Major Gene Expression Changes in Response to Lactic Acid Stress Exposure but a Less Pronounced Response to Oxidative Stress

et al. 2020

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Cortes et al. Listeria Stress Transcriptomics σ B regulon, particularly lmo2230 and the non-coding RNA Rli47, play an integral role in the response of L. monocytogenes to acid stress. Furthermore, we report the first global transcriptome sequencing analysis of L. monocytogenes plasmid gene expression and identify a putative, plasmid-encoded riboswitch with potential involvement in response to acid exposure.

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ON‐rep‐seq as a rapid and cost‐effective alternative to whole‐genome sequencing for species‐level identification and strain‐level discrimination of Listeria monocytogenes contamination in a salmon processing plant

et al. 2021

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Complete Genome Sequence of the Persistent Listeria monocytogenes Strain R479a

Abstract: The complete genome sequence of the persistent Listeria monocytogenes strain R479a isolated from smoked salmon in Denmark and belonging to lineage II, serovar 1/2a, and multilocus sequence type 8 (ST8) is presented here.

Cited by 14 publications

References 7 publications

Genome Analysis of Listeria monocytogenes Sequence Type 8 Strains Persisting in Salmon and Poultry Processing Environments and Comparison with Related Strains

Genome Analysis of Listeria monocytogenes Sequence Type 8 Strains Persisting in Salmon and Poultry Processing Environments and Comparison with Related Strains

Transcriptome Sequencing of Listeria monocytogenes Reveals Major Gene Expression Changes in Response to Lactic Acid Stress Exposure but a Less Pronounced Response to Oxidative Stress

ON‐rep‐seq as a rapid and cost‐effective alternative to whole‐genome sequencing for species‐level identification and strain‐level discrimination of Listeria monocytogenes contamination in a salmon processing plant

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