Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer

Abstract

Tepidimonas taiwanensis is a moderately thermophilic, Gram-negative, rod-shaped, chemoorganoheterotrophic, motile bacterium. The alkaline protease producing type strain Tepidimonas taiwanensis LMG 22826T was recently reported to also be a promising producer of polyhydroxyalkanoates (PHAs) – renewable and biodegradable polymers representing an alternative to conventional plastics. Here, we present its first complete genome sequence which is also the first complete genome sequence of the whole species. The genome consists of a single 2,915,587 bp long circular chromosome with GC content of 68.75%. Genome annotation identified 2,764 genes in total while 2,634 open reading frames belonged to protein-coding genes. Although functional annotation of the genome and division of genes into Clusters of Orthologous Groups (COGs) revealed a relatively high number of 694 genes with unknown function or unknown COG, the majority of genes were assigned a function. Most of the genes, 406 in total, were involved in energy production and conversion, and amino acid transport and metabolism. Moreover, particular key genes involved in the metabolism of PHA were identified. Knowledge of the genome in connection with the recently reported ability to produce bioplastics from the waste stream of wine production makes T. taiwanensis LMG 22826T an ideal candidate for further genome engineering as a bacterium with high biotechnological potential.

The Pathway Analysis of Tepidimonas taiwanensis LMG 22826T, Polyhydroxyalkanoate and Alkaline Protease Producer

“…Another industrially utilizable property of this bacterium is alkaline protease production. The ability of alkaline proteases production by T. tawanensis LMG 22826 T was first found experimentally [7], and later was confirmed based on a prediction of molecular weights from protein sequences derived from genome assembly [8]. The genome sequence revealed the presence of genes coding many alkaline proteases but only three of them encoded for extracellular proteases that are used in many industries, such as detergent or medical industry [9].…”

“…Apart from serine protease, the genome of T. taiwanensis also has the gene encoding lysyl endopeptidase (LCC91_10460, EC:3.4.21.50) and protease IV (LCC91_01535, EC:3.4.21.-). The first mentioned is produced by many bacteria and has been already selling as a commercial product and protease IV has suitable properties for usage in the detergent industry [8].…”